KRTAP1-1 siRNA – Keratin-Associated Protein 1-1 (Human)

Name: KRTAP1-1 siRNA; Keratin-Associated Protein 1-1; Human; MyBioSource
Brand: MyBioSource
SKU: MBS8230627
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8230627

MBS8230627 is an siRNA targeting KRTAP1-1. Also known as KRTAP1-1 siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit KRTAP1-1 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

KRTAP1-1, siRNA

Full Product Name

KRTAP1-1 siRNA (Human)

Product Gene Name

KRTAP1-1 sirna

Product Synonym Names

B2A; KAP1.1; KAP1.6; KAP1.7; KRTAP1.1; Keratin-associated protein 1-1; High sulfur keratin-associated protein 1.1; Keratin-associated protein 1.1; Keratin-associated protein 1.6; Keratin-associated protein 1.7

Host

Synthetic

Species Reactivity

Human

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

KRTAP1-1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

18,235 Da

OMIM

608819

3D Structure

ModBase 3D Structure for Q07627

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human KRTAP1-1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for KRTAP1-1 sirna

RNA Interference (RNAi)

Other Notes

Small volumes of KRTAP1-1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for KRTAP1-1. It may not necessarily be applicable to this product.

NCBI GI #

13569942

NCBI GeneID

81851

NCBI Accession #

NP_112229.1

UniProt Primary Accession #

Q07627

UniProt Secondary Accession #

Q96S60; Q96S67; A6NC32

UniProt Related Accession #

Q07627

GenBank Nucleotide #

NM_030967.2

NCBI Official Full Name

keratin-associated protein 1-1

NCBI Official Synonym Full Names

keratin associated protein 1-1

NCBI Official Symbol

KRTAP1-1

NCBI Official Synonym Symbols

HB2A; KAP1.1; KAP1.6; KAP1.7; KAP1.1A; KAP1.1B; KRTAP1A; hKAP1.7; KRTAP1.1

UniProt Protein Name

Keratin-associated protein 1-1

UniProt Synonym Protein Names

High sulfur keratin-associated protein 1.1; Keratin-associated protein 1.1; Keratin-associated protein 1.6; Keratin-associated protein 1.7

Protein Family

Keratin-associated protein

UniProt Gene Name

KRTAP1-1

UniProt Synonym Gene Names

B2A; KAP1.1; KAP1.6; KAP1.7; KRTAP1.1

UniProt Entry Name

KRA11_HUMAN

NCBI Protein Information

keratin-associated protein 1-1

NCBI Summary for KRTAP1-1

This protein is a member of the keratin-associated protein (KAP) family. The KAP proteins form a matrix of keratin intermediate filaments which contribute to the structure of hair fibers. KAP family members appear to have unique, family-specific amino- and carboxyl-terminal regions and are subdivided into three multi-gene families according to amino acid composition: the high sulfur, the ultrahigh sulfur, and the high tyrosine/glycine KAPs. This protein is a member of the high sulfur KAP family and the gene is localized to a cluster of KAPs at 17q12-q21. [provided by RefSeq, Jul 2008]

UniProt Comments for KRTAP1-1

KRTAP1-1: In the hair cortex, hair keratin intermediate filaments are embedded in an interfilamentous matrix, consisting of hair keratin-associated proteins (KRTAP), which are essential for the formation of a rigid and resistant hair shaft through their extensive disulfide bond cross-linking with abundant cysteine residues of hair keratins. The matrix proteins include the high- sulfur and high-glycine-tyrosine keratins. Belongs to the KRTAP type 1 family. Interacts with hair keratins

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: keratin filament

Research Articles on KRTAP1-1

1. Krtap11-1 may play an important role on keratin-bundle assembly in the hair cortex.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.