LRWD1 siRNA – Leucine-Rich Repeat and Wd Repeat-Containing Protein 1 (Human)

Name: LRWD1 siRNA; Human; MyBioSource
Brand: MyBioSource
SKU: MBS8201543
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8201543

MBS8201543 is an siRNA targeting LRWD1. Also known as LRWD1 siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit LRWD1 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

LRWD1, siRNA

Full Product Name

LRWD1 siRNA (Human)

Product Gene Name

LRWD1 sirna

Product Synonym Names

CENP33; ORCA; Leucine-rich repeat and WD repeat-containing protein 1; Centromere protein 33; CENP-33; Origin recognition complex-associated protein; ORC-associated protein; ORCA

Host

Synthetic

Species Reactivity

Human

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

LRWD1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

70,861 Da

OMIM

615167

3D Structure

ModBase 3D Structure for Q9UFC0

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human LRWD1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for LRWD1 sirna

RNA Interference (RNAi)

Other Notes

Small volumes of LRWD1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for LRWD1. It may not necessarily be applicable to this product.

NCBI GI #

23097240

NCBI GeneID

222229

NCBI Accession #

NP_690852.1

UniProt Primary Accession #

Q9UFC0

UniProt Secondary Accession #

Q8N0T9; Q8WV43; Q96GJ2; A8K4K2; B2R9G2

UniProt Related Accession #

Q9UFC0

GenBank Nucleotide #

NM_152892.1

NCBI Official Full Name

leucine-rich repeat and WD repeat-containing protein 1

NCBI Official Synonym Full Names

leucine-rich repeats and WD repeat domain containing 1

NCBI Official Symbol

LRWD1

NCBI Official Synonym Symbols

ORCA; CENP-33

UniProt Protein Name

Leucine-rich repeat and WD repeat-containing protein 1

UniProt Synonym Protein Names

Centromere protein 33; CENP-33; Origin recognition complex-associated protein; ORC-associated protein; ORCA

Protein Family

Leucine-rich repeat and WD repeat-containing protein

UniProt Gene Name

LRWD1

UniProt Synonym Gene Names

CENP33; ORCA; CENP-33; ORC-associated protein; ORCA

UniProt Entry Name

LRWD1_HUMAN

NCBI Protein Information

leucine-rich repeat and WD repeat-containing protein 1

UniProt Comments for LRWD1

LRWD1: Associated component of the origin recognition complex (ORC) required to recruit and stabilize the ORC complex to chromatin. Probably required for the association of ORC on chromatin during G1 to establish pre-replication complex (preRC) and to heterochromatic sites in post-replicated cells. Binds a combination of DNA and histone methylation repressive marks on heterochromatin: binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3 in a cooperative manner with DNA methylation. It is unclear whether it recongnizes and binds these repressive marks by itself or needs additional factors. Belongs to the LRWD1 family.

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: centric heterochromatin; cytoplasm; microtubule organizing center; nuclear origin of replication recognition complex; nucleus

Molecular Function: protein binding; methyl-CpG binding; chromatin binding; methylated histone residue binding

Biological Process: establishment and/or maintenance of chromatin architecture; DNA replication initiation; chromatin modification

Research Articles on LRWD1

1. Data (from studies using recombinant proteins) suggest that deletion of leucine rich (LRR) domain significantly down-regulates expression of LRWD1 protein; LRR domain is significantly correlated with stability of LRWD1 protein.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with LRWD1 sirna

Reference Product	PubMed Publications
ORC2 sirna	>3 publications with LRWD1 and ORC2
ORC1 sirna	>1 publications with LRWD1 and ORC1

Diseases associated with LRWD1 sirna

Disease Name	Pubmed Publications
Necrosis Antibodies	>1 publications with LRWD1 and Necrosis

Organs/Tissues associated with LRWD1 sirna

Organ/Tissue Name	Pubmed Publications
Testis Antibodies	>4 publications with LRWD1 and Testis