LSM14A siRNA – Protein LSM14 Homolog A Isoform a (Human)

Name: LSM14A siRNA; Protein LSM14 Homolog A Isoform a; Human; MyBioSource
Brand: MyBioSource
SKU: MBS8206085
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8206085

MBS8206085 is an siRNA targeting LSM14A. Also known as LSM14A siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit LSM14A expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

LSM14A, siRNA

Full Product Name

LSM14A siRNA (Human)

Product Gene Name

LSM14A sirna

Product Synonym Names

C19orf13; FAM61A; RAP55; RAP55A; Protein LSM14 homolog A; Protein FAM61A; Protein SCD6 homolog; Putative alpha-synuclein-binding protein; AlphaSNBP; RNA-associated protein 55A; hRAP55; hRAP55A

Host

Synthetic

Species Reactivity

Human

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

LSM14A siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

46,411 Da

OMIM

610677

3D Structure

ModBase 3D Structure for Q8ND56

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human LSM14A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for LSM14A sirna

RNA Interference (RNAi)

Other Notes

Small volumes of LSM14A sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for LSM14A. It may not necessarily be applicable to this product.

NCBI GI #

166197712

NCBI GeneID

26065

NCBI Accession #

NP_001107565.1

UniProt Primary Accession #

Q8ND56

UniProt Secondary Accession #

Q76LX7; Q96AR3; Q96K73; Q96SN5; Q9UFR3; B4DTG6

UniProt Related Accession #

Q8ND56

GenBank Nucleotide #

NM_001114093.1

NCBI Official Full Name

protein LSM14 homolog A isoform a

NCBI Official Synonym Full Names

LSM14A mRNA processing body assembly factor

NCBI Official Symbol

LSM14A

NCBI Official Synonym Symbols

RAP55; FAM61A; RAP55A; C19orf13

UniProt Protein Name

Protein LSM14 homolog A

UniProt Synonym Protein Names

Protein FAM61A; Protein SCD6 homolog; Putative alpha-synuclein-binding protein; AlphaSNBP; RNA-associated protein 55A; hRAP55; hRAP55A

Protein Family

Protein

UniProt Gene Name

LSM14A

UniProt Synonym Gene Names

C19orf13; FAM61A; RAP55; RAP55A; AlphaSNBP; hRAP55; hRAP55A

UniProt Entry Name

LS14A_HUMAN

NCBI Protein Information

protein LSM14 homolog A

NCBI Summary for LSM14A

Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family (see SNRPD2; 601061). Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing.[supplied by OMIM, Mar 2008]

UniProt Comments for LSM14A

alphaSNBP(A): Essential for formation of P-bodies, cytoplasmic structures that provide storage sites for non-translating mRNAs. Belongs to the LSM14 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Translation; RNA-binding

Chromosomal Location of Human Ortholog: 19q13.11

Cellular Component: intracellular membrane-bound organelle; stress granule; cytoplasm

Molecular Function: single-stranded RNA binding; double-stranded DNA binding; double-stranded RNA binding

Biological Process: regulation of translation; multicellular organismal development; cytoplasmic mRNA processing body assembly

Research Articles on LSM14A

1. RAP55 is important for the assembly of cytoplasmic mRNP granules and that PRMT1 is required for RAP55A to localize to P-bodies.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with LSM14A sirna

Reference Product	PubMed Publications
DDX6 sirna	>3 publications with LSM14A and DDX6
EDC4 sirna	>2 publications with LSM14A and EDC4
EDC3 sirna	>1 publications with LSM14A and EDC3
DCP2 sirna	>1 publications with LSM14A and DCP2

Diseases associated with LSM14A sirna

Disease Name	Pubmed Publications
Necrosis Antibodies	>1 publications with LSM14A and Necrosis

Organs/Tissues associated with LSM14A sirna

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>1 publications with LSM14A and Blood
Brain Antibodies	>1 publications with LSM14A and Brain