MAP6 siRNA – Microtubule-Associated Protein 6 Isoform 1 (Human)

Name: MAP6 siRNA; Human; MyBioSource
Brand: MyBioSource
SKU: MBS8201168
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8201168

MBS8201168 is an siRNA targeting MAP6. Also known as MAP6 siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit MAP6 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

MAP6, siRNA

Full Product Name

MAP6 siRNA (Human)

Product Gene Name

MAP6 sirna

Product Synonym Names

KIAA1878; Microtubule-associated protein 6; MAP-6; Stable tubule-only polypeptide; STOP

Host

Synthetic

Species Reactivity

Human

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

MAP6 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

51,329 Da

OMIM

601783

3D Structure

ModBase 3D Structure for Q96JE9

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MAP6 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for MAP6 sirna

RNA Interference (RNAi)

Other Notes

Small volumes of MAP6 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MAP6. It may not necessarily be applicable to this product.

NCBI GI #

48375173

NCBI GeneID

4135

NCBI Accession #

NP_149052.1

UniProt Primary Accession #

Q96JE9

UniProt Secondary Accession #

Q6P3T0; Q6ZWB8; A7E2A1

UniProt Related Accession #

Q96JE9

GenBank Nucleotide #

NM_033063.1

NCBI Official Full Name

microtubule-associated protein 6 isoform 1

NCBI Official Synonym Full Names

microtubule-associated protein 6

NCBI Official Symbol

MAP6

NCBI Official Synonym Symbols

STOP; MTAP6; MAP6-N; N-STOP

UniProt Protein Name

Microtubule-associated protein 6

UniProt Synonym Protein Names

Stable tubule-only polypeptide; STOP

Protein Family

Microtubule-associated protein

UniProt Gene Name

MAP6

UniProt Synonym Gene Names

KIAA1878; MAP-6; STOP

UniProt Entry Name

MAP6_HUMAN

NCBI Protein Information

microtubule-associated protein 6

NCBI Summary for MAP6

This gene encodes a microtubule-associated protein. The encoded protein is a calmodulin-binding and calmodulin-regulated protein that is involved in microtubule stabilization. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

UniProt Comments for MAP6

MAP6: Involved in microtubule stabilization in many cell types, including neuronal cells. Specifically has microtubule cold stabilizing activity. Interacts with calmodulin (via C-terminus); the interaction is dependent on Ca(2+). Expressed in brain. Expressed in spinal chord. Isoform 2 expression is up-regulated in the prefrontal cortex (Brodmann's area 46) of patients with schizophrenia (postmortem brain study). Belongs to the STOP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 11q13.5

Cellular Component: Golgi apparatus; microtubule; perinuclear region of cytoplasm

Molecular Function: calmodulin binding; protein binding; microtubule binding

Biological Process: lysosome localization; transport; dendrite morphogenesis; microtubule cytoskeleton organization and biogenesis

Research Articles on MAP6

1. These data show that TMEM106B/MAP6 interaction is crucial for controlling dendritic trafficking of lysosomes, presumably by acting as a molecular brake for retrograde transport.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with MAP6 sirna

Reference Product	PubMed Publications
MAP4 sirna	>2 publications with MAP6 and MAP4
TMEM106B sirna	>2 publications with MAP6 and TMEM106B

Diseases associated with MAP6 sirna

Disease Name	Pubmed Publications
Disease Models, Animal Antibodies	>5 publications with MAP6 and Disease Models, Animal
Male Urogenital Diseases Antibodies	>2 publications with MAP6 and Male Urogenital Diseases
Nerve Degeneration Antibodies	>2 publications with MAP6 and Nerve Degeneration
Prostatic Diseases Antibodies	>2 publications with MAP6 and Prostatic Diseases
Cognition Disorders Antibodies	>2 publications with MAP6 and Cognition Disorders
Anxiety Disorders Antibodies	>2 publications with MAP6 and Anxiety Disorders
Learning Disorders Antibodies	>2 publications with MAP6 and Learning Disorders
Memory Disorders Antibodies	>1 publications with MAP6 and Memory Disorders
Atrophy Antibodies	>1 publications with MAP6 and Atrophy

Organs/Tissues associated with MAP6 sirna

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>19 publications with MAP6 and Brain
Nerve Antibodies	>12 publications with MAP6 and Nerve
Prostate Antibodies	>2 publications with MAP6 and Prostate
Kidney Antibodies	>1 publications with MAP6 and Kidney