MMP15 siRNA – Matrix Metalloproteinase-15 Preproprotein (Human)

Name: MMP15 siRNA; Human; MyBioSource
Brand: MyBioSource
SKU: MBS8207693
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8207693

MBS8207693 is an siRNA targeting MMP15. Also known as MMP15 siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit MMP15 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

MMP15, siRNA

Full Product Name

MMP15 siRNA (Human)

Product Gene Name

MMP15 sirna

Product Synonym Names

Matrix metalloproteinase-15; MMP-15; Membrane-type matrix metalloproteinase 2; MT-MMP 2; MTMMP2; Membrane-type-2 matrix metalloproteinase; MT2-MMP; MT2MMP; SMCP-2

Host

Synthetic

Species Reactivity

Human

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

MMP15 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

75,807 Da

OMIM

602261

3D Structure

ModBase 3D Structure for P51511

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MMP15 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for MMP15 sirna

RNA Interference (RNAi)

Other Notes

Small volumes of MMP15 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MMP15. It may not necessarily be applicable to this product.

NCBI GI #

4505211

NCBI GeneID

4324

NCBI Accession #

NP_002419.1

UniProt Primary Accession #

P51511

UniProt Secondary Accession #

Q14111; A0A2U6

UniProt Related Accession #

P51511

GenBank Nucleotide #

NM_002428.2

NCBI Official Full Name

matrix metalloproteinase-15 preproprotein

NCBI Official Synonym Full Names

matrix metallopeptidase 15 (membrane-inserted)

NCBI Official Symbol

MMP15

NCBI Official Synonym Symbols

MTMMP2; SMCP-2; MT2-MMP

UniProt Protein Name

Matrix metalloproteinase-15

UniProt Synonym Protein Names

Membrane-type matrix metalloproteinase 2; MT-MMP 2; MTMMP2; Membrane-type-2 matrix metalloproteinase; MT2-MMP; MT2MMP; SMCP-2

Protein Family

Matrix metalloproteinase

UniProt Gene Name

MMP15

UniProt Synonym Gene Names

MMP-15; MT-MMP 2; MTMMP2; MT2-MMP; MT2MMP

UniProt Entry Name

MMP15_HUMAN

NCBI Protein Information

matrix metalloproteinase-15

NCBI Summary for MMP15

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. [provided by RefSeq, Jul 2008]

UniProt Comments for MMP15

MMP15: Endopeptidase that degrades various components of the extracellular matrix. May activate progelatinase A. Belongs to the peptidase M10A family.

Protein type: EC 3.4.24.-; Protease; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16q13

Cellular Component: extracellular matrix; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; zinc ion binding; metalloendopeptidase activity; enzyme activator activity; calcium ion binding

Biological Process: positive regulation of catalytic activity; extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; protein modification process; proteolysis; response to estradiol stimulus

Research Articles on MMP15

1. our data suggest that MT2-MMP expression positively involves in non-small cell lung cancer and might play an important role in promoting the tumor progression and intra-tumoral angiogenesis

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with MMP15 sirna

Reference Product	PubMed Publications
MMP9 sirna	>10 publications with MMP15 and MMP9
MMP2 sirna	>9 publications with MMP15 and MMP2
MMP8 sirna	>3 publications with MMP15 and MMP8
TIMP2 sirna	>2 publications with MMP15 and TIMP2
TIMP1 sirna	>2 publications with MMP15 and TIMP1
MMP7 sirna	>1 publications with MMP15 and MMP7
MMP19 sirna	>1 publications with MMP15 and MMP19

Pathways associated with MMP15 sirna

Products by Pathway	Pathway Diagram
Activation Of Matrix Metalloproteinases Pathway antibodies	Activation Of Matrix Metalloproteinases Pathway Diagram
Collagen Degradation Pathway antibodies	Collagen Degradation Pathway Diagram
Degradation Of The Extracellular Matrix Pathway antibodies	Degradation Of The Extracellular Matrix Pathway Diagram
Extracellular Matrix Organization Pathway antibodies	Extracellular Matrix Organization Pathway Diagram
Matrix Metalloproteinases Pathway antibodies	Matrix Metalloproteinases Pathway Diagram

Diseases associated with MMP15 sirna

Disease Name	Pubmed Publications
Adenocarcinoma Antibodies	>8 publications with MMP15 and Adenocarcinoma
Breast Neoplasms Antibodies	>7 publications with MMP15 and Breast Neoplasms
Cell Transformation, Neoplastic Antibodies	>5 publications with MMP15 and Cell Transformation, Neoplastic
Lung Diseases Antibodies	>5 publications with MMP15 and Lung Diseases
Necrosis Antibodies	>4 publications with MMP15 and Necrosis
Vascular Diseases Antibodies	>3 publications with MMP15 and Vascular Diseases
Liver Diseases Antibodies	>2 publications with MMP15 and Liver Diseases
Disease Models, Animal Antibodies	>2 publications with MMP15 and Disease Models, Animal
Fibrosis Antibodies	>2 publications with MMP15 and Fibrosis
Neoplasms, Experimental Antibodies	>2 publications with MMP15 and Neoplasms, Experimental

Organs/Tissues associated with MMP15 sirna

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>10 publications with MMP15 and Lung
Embryonic Tissue Antibodies	>8 publications with MMP15 and Embryonic Tissue
Vascular Antibodies	>8 publications with MMP15 and Vascular
Blood Antibodies	>8 publications with MMP15 and Blood
Skin Antibodies	>7 publications with MMP15 and Skin
Placenta Antibodies	>6 publications with MMP15 and Placenta
Brain Antibodies	>6 publications with MMP15 and Brain
Bone Antibodies	>5 publications with MMP15 and Bone
Liver Antibodies	>4 publications with MMP15 and Liver
Muscle Antibodies	>3 publications with MMP15 and Muscle