MND1 siRNA – Meiotic Nuclear Division Protein 1 Homolog Isoform 1 (Human)

Name: MND1 siRNA; Human; MyBioSource
Brand: MyBioSource
SKU: MBS8203628
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8203628

MBS8203628 is an siRNA targeting MND1. Also known as MND1 siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit MND1 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

MND1, siRNA

Full Product Name

MND1 siRNA (Human)

Product Gene Name

MND1 sirna

Product Synonym Names

GAJ; Meiotic nuclear division protein 1 homolog

Host

Synthetic

Species Reactivity

Human

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

MND1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

23,753 Da

OMIM

611422

3D Structure

ModBase 3D Structure for Q9BWT6

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MND1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for MND1 sirna

RNA Interference (RNAi)

Other Notes

Small volumes of MND1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MND1. It may not necessarily be applicable to this product.

NCBI GI #

14149769

NCBI GeneID

84057

NCBI Accession #

NP_115493.1

UniProt Primary Accession #

Q9BWT6

UniProt Secondary Accession #

B2R5F9

UniProt Related Accession #

Q9BWT6

GenBank Nucleotide #

NM_032117.3

NCBI Official Full Name

meiotic nuclear division protein 1 homolog isoform 1

NCBI Official Synonym Full Names

meiotic nuclear divisions 1

NCBI Official Symbol

MND1

NCBI Official Synonym Symbols

GAJ

UniProt Protein Name

Meiotic nuclear division protein 1 homolog

Protein Family

Meiotic nuclear division protein

UniProt Gene Name

MND1

UniProt Entry Name

MND1_HUMAN

NCBI Protein Information

meiotic nuclear division protein 1 homolog

NCBI Summary for MND1

The product of the MND1 gene associates with HOP2 (MIM 608665) to form a stable heterodimeric complex that binds DNA and stimulates the recombinase activity of RAD51 (MIM 179617) and DMC1 (MIM 602721) (Chi et al., 2007 [PubMed 17639080]). Both the MND1 and HOP2 genes are indispensable for meiotic recombination.[supplied by OMIM, Mar 2008]

UniProt Comments for MND1

MND1: Required for proper homologous chromosome pairing and efficient cross-over and intragenic recombination during meiosis. Stimulates both DMC1- and RAD51-mediated homologous strand assimilation, which is required for the resolution of meiotic double-strand breaks. Belongs to the MND1 family.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 4q31.3

Cellular Component: nucleus

Molecular Function: DNA binding

Biological Process: meiotic cell cycle; DNA recombination

Research Articles on MND1

1. Data indicate that the positive expression of studied genes fertilization rate for GAPDHS positive subset was 66%, ACR - 71%, SPATA22 - 68%, MND1 - 70%, pregnancy rates were 8%, 6%, 18% and 36% respectively.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with MND1 sirna

Reference Product	PubMed Publications
DMC1 sirna	>28 publications with MND1 and DMC1
RAD51 sirna	>26 publications with MND1 and RAD51
PSMC3IP sirna	>4 publications with MND1 and PSMC3IP
BRCA2 sirna	>3 publications with MND1 and BRCA2
ATM sirna	>1 publications with MND1 and ATM
MSH4 sirna	>1 publications with MND1 and MSH4

Pathways associated with MND1 sirna

Products by Pathway	Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Meiosis Pathway antibodies	Meiosis Pathway Diagram
Meiotic Recombination Pathway antibodies	Meiotic Recombination Pathway Diagram

Diseases associated with MND1 sirna

Disease Name	Pubmed Publications
Kidney Diseases Antibodies	>4 publications with MND1 and Kidney Diseases
Urogenital Abnormalities Antibodies	>1 publications with MND1 and Urogenital Abnormalities
Congenital Abnormalities Antibodies	>1 publications with MND1 and Congenital Abnormalities

Organs/Tissues associated with MND1 sirna

Organ/Tissue Name	Pubmed Publications
Testis Antibodies	>2 publications with MND1 and Testis