NDRG4 siRNA – Protein NDRG4 Isoform 2 (Human)

Name: NDRG4 siRNA; Protein NDRG4 Isoform 2; Human; MyBioSource
Brand: MyBioSource
SKU: MBS8205342
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8205342

MBS8205342 is an siRNA targeting NDRG4. Also known as NDRG4 siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit NDRG4 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

NDRG4, siRNA

Full Product Name

NDRG4 siRNA (Human)

Product Gene Name

NDRG4 sirna

Product Synonym Names

BDM1; KIAA1180; Protein NDRG4; Brain development-related molecule 1; N-myc downstream-regulated gene 4 protein; Vascular smooth muscle cell-associated protein 8; SMAP-8

Host

Synthetic

Species Reactivity

Human

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

NDRG4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

39,168 Da

OMIM

614463

3D Structure

ModBase 3D Structure for Q9ULP0

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human NDRG4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for NDRG4 sirna

RNA Interference (RNAi)

Other Notes

Small volumes of NDRG4 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NDRG4. It may not necessarily be applicable to this product.

NCBI GI #

194440722

NCBI GeneID

65009

NCBI Accession #

NP_001123959.1

UniProt Primary Accession #

Q9ULP0

UniProt Secondary Accession #

Q6ZNE7; Q6ZTI7; Q96PL9; Q9GZM1; Q9GZN3; Q9GZX0; B3KNU2; B4DK66; B4DSW5; H7C600

UniProt Related Accession #

Q9ULP0

GenBank Nucleotide #

NM_001130487.1

NCBI Official Full Name

protein NDRG4 isoform 2

NCBI Official Synonym Full Names

NDRG family member 4

NCBI Official Symbol

NDRG4

NCBI Official Synonym Symbols

BDM1; SMAP8; SMAP-8

UniProt Protein Name

Protein NDRG4

UniProt Synonym Protein Names

Brain development-related molecule 1; N-myc downstream-regulated gene 4 protein; Vascular smooth muscle cell-associated protein 8; SMAP-8

Protein Family

Protein

UniProt Gene Name

NDRG4

UniProt Synonym Gene Names

BDM1; KIAA1180; SMAP-8

UniProt Entry Name

NDRG4_HUMAN

NCBI Protein Information

protein NDRG4

NCBI Summary for NDRG4

This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein that is required for cell cycle progression and survival in primary astrocytes and may be involved in the regulation of mitogenic signalling in vascular smooth muscles cells. Alternative splicing results in multiple transcripts encoding different isoforms.[provided by RefSeq, Jun 2011]

UniProt Comments for NDRG4

NDRG4: Contributes to the maintenance of intracerebral BDNF levels within the normal range, which is necessary for the preservation of spatial learning and the resistance to neuronal cell death caused by ischemic stress. May enhance growth factor-induced ERK1 and ERK2 phosphorylation, including that induced by PDGF and FGF. May attenuate NGF-promoted ELK1 phosphorylation in a microtubule-dependent manner. Expressed predominantly in brain and heart. In the brain, detected in astrocytes. Isoform 1 and isoform 2 are only expressed in brain. Isoform 3 is expressed in both heart and brain. Up-regulated in glioblastoma multiforme cells. Belongs to the NDRG family. 6 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 16q21

Cellular Component: endoplasmic reticulum membrane; mitochondrion; basolateral plasma membrane; cytoplasm; cytosol

Biological Process: negative regulation of smooth muscle cell proliferation; embryonic heart tube development; response to stress; cardiac muscle cell proliferation; negative regulation of smooth muscle cell migration; heart looping; cell growth; cell differentiation

Research Articles on NDRG4

1. A homozygous variant in NDRG4 may be the causative variant of the autosomal recessive form of infantile myofibromatosis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with NDRG4 sirna

Reference Product	PubMed Publications
MYCN sirna	>1 publications with NDRG4 and MYCN
ALCAM sirna	>1 publications with NDRG4 and ALCAM

Diseases associated with NDRG4 sirna

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>7 publications with NDRG4 and Nervous System Diseases
Carcinoma Antibodies	>5 publications with NDRG4 and Carcinoma
Glioma Antibodies	>3 publications with NDRG4 and Glioma
Breast Neoplasms Antibodies	>2 publications with NDRG4 and Breast Neoplasms
Nerve Degeneration Antibodies	>1 publications with NDRG4 and Nerve Degeneration
Liver Diseases Antibodies	>1 publications with NDRG4 and Liver Diseases
Learning Disorders Antibodies	>1 publications with NDRG4 and Learning Disorders
Memory Disorders Antibodies	>1 publications with NDRG4 and Memory Disorders
Hyperplasia Antibodies	>1 publications with NDRG4 and Hyperplasia
Neoplasms, Experimental Antibodies	>1 publications with NDRG4 and Neoplasms, Experimental

Organs/Tissues associated with NDRG4 sirna

Organ/Tissue Name	Pubmed Publications
Nerve Antibodies	>30 publications with NDRG4 and Nerve
Muscle Antibodies	>30 publications with NDRG4 and Muscle
Brain Antibodies	>17 publications with NDRG4 and Brain
Heart Antibodies	>10 publications with NDRG4 and Heart
Embryonic Tissue Antibodies	>6 publications with NDRG4 and Embryonic Tissue
Pancreas Antibodies	>3 publications with NDRG4 and Pancreas
Prostate Antibodies	>3 publications with NDRG4 and Prostate
Testis Antibodies	>2 publications with NDRG4 and Testis
Intestine Antibodies	>1 publications with NDRG4 and Intestine
Lymph Node Antibodies	>1 publications with NDRG4 and Lymph Node