NDUFA2 siRNA – NADH Dehydrogenase (Human)

Name: NDUFA2 siRNA; NADH Dehydrogenase; Human; MyBioSource
Brand: MyBioSource
SKU: MBS828923
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS828923

MBS828923 is an siRNA targeting NDUFA2. Also known as NDUFA2 siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit NDUFA2 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

NDUFA2, siRNA

Full Product Name

NDUFA2 siRNA (Human)

Product Gene Name

NDUFA2 sirna

Product Synonym Names

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2; Complex I-B8; CI-B8; NADH-ubiquinone oxidoreductase B8 subunit

Host

Synthetic

Species Reactivity

Human

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

NDUFA2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

8,491 Da

OMIM

602137

3D Structure

ModBase 3D Structure for O43678

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human NDUFA2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for NDUFA2 sirna

RNA Interference (RNAi)

Other Notes

Small volumes of NDUFA2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NDUFA2. It may not necessarily be applicable to this product.

NCBI GI #

297206774

NCBI GeneID

4695

NCBI Accession #

NP_001171941.1

UniProt Primary Accession #

O43678

UniProt Secondary Accession #

Q6IAY8; D6RJD6

UniProt Related Accession #

O43678

GenBank Nucleotide #

NM_001185012.1

NCBI Official Full Name

NADH dehydrogenase

NCBI Official Synonym Full Names

NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 2, 8kDa

NCBI Official Symbol

NDUFA2

NCBI Official Synonym Symbols

B8; CD14; CIB8

UniProt Protein Name

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2

UniProt Synonym Protein Names

Complex I-B8; CI-B8; NADH-ubiquinone oxidoreductase B8 subunit

UniProt Gene Name

NDUFA2

UniProt Synonym Gene Names

CI-B8

UniProt Entry Name

NDUA2_HUMAN

NCBI Protein Information

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2

NCBI Summary for NDUFA2

The encoded protein is a subunit of the hydrophobic protein fraction of the NADH:ubiquinone oxidoreductase (complex 1), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane, and may be involved in regulating complex I activity or its assembly via assistance in redox processes. Mutations in this gene are associated with Leigh syndrome, an early-onset progressive neurodegenerative disorder. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

UniProt Comments for NDUFA2

NDUFA2: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Protein type: EC 1.6.99.3; Oxidoreductase; Energy Metabolism - oxidative phosphorylation; Mitochondrial; EC 1.6.5.3

Chromosomal Location of Human Ortholog: 5q31.2

Cellular Component: mitochondrial inner membrane; mitochondrial membrane; mitochondrial respiratory chain complex I

Molecular Function: NADH dehydrogenase (ubiquinone) activity

Biological Process: cellular metabolic process; mitochondrial electron transport, NADH to ubiquinone

Disease: Leigh Syndrome

Research Articles on NDUFA2

1. Observational study of gene-disease association. (HuGE Navigator)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with NDUFA2 sirna

Reference Product	PubMed Publications
NDUFS8 sirna	>2 publications with NDUFA2 and NDUFS8
NDUFV2 sirna	>2 publications with NDUFA2 and NDUFV2
NDUFA7 sirna	>2 publications with NDUFA2 and NDUFA7
NDUFB8 sirna	>2 publications with NDUFA2 and NDUFB8

Pathways associated with NDUFA2 sirna

Products by Pathway	Pathway Diagram
Alzheimer's Disease Pathway antibodies	Alzheimer's Disease Pathway Diagram
Alzheimer's Disease Pathway antibodies	Alzheimer's Disease Pathway Diagram
Electron Transport Chain Pathway antibodies	Electron Transport Chain Pathway Diagram
Huntington's Disease Pathway antibodies	Huntington's Disease Pathway Diagram
Huntington's Disease Pathway antibodies	Huntington's Disease Pathway Diagram
Metabolic Pathways antibodies	Metabolic Pathways Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
NADH Dehydrogenase (ubiquinone) 1 Alpha Subcomplex Pathway antibodies	NADH Dehydrogenase (ubiquinone) 1 Alpha Subcomplex Pathway Diagram
NADH Dehydrogenase (ubiquinone) 1 Alpha Subcomplex Pathway antibodies	NADH Dehydrogenase (ubiquinone) 1 Alpha Subcomplex Pathway Diagram
Non-alcoholic Fatty Liver Disease (NAFLD) Pathway antibodies	Non-alcoholic Fatty Liver Disease (NAFLD) Pathway Diagram

Diseases associated with NDUFA2 sirna

Disease Name	Pubmed Publications
Leigh Disease Antibodies	>1 publications with NDUFA2 and Leigh Disease

Organs/Tissues associated with NDUFA2 sirna

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>2 publications with NDUFA2 and Muscle
Bone Antibodies	>1 publications with NDUFA2 and Bone
Blood Antibodies	>1 publications with NDUFA2 and Blood
Bone Marrow Antibodies	>1 publications with NDUFA2 and Bone Marrow
Skin Antibodies	>1 publications with NDUFA2 and Skin
Heart Antibodies	>1 publications with NDUFA2 and Heart