NSA2 siRNA – Ribosome Biogenesis Protein NSA2 Homolog Isoform 2 (Human)

Name: NSA2 siRNA; Human; MyBioSource
Brand: MyBioSource
SKU: MBS8204979
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8204979

MBS8204979 is an siRNA targeting NSA2. Also known as NSA2 siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit NSA2 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

NSA2, siRNA

Full Product Name

NSA2 siRNA (Human)

Product Gene Name

NSA2 sirna

Product Synonym Names

TINP1; Ribosome biogenesis protein NSA2 homolog; Hairy cell leukemia protein 1; TGF-beta-inducible nuclear protein 1

Host

Synthetic

Species Reactivity

Human

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

NSA2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

30,065 Da

OMIM

612497

3D Structure

ModBase 3D Structure for O95478

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human NSA2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for NSA2 sirna

RNA Interference (RNAi)

Other Notes

Small volumes of NSA2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NSA2. It may not necessarily be applicable to this product.

NCBI GI #

410651502

NCBI GeneID

10412

NCBI Accession #

NP_001258594.1

UniProt Primary Accession #

O95478

UniProt Related Accession #

O95478

GenBank Nucleotide #

NM_001271665.1

NCBI Official Full Name

ribosome biogenesis protein NSA2 homolog isoform 2

NCBI Official Synonym Full Names

NSA2 ribosome biogenesis homolog

NCBI Official Symbol

NSA2

NCBI Official Synonym Symbols

HCLG1; TINP1; CDK105; HCL-G1; HUSSY29; HUSSY-29

UniProt Protein Name

Ribosome biogenesis protein NSA2 homolog

UniProt Synonym Protein Names

Hairy cell leukemia protein 1; TGF-beta-inducible nuclear protein 1

Protein Family

Ribosome biogenesis protein

UniProt Gene Name

NSA2

UniProt Synonym Gene Names

TINP1

UniProt Entry Name

NSA2_HUMAN

NCBI Protein Information

ribosome biogenesis protein NSA2 homolog

NCBI Summary for NSA2

This gene encodes a nucleolar protein involved in cell cycle regulation and proliferation. This gene was identified based on sequence similarity to a highly conserved Saccharomyces cerevisiae gene encoding a pre-ribosomal protein, which is involved in large ribosomal subunit biogenesis. The encoded protein is found at elevated levels in diabetic nephropathy. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified. [provided by RefSeq, Nov 2012]

UniProt Comments for NSA2

TINP1: Involved in the biogenesis of the 60S ribosomal subunit. May play a part in the quality control of pre-60S particles. Belongs to the ribosomal protein S8e family.

Protein type: Nucleolus; Translation

Chromosomal Location of Human Ortholog: 5q13.3

Cellular Component: nucleolus; ribonucleoprotein complex; nucleus

Biological Process: rRNA processing

Research Articles on NSA2

1. TINP1 promotes cell proliferation and significantly reduces p53 and p21 expression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with NSA2 sirna

Reference Product	PubMed Publications
RPF2 sirna	>1 publications with NSA2 and RPF2

Diseases associated with NSA2 sirna

Disease Name	Pubmed Publications
Diabetes Complications Antibodies	>2 publications with NSA2 and Diabetes Complications
Kidney Diseases Antibodies	>2 publications with NSA2 and Kidney Diseases
Immune System Diseases Antibodies	>1 publications with NSA2 and Immune System Diseases

Organs/Tissues associated with NSA2 sirna

Organ/Tissue Name	Pubmed Publications
Kidney Antibodies	>2 publications with NSA2 and Kidney
Blood Antibodies	>1 publications with NSA2 and Blood