NTSR2 siRNA – Neurotensin Receptor Type 2 (Human)

Name: NTSR2 siRNA; Neurotensin Receptor Type 2; Human; MyBioSource
Brand: MyBioSource
SKU: MBS8216947
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8216947

MBS8216947 is an siRNA targeting NTSR2. Also known as NTSR2 siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit NTSR2 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

NTSR2, siRNA

Full Product Name

NTSR2 siRNA (Human)

Product Gene Name

NTSR2 sirna

Product Synonym Names

Neurotensin receptor type 2; NT-R-2; NTR2; Levocabastine-sensitive neurotensin receptor

Host

Synthetic

Species Reactivity

Human

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

NTSR2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

45,385 Da

OMIM

605538

3D Structure

ModBase 3D Structure for O95665

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human NTSR2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for NTSR2 sirna

RNA Interference (RNAi)

Other Notes

Small volumes of NTSR2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NTSR2. It may not necessarily be applicable to this product.

NCBI GI #

6912538

NCBI GeneID

23620

NCBI Accession #

NP_036476.1

UniProt Primary Accession #

O95665

UniProt Secondary Accession #

Q53QQ5; Q57Z87; Q8IY58; Q8TBH6

UniProt Related Accession #

O95665

GenBank Nucleotide #

NM_012344.3

NCBI Official Full Name

neurotensin receptor type 2

NCBI Official Synonym Full Names

neurotensin receptor 2

NCBI Official Symbol

NTSR2

NCBI Official Synonym Symbols

NTR2

UniProt Protein Name

Neurotensin receptor type 2

UniProt Synonym Protein Names

Levocabastine-sensitive neurotensin receptor

Protein Family

Neurotensin receptor

UniProt Gene Name

NTSR2

UniProt Synonym Gene Names

NT-R-2; NTR2

UniProt Entry Name

NTR2_HUMAN

NCBI Protein Information

neurotensin receptor type 2

NCBI Summary for NTSR2

The protein encoded by this gene belongs to the G protein-coupled receptor family that activate a phosphatidylinositol-calcium second messenger system. Binding and pharmacological studies demonstrate that this receptor binds neurotensin as well as several other ligands already described for neurotensin NT1 receptor. However, unlike NT1 receptor, this gene recognizes, with high affinity, levocabastine, a histamine H1 receptor antagonist previously shown to compete with neurotensin for low-affinity binding sites in brain. These activities suggest that this receptor may be of physiological importance and that a natural agonist for the receptor may exist. [provided by RefSeq, Jul 2008]

UniProt Comments for NTSR2

NTSR2: neurotensin receptor 2

Protein type: Receptor, GPCR; Membrane protein, multi-pass; GPCR, family 1; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2p25.1

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; neurotensin receptor activity, G-protein coupled

Biological Process: regulation of membrane potential; cell surface receptor linked signal transduction; neuropeptide signaling pathway; sensory perception; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating)

Research Articles on NTSR2

1. NTSR2 was overexpressed, NTSR1 decreased, and neurotensin was not expressed in B cell leukemia patient's B-cells, as compared with healthy B cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with NTSR2 sirna

Reference Product	PubMed Publications
NTS sirna	>12 publications with NTSR2 and NTS

Pathways associated with NTSR2 sirna

Products by Pathway	Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
G Alpha (q) Signalling Events Pathway antibodies	G Alpha (q) Signalling Events Pathway Diagram
GPCR Downstream Signaling Pathway antibodies	GPCR Downstream Signaling Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram
GPCRs, Class A Rhodopsin-like Pathway antibodies	GPCRs, Class A Rhodopsin-like Pathway Diagram
Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies	Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
Neuroactive Ligand-receptor Interaction Pathway antibodies	Neuroactive Ligand-receptor Interaction Pathway Diagram
Neuroactive Ligand-receptor Interaction Pathway antibodies	Neuroactive Ligand-receptor Interaction Pathway Diagram
Peptide GPCRs Pathway antibodies	Peptide GPCRs Pathway Diagram
Peptide Ligand-binding Receptors Pathway antibodies	Peptide Ligand-binding Receptors Pathway Diagram

Diseases associated with NTSR2 sirna

Disease Name	Pubmed Publications
Hypothermia Antibodies	>4 publications with NTSR2 and Hypothermia
Neurodegenerative Diseases Antibodies	>2 publications with NTSR2 and Neurodegenerative Diseases
Memory Disorders Antibodies	>2 publications with NTSR2 and Memory Disorders
Learning Disorders Antibodies	>2 publications with NTSR2 and Learning Disorders
Disease Models, Animal Antibodies	>1 publications with NTSR2 and Disease Models, Animal
Hyperglycemia Antibodies	>1 publications with NTSR2 and Hyperglycemia
Inflammation Antibodies	>1 publications with NTSR2 and Inflammation

Organs/Tissues associated with NTSR2 sirna

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>41 publications with NTSR2 and Brain
Ganglia Antibodies	>4 publications with NTSR2 and Ganglia