PLXNC1 siRNA – Plexin-C1 (Human)

Name: PLXNC1 siRNA; Plexin-C1; Human; MyBioSource
Brand: MyBioSource
SKU: MBS8228783
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8228783

MBS8228783 is an siRNA targeting PLXNC1. Also known as PLXNC1 siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit PLXNC1 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PLXNC1, siRNA

Full Product Name

PLXNC1 siRNA (Human)

Product Gene Name

PLXNC1 sirna

Product Synonym Names

VESPR; Plexin-C1; Virus-encoded semaphorin protein receptor; CD antigen CD232

Host

Synthetic

Species Reactivity

Human

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

PLXNC1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

175,742 Da

OMIM

604259

3D Structure

ModBase 3D Structure for O60486

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PLXNC1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for PLXNC1 sirna

RNA Interference (RNAi)

Other Notes

Small volumes of PLXNC1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PLXNC1. It may not necessarily be applicable to this product.

NCBI GI #

5032223

NCBI GeneID

10154

NCBI Accession #

NP_005752.1

UniProt Primary Accession #

O60486

UniProt Secondary Accession #

Q59H25

UniProt Related Accession #

O60486

GenBank Nucleotide #

NM_005761.2

NCBI Official Full Name

plexin-C1

NCBI Official Synonym Full Names

plexin C1

NCBI Official Symbol

PLXNC1

NCBI Official Synonym Symbols

CD232; VESPR; PLXN-C1

UniProt Protein Name

Plexin-C1

UniProt Synonym Protein Names

Virus-encoded semaphorin protein receptor; CD_antigen: CD232

Protein Family

Plexin

UniProt Gene Name

PLXNC1

UniProt Synonym Gene Names

VESPR

UniProt Entry Name

PLXC1_HUMAN

NCBI Protein Information

plexin-C1

NCBI Summary for PLXNC1

This gene encodes a member of the plexin family. Plexins are transmembrane receptors for semaphorins, a large family of proteins that regulate axon guidance, cell motility and migration, and the immune response. The encoded protein and its ligand regulate melanocyte adhesion, and viral semaphorins may modulate the immune response by binding to this receptor. The encoded protein may be a tumor suppressor protein for melanoma. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jan 2011]

UniProt Comments for PLXNC1

PLXNC1: Receptor for SEMA7A, for smallpox semaphorin A39R, vaccinia virus semaphorin A39R and for herpesvirus Sema protein. Binding of semaphorins triggers cellular responses leading to the rearrangement of the cytoskeleton and to secretion of IL6 and IL8. Belongs to the plexin family.

Protein type: Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q23.3

Cellular Component: membrane; integral to membrane; plasma membrane; intracellular

Molecular Function: protein binding; receptor activity; receptor binding

Biological Process: axon guidance; cell adhesion; signal transduction

Research Articles on PLXNC1

1. loss of Plexin C1 in melanoma may promote early steps in melanoma progression through suppression of migration and proliferation

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PLXNC1 sirna

Reference Product	PubMed Publications
SEMA7A sirna	>3 publications with PLXNC1 and SEMA7A
SEMA3A sirna	>1 publications with PLXNC1 and SEMA3A

Pathways associated with PLXNC1 sirna

Products by Pathway	Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram
Other Semaphorin Interactions Pathway antibodies	Other Semaphorin Interactions Pathway Diagram
Semaphorin Interactions Pathway antibodies	Semaphorin Interactions Pathway Diagram

Diseases associated with PLXNC1 sirna

Disease Name	Pubmed Publications
Inflammation Antibodies	>2 publications with PLXNC1 and Inflammation
Pancreatic Neoplasms Antibodies	>1 publications with PLXNC1 and Pancreatic Neoplasms
Disease Models, Animal Antibodies	>1 publications with PLXNC1 and Disease Models, Animal

Organs/Tissues associated with PLXNC1 sirna

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>2 publications with PLXNC1 and Lung
Eye Antibodies	>1 publications with PLXNC1 and Eye
Mouth Antibodies	>1 publications with PLXNC1 and Mouth
Testis Antibodies	>1 publications with PLXNC1 and Testis
Embryonic Tissue Antibodies	>1 publications with PLXNC1 and Embryonic Tissue
Blood Antibodies	>1 publications with PLXNC1 and Blood
Kidney Antibodies	>1 publications with PLXNC1 and Kidney
Brain Antibodies	>1 publications with PLXNC1 and Brain