PON2 siRNA – Serum Paraoxonase/Arylesterase 2 Isoform 1 (Human)

Name: PON2 siRNA; Human; MyBioSource
Brand: MyBioSource
SKU: MBS8239955
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8239955

MBS8239955 is an siRNA targeting PON2. Also known as PON2 siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit PON2 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PON2, siRNA

Full Product Name

PON2 siRNA (Human)

Product Gene Name

PON2 sirna

Product Synonym Names

Serum paraoxonase/arylesterase 2; PON 2; Aromatic esterase 2; A-esterase 2; Serum aryldialkylphosphatase 2

Host

Synthetic

Species Reactivity

Human

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

PON2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

37,996 Da

OMIM

602447

3D Structure

ModBase 3D Structure for Q15165

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PON2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for PON2 sirna

RNA Interference (RNAi)

Other Notes

Small volumes of PON2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PON2. It may not necessarily be applicable to this product.

NCBI GI #

66529294

NCBI GeneID

5445

NCBI Accession #

NP_000296.2

UniProt Primary Accession #

Q15165

UniProt Secondary Accession #

O15114; O15115; O75856; Q5FBX7; Q86YL0; A4D1H7; B2RCP9; B4DJD5

UniProt Related Accession #

Q15165

GenBank Nucleotide #

NM_000305.2

NCBI Official Full Name

serum paraoxonase/arylesterase 2 isoform 1

NCBI Official Synonym Full Names

paraoxonase 2

NCBI Official Symbol

PON2

UniProt Protein Name

Serum paraoxonase/arylesterase 2

UniProt Synonym Protein Names

Aromatic esterase 2; A-esterase 2; Serum aryldialkylphosphatase 2

Protein Family

Serum paraoxonase/arylesterase

UniProt Gene Name

PON2

UniProt Synonym Gene Names

PON 2; A-esterase 2

UniProt Entry Name

PON2_HUMAN

NCBI Protein Information

serum paraoxonase/arylesterase 2

NCBI Summary for PON2

This gene encodes a member of the paraoxonase gene family, which includes three known members located adjacent to each other on the long arm of chromosome 7. The encoded protein is ubiquitously expressed in human tissues, membrane-bound, and may act as a cellular antioxidant, protecting cells from oxidative stress. Hydrolytic activity against acylhomoserine lactones, important bacterial quorum-sensing mediators, suggests the encoded protein may also play a role in defense responses to pathogenic bacteria. Mutations in this gene may be associated with vascular disease and a number of quantitative phenotypes related to diabetes. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

UniProt Comments for PON2

PON2: Capable of hydrolyzing lactones and a number of aromatic carboxylic acid esters. Has antioxidant activity. Is not associated with high density lipoprotein. Prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits the ability of MM-LDL to induce monocyte chemotaxis. Belongs to the paraoxonase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; EC 3.1.1.2; EC 3.1.1.81; Hydrolase

Chromosomal Location of Human Ortholog: 7q21.3

Cellular Component: mitochondrion; lysosome; plasma membrane; extracellular region; nucleus

Molecular Function: identical protein binding; arylesterase activity; metal ion binding

Biological Process: response to oxidative stress; aromatic compound catabolic process

Research Articles on PON2

1. A modest dose-dependent allele effect between the PON2 c.311C > G polymorphism, increased triglyceride concentrations and decreased LDL particle size distribution.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PON2 sirna

Reference Product	PubMed Publications
APOE sirna	>16 publications with PON2 and APOE
MTHFR sirna	>6 publications with PON2 and MTHFR

Pathways associated with PON2 sirna

Products by Pathway	Pathway Diagram
Metabolic Pathways antibodies	Metabolic Pathways Diagram
Phase I, Non P450 Pathway antibodies	Phase I, Non P450 Pathway Diagram

Diseases associated with PON2 sirna

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>113 publications with PON2 and Cardiovascular Diseases
Atherosclerosis Antibodies	>80 publications with PON2 and Atherosclerosis
Heart Diseases Antibodies	>35 publications with PON2 and Heart Diseases
Brain Diseases Antibodies	>31 publications with PON2 and Brain Diseases
Inflammation Antibodies	>20 publications with PON2 and Inflammation
Hypertension Antibodies	>13 publications with PON2 and Hypertension
Kidney Diseases Antibodies	>12 publications with PON2 and Kidney Diseases
Myocardial Infarction Antibodies	>7 publications with PON2 and Myocardial Infarction
Liver Diseases Antibodies	>7 publications with PON2 and Liver Diseases
Fatty Liver Antibodies	>5 publications with PON2 and Fatty Liver

Organs/Tissues associated with PON2 sirna

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>88 publications with PON2 and Blood
Liver Antibodies	>36 publications with PON2 and Liver
Heart Antibodies	>29 publications with PON2 and Heart
Vascular Antibodies	>28 publications with PON2 and Vascular
Brain Antibodies	>19 publications with PON2 and Brain
Kidney Antibodies	>15 publications with PON2 and Kidney
Lung Antibodies	>11 publications with PON2 and Lung
Intestine Antibodies	>9 publications with PON2 and Intestine
Muscle Antibodies	>7 publications with PON2 and Muscle
Placenta Antibodies	>7 publications with PON2 and Placenta