PPAN siRNA – PPAN-P2RY11 Protein Isoform 1 (Human)

Name: PPAN siRNA; PPAN-P2RY11 Protein Isoform 1; Human; MyBioSource
Brand: MyBioSource
SKU: MBS829646
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS829646

MBS829646 is an siRNA targeting PPAN. Also known as PPAN siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit PPAN expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PPAN, siRNA

Full Product Name

PPAN siRNA (Human)

Product Gene Name

PPAN sirna

Product Synonym Names

BXDC3; SSF1; Suppressor of SWI4 1 homolog; Ssf-1; Brix domain-containing protein 3; Peter Pan homolog

Host

Synthetic

Species Reactivity

Human

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

PPAN siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

58,199 Da

OMIM

607793

3D Structure

ModBase 3D Structure for Q9NQ55

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PPAN gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for PPAN sirna

RNA Interference (RNAi)

Other Notes

Small volumes of PPAN sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PPAN. It may not necessarily be applicable to this product.

NCBI GI #

99028871

NCBI GeneID

692312

NCBI Accession #

NP_001035754.1

UniProt Primary Accession #

Q9NQ55

UniProt Secondary Accession #

Q9BW97; Q9H170; C9J3F9

GenBank Nucleotide #

NM_001040664.2

NCBI Official Full Name

PPAN-P2RY11 protein isoform 1

NCBI Official Synonym Full Names

PPAN-P2RY11 readthrough

NCBI Official Symbol

PPAN-P2RY11

UniProt Protein Name

Suppressor of SWI4 1 homolog

UniProt Synonym Protein Names

Brix domain-containing protein 3; Peter Pan homolog

Protein Family

Peter Pan-like protein

UniProt Gene Name

PPAN

UniProt Synonym Gene Names

BXDC3; SSF1; Ssf-1

UniProt Entry Name

SSF1_HUMAN

NCBI Protein Information

PPAN-P2RY11 protein

NCBI Summary for PPAN

This locus represents naturally occurring read-through transcription between the adjacent PPAN and P2RY11 genes. Alternative splicing results in two transcript variants, one of which encodes a fusion protein that shares sequence identity with each individual gene product. This transcript is found to be ubiquitously expressed and is up-regulated by agents inducing granulocytic differentiation. However, its functional significance in vivo remains unclear. [provided by RefSeq, Nov 2010]

UniProt Comments for PPAN

SSF1: May have a role in cell growth. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus; RNA-binding

Chromosomal Location of Human Ortholog: 19p13

Cellular Component: nucleolus; nucleus

Biological Process: RNA splicing

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.