• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

PRDM14 siRNA – Pr Domain Zinc Finger Protein 14 (Human)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8233410

MBS8233410 is an siRNA targeting PRDM14. Also known as PRDM14 siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit PRDM14 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PRDM14, siRNA
Full Product Name
PRDM14 siRNA (Human)
Product Gene Name
PRDM14 sirna
Product Synonym Names
PR domain zinc finger protein 14; PR domain-containing protein 14
Host
Synthetic
Species Reactivity
Human
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
PRDM14 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
64,062 Da
OMIM
611781
3D Structure
ModBase 3D Structure for Q9GZV8
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PRDM14 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for PRDM14 sirna
RNA Interference (RNAi)

Other Notes

Small volumes of PRDM14 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PRDM14. It may not necessarily be applicable to this product.

NCBI GI #
13375636
NCBI GeneID
63978
NCBI Accession #
NP_078780.1
UniProt Primary Accession #
Q9GZV8
UniProt Secondary Accession #
Q86UX9
UniProt Related Accession #
Q9GZV8
GenBank Nucleotide #
NM_024504.3
NCBI Official Full Name
PR domain zinc finger protein 14
NCBI Official Synonym Full Names
PR domain containing 14
NCBI Official Symbol
PRDM14
NCBI Official Synonym Symbols
PFM11
UniProt Protein Name
PR domain zinc finger protein 14
UniProt Synonym Protein Names
PR domain-containing protein 14
Protein Family
PR domain zinc finger protein
UniProt Gene Name
PRDM14
UniProt Entry Name
PRD14_HUMAN
NCBI Protein Information
PR domain zinc finger protein 14

NCBI Summary for PRDM14

This gene encodes a member of the PRDI-BF1 and RIZ homology domain containing (PRDM) family of transcriptional regulators. The encoded protein may possess histone methyltransferase activity and plays a critical role in cell pluripotency by suppressing the expression of differentiation marker genes. Expression of this gene may play a role in breast cancer. [provided by RefSeq, Dec 2011]
Show more

UniProt Comments for PRDM14

PRDM14: a probable protein lysine methyltransferase that works in concert with the core human embryonic stem cell (ESC) regulators to control pluripotency associated genes. Binds to silenced genes in human ESCs. Its binding profile is enriched for the repressive tri-methylation of histone H3 lysine 27 (H3K27me3) modification. Interacts directly with the polycomb repressive complex 2 (PRC2). PRC2 is detected at PRDM14-bound loci in human ESCs. A probable transcriptional regulator of the krueppel C2H2-type zinc-finger protein family. Has both positive and negative roles on transcription. Required for the maintenance of emryonic stem cell identity and the reacquisition of pluripotency in somatic cells. May play an essential role in germ cell development at 2 levels: the reacquisition of potential pluripotency, including SOX2 up-regulation, and successful epigenetic reprogramming. Directly up-regulates the expression of pluripotency gene POU5F1 through its proximal enhancer. Binds to the DNA consensus sequence 5'-GGTC[TC]CTAA-3'.

Protein type: C2H2-type zinc finger protein; Methyltransferase, protein arginine, predicted

Chromosomal Location of Human Ortholog: 8q13.3

Cellular Component: nucleoplasm

Molecular Function: methyltransferase activity; protein binding; DNA binding; metal ion binding

Biological Process: fertilization; transcription, DNA-dependent; regulation of transcription, DNA-dependent; homeostasis of number of cells within a tissue; somatic stem cell maintenance; negative regulation of fibroblast growth factor receptor signaling pathway; inner cell mass cell fate commitment; cell morphogenesis; germ-line stem cell maintenance; cell fate specification; regulation of gene expression, epigenetic; germ cell development
Show more

Research Articles on PRDM14

1. In response to cytokines, pluripotent stem cells differentiate first into a heterogeneous mesoderm-like cell population and then into Primordial Germ cell-Like Cells, which exhibit minimal PRDM14 expression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PRDM14 sirna

 Reference Product  PubMed Publications
 SOX2 sirna  >6 publications with PRDM14 and SOX2
 NANOG sirna  >6 publications with PRDM14 and NANOG
 KLF4 sirna  >3 publications with PRDM14 and KLF4
 POU5F1 sirna  >3 publications with PRDM14 and POU5F1
 SMAD4 sirna  >2 publications with PRDM14 and SMAD4

Pathways associated with PRDM14 sirna

 Products by Pathway  Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Transcriptional Regulation Of Pluripotent Stem Cells Pathway antibodies  Transcriptional Regulation Of Pluripotent Stem Cells Pathway Diagram

Diseases associated with PRDM14 sirna

 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >7 publications with PRDM14 and Breast Neoplasms
 Respiratory Tract Diseases Antibodies  >5 publications with PRDM14 and Respiratory Tract Diseases
 Cell Transformation, Neoplastic Antibodies  >3 publications with PRDM14 and Cell Transformation, Neoplastic
 Neoplasm Metastasis Antibodies  >3 publications with PRDM14 and Neoplasm Metastasis
 Precursor Cell Lymphoblastic Leukemia-Lymphoma Antibodies  >3 publications with PRDM14 and Precursor Cell Lymphoblastic Leukemia-Lymphoma
 Infertility Antibodies  >1 publications with PRDM14 and Infertility
 Neoplasms, Experimental Antibodies  >1 publications with PRDM14 and Neoplasms, Experimental
 Precancerous Conditions Antibodies  >1 publications with PRDM14 and Precancerous Conditions
 Infertility, Male Antibodies  >1 publications with PRDM14 and Infertility, Male
 Testicular Diseases Antibodies  >1 publications with PRDM14 and Testicular Diseases

Organs/Tissues associated with PRDM14 sirna

 Organ/Tissue Name  Pubmed Publications
 Embryonic Tissue Antibodies  >3 publications with PRDM14 and Embryonic Tissue
 Testis Antibodies  >1 publications with PRDM14 and Testis
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000