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SMAD6 siRNA – Mothers Against Decapentaplegic Homolog 6 (Human)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8204769

MBS8204769 is an siRNA targeting SMAD6. Also known as SMAD6 siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit SMAD6 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SMAD6, siRNA
Full Product Name
SMAD6 siRNA (Human)
Product Gene Name
SMAD6 sirna
Product Synonym Names
MADH6; Mothers against decapentaplegic homolog 6; MAD homolog 6; Mothers against DPP homolog 6; SMAD family member 6; SMAD 6; Smad6; hSMAD6
Host
Synthetic
Species Reactivity
Human
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
SMAD6 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
35,467 Da
OMIM
602931
3D Structure
ModBase 3D Structure for O43541
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SMAD6 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for SMAD6 sirna
RNA Interference (RNAi)

Other Notes

Small volumes of SMAD6 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SMAD6. It may not necessarily be applicable to this product.

NCBI GI #
92859871
NCBI GeneID
4091
NCBI Accession #
NP_005576.3
UniProt Primary Accession #
O43541
UniProt Secondary Accession #
O43654; Q15799; Q7Z7L4; Q96E31; Q9UKZ3; A9J6M5
UniProt Related Accession #
O43541
GenBank Nucleotide #
NM_005585.4
NCBI Official Full Name
mothers against decapentaplegic homolog 6
NCBI Official Synonym Full Names
SMAD family member 6
NCBI Official Symbol
SMAD6
NCBI Official Synonym Symbols
AOVD2; MADH6; MADH7; HsT17432
UniProt Protein Name
Mothers against decapentaplegic homolog 6
UniProt Synonym Protein Names
SMAD family member 6; SMAD 6; Smad6; hSMAD6
Protein Family
Mothers against decapentaplegic
UniProt Gene Name
SMAD6
UniProt Synonym Gene Names
MADH6; MAD homolog 6; Mothers against DPP homolog 6; SMAD 6; Smad6; hSMAD6
UniProt Entry Name
SMAD6_HUMAN
NCBI Protein Information
mothers against decapentaplegic homolog 6

NCBI Summary for SMAD6

The protein encoded by this gene belongs to the SMAD family of proteins, which are related to Drosophila 'mothers against decapentaplegic' (Mad) and C. elegans Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions in the negative regulation of BMP and TGF-beta/activin-signalling. Multiple transcript variants have been found for this gene.[provided by RefSeq, Sep 2014]
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UniProt Comments for SMAD6

SMAD6: Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit selectively BMP (bone morphogenetic proteins) signaling by competing with the co-SMAD SMAD4 for receptor-activated SMAD1. SMAD6 is an inhibitory SMAD (I-SMAD) or antagonistic SMAD. Binds to regulatory elements in target promoter regions. Interacts with NEDD4L. Interacts with WWP1. Interacts with RNF111 and AXIN1. Interacts with TGF-beta type I receptor superfamily members, SMAD1, HOXC8 and HOXC9. Interacts with STAMBP and PRKX. Ubiquitous in various organs, with higher levels in lung. Isoform B is up-regulated in diseased heart tissue. Belongs to the dwarfin/SMAD family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 15q22.31

Cellular Component: transcription factor complex; protein complex; cytoplasm; nucleus; cytosol

Molecular Function: identical protein binding; protein binding; ubiquitin protein ligase binding; metal ion binding; transforming growth factor beta receptor, inhibitory cytoplasmic mediator activity; chromatin binding; transcription factor activity

Biological Process: fat cell differentiation; BMP signaling pathway; negative regulation of cell proliferation; zygotic determination of dorsal/ventral axis; transcription, DNA-dependent; ureteric bud development; response to estrogen stimulus; transforming growth factor beta receptor signaling pathway; immune response; negative regulation of transforming growth factor beta receptor signaling pathway; cell-substrate adhesion; negative regulation of apoptosis; negative regulation of BMP signaling pathway

Disease: Aortic Valve Disease 2
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Research Articles on SMAD6

1. Single Nucleotide Polymorphisms in SMAD6 gene is associated with brain metastasis in non-small-cell lung cancer

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with SMAD6 sirna

 Reference Product  PubMed Publications
 SMAD1 sirna  >127 publications with SMAD6 and SMAD1
 BMP2 sirna  >60 publications with SMAD6 and BMP2
 BMPR2 sirna  >28 publications with SMAD6 and BMPR2
 BMP7 sirna  >25 publications with SMAD6 and BMP7
 SMURF1 sirna  >16 publications with SMAD6 and SMURF1
 BMPR1A sirna  >13 publications with SMAD6 and BMPR1A
 BMPR1B sirna  >5 publications with SMAD6 and BMPR1B
 TGFBR1 sirna  >2 publications with SMAD6 and TGFBR1

Pathways associated with SMAD6 sirna

 Products by Pathway  Pathway Diagram
 BMP Receptor Signaling Pathway antibodies  BMP Receptor Signaling Pathway Diagram
 BMP Signalling And Regulation Pathway antibodies  BMP Signalling And Regulation Pathway Diagram
 Integrated Breast Cancer Pathway antibodies  Integrated Breast Cancer Pathway Diagram
 Integrated Pancreatic Cancer Pathway antibodies  Integrated Pancreatic Cancer Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By BMP Pathway antibodies  Signaling By BMP Pathway Diagram
 TGF Beta Signaling Pathway antibodies  TGF Beta Signaling Pathway Diagram
 TGF-beta Receptor Signaling Pathway antibodies  TGF-beta Receptor Signaling Pathway Diagram
 TGF-beta Signaling Pathway antibodies  TGF-beta Signaling Pathway Diagram
 TGF-beta Signaling Pathway antibodies  TGF-beta Signaling Pathway Diagram

Diseases associated with SMAD6 sirna

 Disease Name  Pubmed Publications
 Fibrosis Antibodies  >30 publications with SMAD6 and Fibrosis
 Lung Diseases Antibodies  >18 publications with SMAD6 and Lung Diseases
 Disease Models, Animal Antibodies  >16 publications with SMAD6 and Disease Models, Animal
 Cardiovascular Diseases Antibodies  >15 publications with SMAD6 and Cardiovascular Diseases
 Inflammation Antibodies  >15 publications with SMAD6 and Inflammation
 Hypertension Antibodies  >13 publications with SMAD6 and Hypertension
 Kidney Diseases Antibodies  >12 publications with SMAD6 and Kidney Diseases
 Necrosis Antibodies  >9 publications with SMAD6 and Necrosis
 Heart Diseases Antibodies  >8 publications with SMAD6 and Heart Diseases
 Hypertrophy Antibodies  >7 publications with SMAD6 and Hypertrophy

Organs/Tissues associated with SMAD6 sirna

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >226 publications with SMAD6 and Bone
 Embryonic Tissue Antibodies  >32 publications with SMAD6 and Embryonic Tissue
 Kidney Antibodies  >23 publications with SMAD6 and Kidney
 Heart Antibodies  >21 publications with SMAD6 and Heart
 Connective Tissue Antibodies  >17 publications with SMAD6 and Connective Tissue
 Lung Antibodies  >17 publications with SMAD6 and Lung
 Brain Antibodies  >15 publications with SMAD6 and Brain
 Ovary Antibodies  >12 publications with SMAD6 and Ovary
 Parathyroid Antibodies  >8 publications with SMAD6 and Parathyroid
 Eye Antibodies  >8 publications with SMAD6 and Eye
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