SPTB siRNA – Spectrin Beta Chain, Erythrocytic Isoform B (Human)

Name: SPTB siRNA; Human; MyBioSource
Brand: MyBioSource
SKU: MBS828052
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS828052

MBS828052 is an siRNA targeting SPTB. Also known as SPTB siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit SPTB expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

SPTB, siRNA

Full Product Name

SPTB siRNA (Human)

Product Gene Name

SPTB sirna

Product Synonym Names

SPTB1; Spectrin beta chain. erythrocytic; Beta-I spectrin

Host

Synthetic

Species Reactivity

Human

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

SPTB siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

242,802 Da

OMIM

130600

3D Structure

ModBase 3D Structure for P11277

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SPTB gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for SPTB sirna

RNA Interference (RNAi)

Other Notes

Small volumes of SPTB sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SPTB. It may not necessarily be applicable to this product.

NCBI GI #

67782319

NCBI GeneID

6710

NCBI Accession #

NP_000338.3

UniProt Primary Accession #

P11277

UniProt Secondary Accession #

Q15510; Q15519

UniProt Related Accession #

P11277

GenBank Nucleotide #

NM_000347.5

NCBI Official Full Name

spectrin beta chain, erythrocytic isoform b

NCBI Official Synonym Full Names

spectrin, beta, erythrocytic

NCBI Official Symbol

SPTB

NCBI Official Synonym Symbols

EL3; HS2; SPH2; HSPTB1

UniProt Protein Name

Spectrin beta chain, erythrocytic

UniProt Synonym Protein Names

Beta-I spectrin

Protein Family

Spectrin

UniProt Gene Name

SPTB

UniProt Synonym Gene Names

SPTB1

UniProt Entry Name

SPTB1_HUMAN

NCBI Protein Information

spectrin beta chain, erythrocytic

NCBI Summary for SPTB

This locus encodes a member of the spectrin gene family. Spectrin proteins, along with ankyrin, play a role in cell membrane organization and stability. The protein encoded by this locus functions in stability of erythrocyte membranes, and mutations in this gene have been associated with spherocytosis type 2, hereditary elliptocytosis, and neonatal hemolytic anemia. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2009]

UniProt Comments for SPTB

SPTB1: Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane. Defects in SPTB are the cause of elliptocytosis type 3 (EL3). EL3 is a Rhesus-unlinked form of hereditary elliptocytosis, a genetically heterogeneous, autosomal dominant hematologic disorder. It is characterized by variable hemolytic anemia and elliptical or oval red cell shape. Defects in SPTB are the cause of spherocytosis type 2 (SPH2); also known as hereditary spherocytosis type 2 (HS2). Spherocytosis is a hematologic disorder leading to chronic hemolytic anemia and characterized by numerous abnormally shaped erythrocytes which are generally spheroidal. SPH2 is characterized by severe hemolytic anemia. Inheritance is autosomal dominant. Belongs to the spectrin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 14q23-q24.2

Cellular Component: spectrin; protein complex; cell surface; cytoplasm; cytosol; actin cytoskeleton

Molecular Function: actin filament binding; protein binding; structural constituent of cytoskeleton; protein heterodimerization activity; ankyrin binding; actin binding

Biological Process: axon guidance; plasma membrane organization and biogenesis; actin filament capping; hemopoiesis; porphyrin biosynthetic process

Disease: Spherocytosis, Type 2, Included

Research Articles on SPTB

1. Variations in both alpha-spectrin (SPTA1) and beta-spectrin ( SPTB ) were found in a neonate with prolonged jaundice in a family where nine individuals had hereditary elliptocytosis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with SPTB sirna

Reference Product	PubMed Publications
ANK1 sirna	>10 publications with SPTB and ANK1
SPTA1 sirna	>9 publications with SPTB and SPTA1

Pathways associated with SPTB sirna

Products by Pathway	Pathway Diagram
ARMS-mediated Activation Pathway antibodies	ARMS-mediated Activation Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Cytokine Signaling In Immune System Pathway antibodies	Cytokine Signaling In Immune System Pathway Diagram
DAP12 Interactions Pathway antibodies	DAP12 Interactions Pathway Diagram
DAP12 Signaling Pathway antibodies	DAP12 Signaling Pathway Diagram
Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram
Downstream Signal Transduction Pathway antibodies	Downstream Signal Transduction Pathway Diagram
Downstream Signaling Of Activated FGFR1 Pathway antibodies	Downstream Signaling Of Activated FGFR1 Pathway Diagram
Downstream Signaling Of Activated FGFR2 Pathway antibodies	Downstream Signaling Of Activated FGFR2 Pathway Diagram
Downstream Signaling Of Activated FGFR3 Pathway antibodies	Downstream Signaling Of Activated FGFR3 Pathway Diagram

Diseases associated with SPTB sirna

Disease Name	Pubmed Publications
Inflammation Antibodies	>21 publications with SPTB and Inflammation
Spherocytosis, Hereditary Antibodies	>16 publications with SPTB and Spherocytosis, Hereditary
Anemia, Hemolytic Antibodies	>15 publications with SPTB and Anemia, Hemolytic
Elliptocytosis, Hereditary Antibodies	>7 publications with SPTB and Elliptocytosis, Hereditary
Hemolysis Antibodies	>4 publications with SPTB and Hemolysis
Necrosis Antibodies	>4 publications with SPTB and Necrosis
Edema Antibodies	>2 publications with SPTB and Edema
Learning Disorders Antibodies	>2 publications with SPTB and Learning Disorders
Weight Gain Antibodies	>2 publications with SPTB and Weight Gain
Disease Models, Animal Antibodies	>2 publications with SPTB and Disease Models, Animal

Organs/Tissues associated with SPTB sirna

Organ/Tissue Name	Pubmed Publications
Uterus Antibodies	>16 publications with SPTB and Uterus
Placenta Antibodies	>13 publications with SPTB and Placenta
Embryonic Tissue Antibodies	>3 publications with SPTB and Embryonic Tissue
Nerve Antibodies	>2 publications with SPTB and Nerve
Heart Antibodies	>2 publications with SPTB and Heart
Lung Antibodies	>2 publications with SPTB and Lung
Muscle Antibodies	>2 publications with SPTB and Muscle
Spleen Antibodies	>2 publications with SPTB and Spleen
Brain Antibodies	>2 publications with SPTB and Brain
Mouth Antibodies	>1 publications with SPTB and Mouth