TARS2 siRNA – Threonine--Trna Ligase, Mitochondrial Isoform C (Human)

Name: TARS2 siRNA; Human; MyBioSource
Brand: MyBioSource
SKU: MBS8208856
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8208856

MBS8208856 is an siRNA targeting TARS2. Also known as TARS2 siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit TARS2 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

TARS2, siRNA

Full Product Name

TARS2 siRNA (Human)

Product Gene Name

TARS2 sirna

Product Synonym Names

TARSL1; Threonine--tRNA ligase mitochondrial; Threonyl-tRNA synthetase; ThrRS; Threonyl-tRNA synthetase-like 1

Host

Synthetic

Species Reactivity

Human

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

TARS2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

66,372 Da

OMIM

612805

3D Structure

ModBase 3D Structure for Q9BW92

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TARS2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for TARS2 sirna

RNA Interference (RNAi)

Other Notes

Small volumes of TARS2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TARS2. It may not necessarily be applicable to this product.

NCBI GI #

429535839

NCBI GeneID

80222

NCBI Accession #

NP_001258825.1

UniProt Primary Accession #

Q9BW92

UniProt Secondary Accession #

Q53GW7; Q96I50; Q9H9V2

UniProt Related Accession #

Q9BW92

GenBank Nucleotide #

NM_001271896.1

NCBI Official Full Name

threonine--tRNA ligase, mitochondrial isoform c

NCBI Official Synonym Full Names

threonyl-tRNA synthetase 2, mitochondrial (putative)

NCBI Official Symbol

TARS2

NCBI Official Synonym Symbols

thrRS; TARSL1; COXPD21

UniProt Protein Name

Threonine--tRNA ligase, mitochondrial

UniProt Synonym Protein Names

Threonyl-tRNA synthetase; ThrRS; Threonyl-tRNA synthetase-like 1

UniProt Gene Name

TARS2

UniProt Synonym Gene Names

TARSL1; ThrRS

UniProt Entry Name

SYTM_HUMAN

NCBI Protein Information

threonine--tRNA ligase, mitochondrial

NCBI Summary for TARS2

This gene encodes a member of the class-II aminoacyl-tRNA synthetase family. The encoded protein is a mitochondrial aminoacyl-tRNA synthetase. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 4. [provided by RefSeq, Dec 2012]

UniProt Comments for TARS2

TARS2: Belongs to the class-II aminoacyl-tRNA synthetase family.

Protein type: Ligase; EC 6.1.1.3; Mitochondrial

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: mitochondrial matrix

Molecular Function: threonine-tRNA ligase activity; ATP binding

Biological Process: tRNA aminoacylation for protein translation; gene expression

Disease: Combined Oxidative Phosphorylation Deficiency 21

Research Articles on TARS2

1. TARS2 mutations caused fatal mitochondrial encephalomyopathy in two siblings.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with TARS2 sirna

Products by Pathway	Pathway Diagram
Aminoacyl-tRNA Biosynthesis Pathway antibodies	Aminoacyl-tRNA Biosynthesis Pathway Diagram
Aminoacyl-tRNA Biosynthesis Pathway antibodies	Aminoacyl-tRNA Biosynthesis Pathway Diagram
Aminoacyl-tRNA Biosynthesis, Eukaryotes Pathway antibodies	Aminoacyl-tRNA Biosynthesis, Eukaryotes Pathway Diagram
Aminoacyl-tRNA Biosynthesis, Eukaryotes Pathway antibodies	Aminoacyl-tRNA Biosynthesis, Eukaryotes Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
Mitochondrial TRNA Aminoacylation Pathway antibodies	Mitochondrial TRNA Aminoacylation Pathway Diagram
TRNA Aminoacylation Pathway antibodies	TRNA Aminoacylation Pathway Diagram

Diseases associated with TARS2 sirna

Disease Name	Pubmed Publications
Metabolic Diseases Antibodies	>1 publications with TARS2 and Metabolic Diseases
Brain Diseases Antibodies	>1 publications with TARS2 and Brain Diseases

Organs/Tissues associated with TARS2 sirna

Organ/Tissue Name	Pubmed Publications
Eye Antibodies	>1 publications with TARS2 and Eye