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ZFP36L2 siRNA – Zinc Finger Protein 36, C3H1 Type-Like 2 (Human)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8200076

MBS8200076 is an siRNA targeting ZFP36L2. Also known as ZFP36L2 siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit ZFP36L2 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ZFP36L2, siRNA
Full Product Name
ZFP36L2 siRNA (Human)
Product Gene Name
Product Synonym Names
BRF2; ERF2; RNF162C; TIS11D; Zinc finger protein 36. C3H1 type-like 2; ZFP36-like 2; Butyrate response factor 2; EGF-response factor 2; ERF-2; Protein TIS11D
Host
Synthetic
Species Reactivity
Human
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
ZFP36L2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
51,063 Da
OMIM
612053
3D Structure
ModBase 3D Structure for P47974
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ZFP36L2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for ZFP36L2 sirna
RNA Interference (RNAi)

Other Notes

Small volumes of ZFP36L2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ZFP36L2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
zinc finger protein 36, C3H1 type-like 2
NCBI Official Synonym Full Names
ZFP36 ring finger protein-like 2
NCBI Official Symbol
ZFP36L2
NCBI Official Synonym Symbols
BRF2; ERF2; ERF-2; TIS11D; RNF162C
UniProt Protein Name
Zinc finger protein 36, C3H1 type-like 2
UniProt Synonym Protein Names
Butyrate response factor 2; EGF-response factor 2; ERF-2; Protein TIS11D
UniProt Gene Name
ZFP36L2
UniProt Synonym Gene Names
BRF2; ERF2; RNF162C; TIS11D; ZFP36-like 2; ERF-2
UniProt Entry Name
TISD_HUMAN
NCBI Protein Information
zinc finger protein 36, C3H1 type-like 2

NCBI Summary for ZFP36L2

This gene is a member of the TIS11 family of early response genes. Family members are induced by various agonists such as the phorbol ester TPA and the polypeptide mitogen EGF. The encoded protein contains a distinguishing putative zinc finger domain with a repeating cys-his motif. This putative nuclear transcription factor most likely functions in regulating the response to growth factors. [provided by RefSeq, Jul 2008]
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UniProt Comments for ZFP36L2

ZFP36L2: Probable regulatory protein involved in regulating the response to growth factors. RNA-binding protein that binds to 5'UUAUUUAUUU-3' core sequence. Binds to the class II AU-rich element (ARE) in the 3'-UTR of target mRNAs and promotes their deadenylation and degradation.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 2p22.3-p21

Cellular Component: cytoplasm; nucleus

Molecular Function: DNA binding; metal ion binding; AU-rich element binding; transcription factor activity

Biological Process: cell proliferation; regulation of transcription, DNA-dependent; somatic stem cell maintenance; T cell differentiation in the thymus; mRNA catabolic process; somatic stem cell division; hemopoiesis; regulation of mRNA stability; mRNA catabolic process, deadenylation-dependent decay
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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