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LFA-3/CD58 elisa kit :: Human LFA-3 ELISA Kit

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Catalog # MBS2512588
Unit / Price
  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

LFA-3 (LFA-3/CD58), ELISA Kit

Popular Item
Also Known As

Human LFA-3 (Lymphocyte Function Associated Antigen 3) ELISA Kit

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
248
OMIM
153420
3D Structure
ModBase 3D Structure for P19256
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Human LFA-3/CD58. No significant cross-reactivity or interference between Human LFA-3/CD58 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human LFA-3/CD58 and all the analogues, therefore, cross reaction may still exist.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
12.5-800pg/mL
Sensitivity
Min: 7.5pg/mL; Max: 800pg/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of LFA-3/CD58 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forLFA-3/CD58purchase
MBS2512588 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the LFA-3 (LFA-3/CD58) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LFA-3/CD58. The ELISA analytical biochemical technique of the MBS2512588 kit is based on LFA-3/CD58 antibody-LFA-3/CD58 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LFA-3/CD58 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LFA-3/CD58. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
LFA-3/CD58 elisa kit
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of LFA-3/CD58 in human serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human LFA-3/CD58. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human LFA-3/CD58 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LFA-3/CD58, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human LFA-3/CD58. You can calculate the concentration of Human LFA-3/CD58 in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of LFA-3/CD58 elisa kit
LFA-3/CD58 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2512588. Click to request current manual
NCBI/Uniprot data below describe general gene information for LFA-3/CD58. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
27,960 Da
NCBI Official Full Name
lymphocyte function-associated antigen 3 isoform 2
NCBI Official Synonym Full Names
CD58 molecule
NCBI Official Symbol
NCBI Official Synonym Symbols
ag3; LFA3; LFA-3
  [Similar Products]
NCBI Protein Information
lymphocyte function-associated antigen 3; CD58 antigen, (lymphocyte function-associated antigen 3); surface glycoprotein LFA-3
UniProt Protein Name
Lymphocyte function-associated antigen 3
UniProt Synonym Protein Names
Surface glycoprotein LFA-3; CD_antigen: CD58
UniProt Gene Name
UniProt Synonym Gene Names
LFA3; Ag3  [Similar Products]
UniProt Entry Name
LFA3_HUMAN
NCBI Summary for LFA-3/CD58
This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a ligand of the T lymphocyte CD2 protein, and functions in adhesion and activation of T lymphocytes. The protein is localized to the plasma membrane. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jan 2009]
UniProt Comments for LFA-3/CD58
CD58: Ligand of the T-lymphocyte CD2 glycoprotein. This interaction is important in mediating thymocyte interactions with thymic epithelial cells, antigen-independent and -dependent interactions of T-lymphocytes with target cells and antigen- presenting cells and the T-lymphocyte rosetting with erythrocytes. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 1p13

Cellular Component: cell surface; membrane; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; receptor binding

Biological Process: heterotypic cell-cell adhesion; cell-cell adhesion; blood coagulation; cell adhesion; leukocyte migration
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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