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MCM3 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6176502

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
MCM3, Monoclonal Antibody
Full Product Name
MCM3 (Minichromosome Maintenance Complex Component 3, HCC5, MGC1157, P1-MCM3, P1.h, RLFB) (FITC)
Product Gene Name
Product Synonym Names
Minichromosome Maintenance Complex Component 3; HCC5; MGC1157; P1-MCM3; P1.h; RLFB
Clonality
Monoclonal
Isotype
IgG2a, lambda
Clone Number
2A11
Host
Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Purity/Purification
Purified
Specificity
Recognizes MCM3.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.

FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
95,908 Da
OMIM
602693
3D Structure
ModBase 3D Structure for P25205
Immunogen
MCM3 (NP_002379, 699aa-808aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
AKDGDSYDPYDFSDTEEEMPQVHTPKTADSQETKESQKVELSESRLKAFKVALLDVFREAHAQSIGMNRLTESINRDSEEPFSSVEIQAALSKMQDDNQVMVSEGIIFLI
Conjugate
FITC

Product Categories/Family for anti-MCM3 antibody

Applications Tested/Suitable for anti-MCM3 antibody
Western Blot (WB)
Application Notes for anti-MCM3 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-MCM3 antibody

MCM3 monoclonal antibody (M02), clone 2A11 Western Blot analysis of MCM3 expression in Hela S3 NE (Cat # L013V3).
Mouse Anti-MCM3 Monoclonal Antibody – Cat# MBS6176502

Testing Data of anti-MCM3 antibody

Detection limit for recombinant GST tagged MCM3 is approximately 0.1ng/ml as a capture antibody.
Mouse Anti-MCM3 Monoclonal Antibody, Testing Data – Cat# MBS6176502

Other Notes

Small volumes of anti-MCM3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MCM3 purchase

MBS6176502 is an antibody targeting MCM3. Also known as MCM3 (Minichromosome Maintenance Complex Component 3, HCC5, MGC1157, P1-MCM3, P1.h, RLFB) (FITC). Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for MCM3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
DNA replication licensing factor MCM3 isoform 1
NCBI Official Synonym Full Names
minichromosome maintenance complex component 3
NCBI Official Symbol
MCM3
NCBI Official Synonym Symbols
HCC5; P1.h; RLFB; P1-MCM3
UniProt Protein Name
DNA replication licensing factor MCM3
UniProt Synonym Protein Names
DNA polymerase alpha holoenzyme-associated protein P1; P1-MCM3; RLF subunit beta; p102
UniProt Gene Name
MCM3
UniProt Entry Name
MCM3_HUMAN
NCBI Protein Information
DNA replication licensing factor MCM3; DNA polymerase alpha holoenzyme-associated protein P1; DNA replication factor MCM3; MCM3 minichromosome maintenance deficient 3; RLF subunit beta; cervical cancer proto-oncogene 5; hRlf beta subunit; p102; replicatio

UniProt Comments for MCM3

MCM3: a mini-chromosome maintenance protein, essential for the initiation of eukaryotic genome replication. Allows DNA to undergo a single round of replication per cell cycle. Required for DNA replication and cell proliferation.

Protein type: EC 3.6.4.12; DNA replication

Chromosomal Location of Human Ortholog: 6p12

Cellular Component: nucleoplasm; centrosome; alpha DNA polymerase:primase complex; membrane; intracellular membrane-bound organelle; MCM complex; perinuclear region of cytoplasm; nucleus

Molecular Function: DNA helicase activity; protein binding; DNA binding; ATP binding

Biological Process: DNA replication initiation; mitotic cell cycle; DNA strand elongation during DNA replication; DNA replication; DNA duplex unwinding; G1/S transition of mitotic cell cycle
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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