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V5 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8100287

The V5 epitope tag has a 14-amino-acid sequence (GKPIPNPLLGLDST) which is derived from simian parainfluenza virus type 5 (SV5). The V5 epitope tag is used to label a protein of interest so that the tagged protein can be analyzed and visualized. Anti-V5… More details

$195 / 0.02 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
V5, Monoclonal Antibody
Full Product Name
Anti-V5 tag Antibody, Mouse Monoclonal
Product Gene Name
anti-V5 antibody
Product Synonym Names
Anti-V5 Tag Antibody, Mouse Mab
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
03
Host
Mouse
Species Reactivity
V5 tag
Form/Format
Liquid; 0.2um filtered solution in PBS
Purity/Purification
Protein A
Specificity
Recognize N-terminal and C-terminal V5 Tag in fusion proteins
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunogen
A synthetic peptide corresponding to the V5 tag sequence (GKPIPNPLLGLDST)
Conjugation
Unconjugated
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the V5 tag sequence (GKPIPNPLLGLDST). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Applications Tested/Suitable for anti-V5 antibody
Western Blot (WB)
Application Notes for anti-V5 antibody
WB: 1:500-1:2000

Western Blot (WB) of anti-V5 antibody

Anti-V5 Tag mouse monoclonal antibody at 1:500 dilution.
Lane A: his-ARG1-V5 transfected 293 cell Lysate
(60ug)
Lane B: mFABP4-V5-his transfected 293 cell Lysate
(60ug)
Lane C: V5-ARG1-his transfected 293 cell Lysate
(10ug)
Lane D: V5-mFABP4-his transfected 293 cell Lysate
(20ug)
Lane E: Negative control transfected 293 cell Lysate
(30ug)
Secondary
Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.
Developed using the Odyssey technique.
Performed under reducing conditions.
Mouse Anti-V5 Monoclonal Antibody – Cat# MBS8100287

Product References and Citations for anti-V5 antibody

Zhao X, et al. (2013) Several affinity tags commonly used in chromatographic purification. J Anal Methods Chem 2013 581093.Monti M, et al. (2007) Functional proteomics: Protein-protein interactions in vivo. Ital J Biochem 56 (4): 310-314.Brothers SP, et al. (2003) Unexpected effects of epitope and chimeric tags on gonadotropin-releasing hormone receptors: Implications for understanding the molecular etiology of hypogonadotropic hypogonadism. J Clin Endocrinol Metab 88 (12): 6107-6112.

Other Notes

Small volumes of anti-V5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for V5 purchase

MBS8100287 is an antibody targeting V5. Also known as Anti-V5 tag Antibody, Mouse Monoclonal. Reactive in V5 tag. Host: Mouse.
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