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CD133 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9601293

Description: CD133, originally known as AC133. CD133 is a glycoprotein also known in humans and rodents as Prominin 1 (PROM1). Currently the function of CD133 is unknown. It is a member of pentaspan transmembrane glycoproteins (5-transmembrane, 5-TM), which… More details

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Product Overview

Online Datasheet in PDF
Product Name
CD133, Polyclonal Antibody
Full Product Name
CD133 Antibody
Product Gene Name
anti-CD133 antibody
Product Synonym Gene Name
PROM1
Product Synonym Names
AC133; Antigen AC133; CD133; CORD12; Hematopoietic stem cell antigen; hProminin; MCDR2; MSTP061; OTTHUMP00000217744; OTTHUMP00000217745; OTTHUMP00000217746; PROM1; PROM1_HUMAN; Prominin I; Prominin like 1; Prominin like protein 1 precursor; Prominin mouse like 1; Prominin-1; Prominin-like protein 1; Prominin1; PROML1; RP41; STGD4
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Concentration
1mg/ml (lot specific)
Specificity
CD133 antibody detects endogenous levels of total CD133
Preparation and Storage
Store at -20°C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
CD133 peptide (MBS9616825) is used for blocking the activity of CD133 antibody (MBS9601293)
Molecular Weight
97 kDa
3D Structure
ModBase 3D Structure for O43490
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human CD133, corresponding to a region within N-terminal amino acids.
Conjugation
Unconjugated
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-CD133 antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-CD133 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
IF/ICC: 1:200

The optimal dilutions to be determined by the end user.

IMPORTANT:
For western blot, incubate membrane with diluted primary Abin 5% w/v milk , 1X TBS, 0.1% Tween20 at 4°C with gentle shaking, overnight.

Immunofluorescence (IF) of anti-CD133 antibody

MBS9601293 staining Caco 2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
Rabbit Anti-CD133 Polyclonal Antibody – Cat# MBS9601293

Western Blot (WB) of anti-CD133 antibody

Western blot analysis of CD133 expression in SW620 cells, The lane on the left is treated with the antigen-specific peptide.
Rabbit Anti-CD133 Polyclonal Antibody, Western Blot (WB) – Cat# MBS9601293

Immunohistochemistry (IHC) of anti-CD133 antibody

CD133 Antibody for IHC in human testis.
Rabbit Anti-CD133 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9601293

Western Blot of anti-CD133 antibody

Western blot analysis of extracts from 293, using CD133 Antibody. The lane on the left was treated with blockingpeptide.
Rabbit Anti-CD133 Polyclonal Antibody, Western Blot – Cat# MBS9601293

Western Blot (WB) of anti-CD133 antibody

Western blot analysis of lysates from PC12 cells(LPS,4h treatment), using CD133 Ab at 1/1000 dilution.
5ug/NC membrane strip.
Exposure for 10s with Affinity ECL Kit.
Bands result from membrane strip incubation
Rabbit Anti-CD133 Polyclonal Antibody, Western Blot (WB) – Cat# MBS9601293

Other Notes

Small volumes of anti-CD133 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD133 purchase

MBS9601293 is an antibody targeting CD133. Also known as CD133 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CD133. It may not necessarily be applicable to this product.

NCBI GI #
224994189
NCBI GeneID
8842
NCBI Accession #
NP_001139319.1
UniProt Primary Accession #
O43490
UniProt Secondary Accession #
Q6SV49; Q6SV50; Q6SV51; Q6SV52; Q6SV53; Q96EN6
UniProt Related Accession #
O43490
GenBank Nucleotide #
NM_001145847.1
NCBI Official Full Name
prominin-1 isoform 2
NCBI Official Synonym Full Names
prominin 1
NCBI Official Symbol
PROM1
NCBI Official Synonym Symbols
RP41; AC133; CD133; MCDR2; STGD4; CORD12; PROML1; MSTP061
UniProt Protein Name
Prominin-1
UniProt Synonym Protein Names
Antigen AC133; Prominin-like protein 1; CD_antigen: CD133
Protein Family
Prominin
UniProt Gene Name
PROM1
UniProt Synonym Gene Names
PROML1
NCBI Protein Information
prominin-1

NCBI Summary for CD133

This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
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UniProt Comments for CD133

May play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617). Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed:20818439).
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Research Articles on CD133

1. The expression of CD133 protein in patients with osteosarcoma was significantly high and related to the distant metastasis, which may be a potential prediction biomarker for poor prognosis of osteosarcoma patients

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD133 antibody

 Reference Product  PubMed Publications
 CD44 antibody  >8 publications with CD133 and CD44
 CD34 antibody  >3 publications with CD133 and CD34
 KDR antibody  >2 publications with CD133 and KDR
 HIF1A antibody  >2 publications with CD133 and HIF1A
 CDHR1 antibody  >2 publications with CD133 and CDHR1
 NES antibody  >1 publications with CD133 and NES
 RET antibody  >1 publications with CD133 and RET

Pathways associated with anti-CD133 antibody

 Products by Pathway  Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram

Diseases associated with anti-CD133 antibody

 Disease Name  Pubmed Publications
 Colorectal Neoplasms Antibodies  >12 publications with CD133 and Colorectal Neoplasms
 Carcinoma Antibodies  >9 publications with CD133 and Carcinoma
 Adenocarcinoma Antibodies  >6 publications with CD133 and Adenocarcinoma
 Glioblastoma Antibodies  >6 publications with CD133 and Glioblastoma
 Colonic Neoplasms Antibodies  >5 publications with CD133 and Colonic Neoplasms
 Disease Models, Animal Antibodies  >5 publications with CD133 and Disease Models, Animal
 Neoplasm Metastasis Antibodies  >4 publications with CD133 and Neoplasm Metastasis
 Liver Neoplasms Antibodies  >3 publications with CD133 and Liver Neoplasms
 Liver Diseases Antibodies  >3 publications with CD133 and Liver Diseases
 Cell Transformation, Neoplastic Antibodies  >3 publications with CD133 and Cell Transformation, Neoplastic

Organs/Tissues associated with anti-CD133 antibody

 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >16 publications with CD133 and Eye
 Brain Antibodies  >14 publications with CD133 and Brain
 Liver Antibodies  >10 publications with CD133 and Liver
 Nerve Antibodies  >5 publications with CD133 and Nerve
 Intestine Antibodies  >4 publications with CD133 and Intestine
 Embryonic Tissue Antibodies  >4 publications with CD133 and Embryonic Tissue
 Lung Antibodies  >3 publications with CD133 and Lung
 Stomach Antibodies  >2 publications with CD133 and Stomach
 Testis Antibodies  >2 publications with CD133 and Testis
 Mammary Gland Antibodies  >1 publications with CD133 and Mammary Gland
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