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BLK Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9524721

This gene encodes a nonreceptor tyrosine-kinase of the src family of proto-oncogenes that are typically involved in cell proliferation and differentiation. The protein has a role in B-cell receptor signaling and B-cell development. The protein also stimulates… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Blk, Monoclonal Antibody
Full Product Name
Blk Monoclonal Antibody
Product Gene Name
Product Synonym Names
Tyrosine-protein kinase Blk; BLK; Tyrosine-protein kinase Blk; B lymphocyte kinase; p55-Blk
Clonality
Monoclonal
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purity/Purification
Affinity purification
Specificity
Blk Monoclonal Antibody detects endogenous levels of Blk protein.
Preparation and Storage
Store at -20 degree C/1 year
Molecular Weight
57,706 Da
Chromosome Location
Chromosome: 8; NC_000008.10 (11351521..11422108). Location: 8p23-p22
OMIM
191305
3D Structure
ModBase 3D Structure for P51451
Immunogen
Purified recombinant fragment of human Blk expressed in E coli.
Modification
Unmodified/Total

Product Categories/Family for anti-Blk antibody

Applications Tested/Suitable for anti-Blk antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS/FCM), ELISA (EIA)
Application Notes for anti-Blk antibody
WB: 1:500-1:2000
IF: 1:200-1:1000
FC/FACS/FCM: 1:200-1:400
ELISA: 1:10000

Western Blot (WB) of anti-Blk antibody

Western Blot analysis using Blk Monoclonal Antibody against HEK293 (1) and BLK-hIgGFc transfected HEK293 (2) cell lysate.
Mouse Anti-BLK Monoclonal Antibody – Cat# MBS9524721

Immunofluorescence (IF) of anti-Blk antibody

Immunofluorescence analysis of Hela cells using Blk Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Mouse Anti-BLK Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS9524721

Flow Cytometry (FC/FACS) of anti-Blk antibody

Flow cytometric analysis of HL-60 cells using Blk Monoclonal Antibody (green) and negative control (purple).
Mouse Anti-BLK Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS9524721

Other Notes

Small volumes of anti-Blk antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Blk purchase

MBS9524721 is an antibody targeting Blk. Also known as Blk Monoclonal Antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Blk. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
tyrosine-protein kinase Blk
NCBI Official Synonym Full Names
B lymphoid tyrosine kinase
NCBI Official Symbol
BLK
NCBI Official Synonym Symbols
MODY11
UniProt Protein Name
Tyrosine-protein kinase Blk
UniProt Synonym Protein Names
B lymphocyte kinase; p55-Blk
UniProt Gene Name
BLK
UniProt Entry Name
BLK_HUMAN
NCBI Protein Information
tyrosine-protein kinase Blk; p55-Blk; b lymphocyte kinase; BLK nonreceptor tyrosine kinase

UniProt Comments for Blk

BLK: a nonreceptor tyrosine kinase of the Src family. Expressed predominantly in B lymphocytes

Protein type: Protein kinase, TK; Protein kinase, tyrosine (non-receptor); Kinase, protein; EC 2.7.10.2; TK group; Src family

Chromosomal Location of Human Ortholog: 8p23-p22

Cellular Component: extrinsic to internal side of plasma membrane

Molecular Function: protein binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding

Biological Process: B cell receptor signaling pathway; innate immune response; positive regulation of insulin secretion; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of cell proliferation; T cell differentiation

Disease: Maturity-onset Diabetes Of The Young, Type 11
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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