• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

CD55 Recombinant Antibody – Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS488248

Recombinant monoclonal antibody to CD55. Manufactured using Recombinant Platform with variable regions (i.e. specificity) from the scFv LU30. More details

$635 / 0.2 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD55, Recombinant Antibody
Full Product Name
Anti-CD55 [LU30]
Product Gene Name
Product Synonym Names
Complement decay-accelerating factor; DAF
Clonality
Monoclonal
Isotype
Rabbit IgG, lambda
Clone Number
LU30
Species Reactivity
Human
Purity/Purification
Purified
Protein A Affinity Purified
Specificity
The antibody binds to CD55 with a Kd of 13 nM.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Molecular Weight
59,038 Da
OMIM
125240
Animal-free Manufacturing Platform
The recombinant antibody is manufactured using a recombinant, mammalian HEK293-based expression platform. The manufacturing platform is animal-free and animal component-free.
Immunogen
Human CD55.
Antigen
CD55
Antigen Description
Human CD55.
Buffer Composition
PBS only with 0.02% Proclin 300.
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original Human format, for improved compatibility with existing reagents, assays and techniques.
Publication Note
Describes the use of a human single-chainFv phage library to search tumor-associated antigens and the production of monoclonal antibodies against the antigens.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for CD55 recombinant antibody
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)

Flow Cytometry (FC/FACS) of CD55 recombinant antibody

Flow-cytometry using the anti-CD55 (Complement decay-accelerating factor) antibody LU-30 U93 cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of LU-30 at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.
Anti-CD55 Monoclonal Recombinant Antibody – Cat# MBS488248

Immunohistochemistry (IHC) of CD55 recombinant antibody

Immunohistochemical staining of human lung tissue using anti-CD55 antibody LU30 Anti-CD55 (Complement decay-accelerating factor)staining of paraffin embedded human lung tissue using the rabbit-chimeric version of LU30 for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter staining with haematoxylin. Strong staining of the epithelial surface of alveoli may be observed. Recommended concentration, 2-4 ug/ml.
Anti-CD55 Monoclonal Recombinant Antibody, Immunohistochemistry (IHC) – Cat# MBS488248

Product References and Citations for CD55 recombinant antibody

Ridgway,J.B., Ng,E., Kern,J.A., Lee,J., Brush,J., Goddard,A. and Carter,P. Cancer Res. 59 (11),2718-2723 (1999) PMID:10363997

Other Notes

Small volumes of CD55 recombinant antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD55 purchase

MBS488248 is a recombinant antibody targeting CD55. Also known as Anti-CD55 [LU30]. Reactive in Human.

Additional Details

NCBI/Uniprot data below describe general gene information for CD55. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
CD55, partial
NCBI Official Synonym Full Names
CD55 molecule (Cromer blood group)
NCBI Official Symbol
CD55
NCBI Official Synonym Symbols
CR; TC; DAF; CROM; CHAPLE
UniProt Protein Name
Complement decay-accelerating factor
UniProt Synonym Protein Names
CD_antigen: CD55
UniProt Gene Name
CD55
UniProt Synonym Gene Names
CR; DAF
NCBI Protein Information
complement decay-accelerating factor

NCBI Summary for CD55

This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM). Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins. [provided by RefSeq, Jul 2014]
Show more

UniProt Comments for CD55

This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade (PubMed:7525274). Inhibits complement activation by destabilizing and preventing the formation of C3 and C5 convertases, which prevents complement damage (PubMed:28657829).
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000