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Vimentin (VIM) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6134534

MBS6134534 is an antibody targeting VIM. Also known as Vimentin (VIM) (AP). Reactive in Human. Host: Mouse. More details

$995 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Vimentin, Monoclonal Antibody
Full Product Name
Vimentin (VIM) (AP)
Product Gene Name
anti-VIM antibody
Product Synonym Names
FLJ36605
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
3E9
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human VIM.
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunogen
Partial recombinant corresponding to aa41-140 from VIM (AAH30573) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
GSALRPSTSRSLYASSPGGVYATRSSAVRLRSSVPGVRLLQDSVDFSLADAINTEFKNTRTNEKVELQELNDRFANYIDKVRFLEQQNKILLAELEQLKG
Conjugate
AP

Product Categories/Family for anti-VIM antibody

Antibodies; Extracellular Matrix Proteins
Applications Tested/Suitable for anti-VIM antibody
ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-VIM antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-VIM antibody

VIM monoclonal antibody, Western Blot analysis of VIM expression in HeLa.
Mouse Anti-VIM Monoclonal Antibody – Cat# MBS6134534

Western Blot (WB) of anti-VIM antibody

Western Blot analysis of VIM expression in transfected 293T cell line by VIM monoclonal antibody. Lane 1: VIM transfected lysate (53.7kD). Lane 2: Non-transfected lysate.
Mouse Anti-VIM Monoclonal Antibody, Western Blot (WB) – Cat# MBS6134534

Immunofluorescence (IF) of anti-VIM antibody

Immunofluorescence of monoclonal antibody to VIM on HeLa cell. [antibody concentration 40ug/ml]
Mouse Anti-VIM Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6134534

Immunoprecipitation (IP) of anti-VIM antibody

Immunoprecipitation of VIM transfected lysate using VIM monoclonal antibody and Protein A Magnetic Bead and immunoblotted with VIM rabbit polyclonal antibody.
Mouse Anti-VIM Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS6134534

Other Notes

Small volumes of anti-VIM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for VIM. It may not necessarily be applicable to this product.

NCBI GI #
33989649
NCBI GeneID
7431
NCBI Accession #
BC030573
NCBI Related Accession #
AAH30573
UniProt Related Accession #
P08670
NCBI Official Full Name
Homo sapiens vimentin, mRNA
NCBI Official Synonym Full Names
vimentin
NCBI Official Symbol
VIM
Protein Family
Vimentin
NCBI Protein Information
vimentin

NCBI Summary for VIM

This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]
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Research Articles on VIM

1. High vimentin Expression in Tumor Microenvironment is associated with Pancreatic Ductal Adenocarcinoma.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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