Protein SET (SET) ELISA Kit for Mouse

Name: Mouse SET ELISA Kit; Protein SET; MyBioSource
Brand: MyBioSource
SKU: MBS2804953
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2804953

MBS2804953 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Protein SET (SET) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Protein SET (SET), ELISA Kit

Full Product Name

Mouse Protein SET (SET) ELISA Kit

Product Gene Name

SET elisa kit

Product Synonym Names

2PP2A; I2PP2A; IGAAD; IPP2A2; PHAPII; TAF-I; TAF-IBETA; HLA-DR-associated protein II|SET translocation (myeloid leukemia-associated)|Template-Activating Factor-I; chromatin remodelling factor|inhibi

Species Reactivity

Mouse

Assay Type

Quantitive Sandwich

Samples

Serum, Plasma, Other biological fluids

Detection Range

0.156-10ng/mL

Sensitivity

0.078ng/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of Mouse SET. No significant cross-reactivity or interference between Mouse SET and analogues was observed.

Preparation and Storage

Store at 2-8 degree C. Please refer to Instruction Manual.

Molecular Weight

17,461 Da

3D Structure

ModBase 3D Structure for Q9EQU5

Detection Wavelength

450nm

Assay Duration

1-4.5h

Sample Volume

1-200uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV

Test Principle

This assay employs a two-site sandwich ELISA to quantitate SET in samples. An antibody specific for SET has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SET present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SET is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SET bound in the initial step. The color development is stopped and the intensity of the color is measured.

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of SET elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SET. It may not necessarily be applicable to this product.

NCBI GI #

325910859

NCBI GeneID

56086

NCBI Accession #

NP_001191804.1

UniProt Primary Accession #

Q9EQU5

UniProt Secondary Accession #

Q9CY82; Q9D0A9; Q9Z181; A2BE95

UniProt Related Accession #

Q9EQU5

GenBank Nucleotide #

NM_001204875.1

NCBI Official Full Name

protein SET isoform 2

NCBI Official Synonym Full Names

SET nuclear oncogene

NCBI Official Symbol

Set

NCBI Official Synonym Symbols

TAF-I; I-2PP2A; AA407739; StF-IT-1; 2610030F17Rik; 5730420M11Rik

UniProt Protein Name

Protein SET

UniProt Synonym Protein Names

Phosphatase 2A inhibitor I2PP2A; I-2PP2A; Template-activating factor I; TAF-I

Protein Family

Protein

UniProt Gene Name

Set

UniProt Synonym Gene Names

I-2PP2A; TAF-I

UniProt Entry Name

SET_MOUSE

NCBI Protein Information

protein SET

UniProt Comments for SET

SET: a potent inhibitor of protein phosphatase 2A involved in apoptosis, transcription, nucleosome assembly and histone binding. The translocation t(6;9)(q21;q34.1) NUP214/CAN is found in some cases of acute undifferentiated leukemia (AUL). Two alternatively spliced isoforms have been described. Isoform 1 and isoform 2 interact directly with each other and with ANP32A within the tripartite INHAT (inhibitor of acetyltransferases) complex. Both isoforms inhibit the acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition.

Protein type: Inhibitor; DNA replication; Apoptosis; Oncoprotein; Nuclear receptor co-regulator; DNA-binding

Cellular Component: protein complex; endoplasmic reticulum; perinuclear region of cytoplasm; cytoplasm; nucleus

Molecular Function: DNA binding

Biological Process: nucleosome assembly; negative regulation of neuron apoptosis; negative regulation of transcription, DNA-dependent

Research Articles on SET

1. SET protein was expressed in spermatogonia and spermatocytes; the highest level was mainly in haploid and tetraploid cells of the prepubertal and adult groups, and Leydig cells of the adult and ageing groups. There was a low expression in Sertoli cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with SET elisa kit

Reference Product	PubMed Publications
Nup214 elisa kit	>29 publications with SET and Nup214
Anp32a elisa kit	>18 publications with SET and Anp32a
Apex1 elisa kit	>17 publications with SET and Apex1
Elavl1 elisa kit	>12 publications with SET and Elavl1
Xpo1 elisa kit	>6 publications with SET and Xpo1
Tnfsf12 elisa kit	>5 publications with SET and Tnfsf12
Smc2 elisa kit	>2 publications with SET and Smc2
Smc4 elisa kit	>1 publications with SET and Smc4

Pathways associated with SET elisa kit

Products by Pathway	Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle, Mitotic Pathway antibodies	Cell Cycle, Mitotic Pathway Diagram
Condensation Of Prophase Chromosomes Pathway antibodies	Condensation Of Prophase Chromosomes Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
HuR Stabilizes MRNA Pathway antibodies	HuR Stabilizes MRNA Pathway Diagram
M Phase Pathway antibodies	M Phase Pathway Diagram
Mitotic Prophase Pathway antibodies	Mitotic Prophase Pathway Diagram
Regulation Of MRNA Stability By Proteins That Bind AU-rich Elements Pathway antibodies	Regulation Of MRNA Stability By Proteins That Bind AU-rich Elements Pathway Diagram

Diseases associated with SET elisa kit

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>22300 publications with SET and Nervous System Diseases
Cardiovascular Diseases Antibodies	>14336 publications with SET and Cardiovascular Diseases
Immune System Diseases Antibodies	>10722 publications with SET and Immune System Diseases
Death Antibodies	>8906 publications with SET and Death
Heart Diseases Antibodies	>7355 publications with SET and Heart Diseases
Inflammation Antibodies	>4843 publications with SET and Inflammation
Breast Neoplasms Antibodies	>4685 publications with SET and Breast Neoplasms
Congenital Abnormalities Antibodies	>4368 publications with SET and Congenital Abnormalities
Disease Models, Animal Antibodies	>4201 publications with SET and Disease Models, Animal
Liver Diseases Antibodies	>3611 publications with SET and Liver Diseases

Organs/Tissues associated with SET elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>37454 publications with SET and Blood
Brain Antibodies	>24869 publications with SET and Brain
Heart Antibodies	>12727 publications with SET and Heart
Muscle Antibodies	>11245 publications with SET and Muscle
Lung Antibodies	>8218 publications with SET and Lung
Liver Antibodies	>7862 publications with SET and Liver
Joint Antibodies	>7780 publications with SET and Joint
Skin Antibodies	>6953 publications with SET and Skin
Eye Antibodies	>6521 publications with SET and Eye
Kidney Antibodies	>5597 publications with SET and Kidney