Stem Cell Factor (SCF) ELISA Kit for Mouse

Name: Mouse SCF ELISA Kit; Stem Cell Factor; MyBioSource
Brand: MyBioSource
SKU: MBS2089504
Price: 390 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2089504

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Stem Cell Factor (SCF). Standards or samples are then added to the appropriate… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Stem Cell Factor (SCF), ELISA Kit

Full Product Name

Mouse Stem Cell Factor (SCF) Mini Samples ELISA Kit

Product Gene Name

SCF elisa kit

Product Synonym Names

KITLG; MGF; SF; KL1; Kitl; FPH2; Kit-Ligand; Steel Factor; Mast Cell Growth Factor; Steel factor; Familial Progressive Hyperpigmentation 2

Species Reactivity

Mouse

Assay Type

Double-antibody Sandwich

Samples

Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids

Detection Range

15.6-1,000pg/mL

Sensitivity

5.9pg/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of Mini Samples Stem Cell Factor (SCF).
No significant cross-reactivity or interference between Mini Samples Stem Cell Factor (SCF) and analogues was observed.

Preparation and Storage

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Molecular Weight

27,614 Da

3D Structure

ModBase 3D Structure for P20826

Supply Chain Verification

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Intra-assay Precision (Precision within an assay)

3 samples with low, middle and high level Mini Samples Stem Cell Factor (SCF) were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays)

3 samples with low, middle and high level Mini Samples Stem Cell Factor (SCF) were tested on 3 different plates, 8 replicates in each plate.

CV(%) =

SD/meanX100

Intra-Assay

CV<10%

Inter-Assay

CV<12%

Product Categories/Family for SCF elisa kit

Cytokine; Hematology

ISO Certification

Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.

Other Notes

Small volumes of SCF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SCF purchase

MBS2089504 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Stem Cell Factor (SCF) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SCF. The ELISA analytical biochemical technique of the MBS2089504 kit is based on SCF antibody-SCF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SCF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SCF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for SCF. It may not necessarily be applicable to this product.

NCBI GI #

7305267

NCBI GeneID

17311

NCBI Accession #

NP_038626.1

UniProt Primary Accession #

P20826

UniProt Secondary Accession #

P97332; Q3TNJ7; Q62524; Q64222; Q921N5

UniProt Related Accession #

P20826

GenBank Nucleotide #

NM_013598.3

NCBI Official Full Name

kit ligand isoform 1

NCBI Official Synonym Full Names

kit ligand

NCBI Official Symbol

Kitl

NCBI Official Synonym Symbols

Gb; SF; Sl; Clo; Con; Mgf; SCF; SLF; blz; Kitlg; contrasted

UniProt Protein Name

Kit ligand

UniProt Synonym Protein Names

Hematopoietic growth factor KL; Mast cell growth factor; MGF

Protein Family

Kit ligand

UniProt Gene Name

Kitlg

UniProt Synonym Gene Names

Kitl; Mgf; Sl; Slf; MGF; SCF; sKITLG

NCBI Protein Information

kit ligand

UniProt Comments for SCF

SCF: Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5- trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins. Homodimer, non-covalently linked (Probable). Heterotetramer with KIT, binding two KIT molecules; thereby mediates KIT dimerization and subsequent activation by autophosphorylation. Belongs to the SCF family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 10 D1|10 51.4 cM

Cellular Component: cytoplasm; extracellular space; filopodium; integral to membrane; lamellipodium; plasma membrane

Molecular Function: cytokine activity; protein binding; stem cell factor receptor binding

Biological Process: embryonic hemopoiesis; germ cell development; germ cell programmed cell death; negative regulation of apoptosis; negative regulation of mast cell apoptosis; neural crest cell migration; ovarian follicle development; positive regulation of cell proliferation; positive regulation of DNA replication; positive regulation of leukocyte migration; positive regulation of MAP kinase activity; positive regulation of melanocyte differentiation; positive regulation of myeloid leukocyte differentiation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of Ras protein signal transduction

Research Articles on SCF

1. SCF/c-kit signaling may potentiate chronic hypoxia-induced vascular remodeling by modulating ERK activation. Inhibition of c-kit activity may be a potential strategy to alleviate PH

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with SCF elisa kit

Products by Pathway	Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Constitutive PI3K/AKT Signaling In Cancer Pathway antibodies	Constitutive PI3K/AKT Signaling In Cancer Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
DAP12 Interactions Pathway antibodies	DAP12 Interactions Pathway Diagram
DAP12 Signaling Pathway antibodies	DAP12 Signaling Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
Downstream Signal Transduction Pathway antibodies	Downstream Signal Transduction Pathway Diagram
Downstream Signaling Events Of B Cell Receptor (BCR) Pathway antibodies	Downstream Signaling Events Of B Cell Receptor (BCR) Pathway Diagram
Downstream Signaling Of Activated FGFR Pathway antibodies	Downstream Signaling Of Activated FGFR Pathway Diagram

Diseases associated with SCF elisa kit

Disease Name	Pubmed Publications
Disease Models, Animal Antibodies	>10 publications with SCF and Disease Models, Animal
Neoplasms, Experimental Antibodies	>6 publications with SCF and Neoplasms, Experimental
Inflammation Antibodies	>5 publications with SCF and Inflammation
Weight Loss Antibodies	>3 publications with SCF and Weight Loss
Fibrosis Antibodies	>3 publications with SCF and Fibrosis
Metaplasia Antibodies	>3 publications with SCF and Metaplasia
Infertility, Male Antibodies	>3 publications with SCF and Infertility, Male
Lung Diseases Antibodies	>3 publications with SCF and Lung Diseases
Necrosis Antibodies	>3 publications with SCF and Necrosis
Liver Diseases Antibodies	>2 publications with SCF and Liver Diseases

Organs/Tissues associated with SCF elisa kit

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>31 publications with SCF and Bone
Ovary Antibodies	>26 publications with SCF and Ovary
Skin Antibodies	>21 publications with SCF and Skin
Bone Marrow Antibodies	>20 publications with SCF and Bone Marrow
Testis Antibodies	>18 publications with SCF and Testis
Embryonic Tissue Antibodies	>9 publications with SCF and Embryonic Tissue
Liver Antibodies	>8 publications with SCF and Liver
Heart Antibodies	>6 publications with SCF and Heart
Muscle Antibodies	>6 publications with SCF and Muscle
Lung Antibodies	>5 publications with SCF and Lung