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ATP6AP1 siRNA – V-Type Proton Atpase Subunit S1 (Mouse)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8222343

MBS8222343 is an siRNA targeting ATP6AP1. Also known as ATP6AP1 siRNA (Mouse). Reactive in Mouse. Host: Synthetic. siRNA to inhibit ATP6AP1 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ATP6AP1, siRNA
Full Product Name
ATP6AP1 siRNA (Mouse)
Product Gene Name
ATP6AP1 sirna
Product Synonym Names
ATP6IP1; ATP6S1; V-type proton ATPase subunit S1; V-ATPase subunit S1; Protein C7-1; V-ATPase Ac45 subunit; V-ATPase S1 accessory protein; Vacuolar proton pump subunit S1
Host
Synthetic
Species Reactivity
Mouse
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
ATP6AP1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
51,008 Da
3D Structure
ModBase 3D Structure for Q9R1Q9
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ATP6AP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for ATP6AP1 sirna
RNA Interference (RNAi)

Other Notes

Small volumes of ATP6AP1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ATP6AP1. It may not necessarily be applicable to this product.

NCBI GI #
9055172
NCBI GeneID
54411
NCBI Accession #
NP_061264.1
UniProt Primary Accession #
Q9R1Q9
UniProt Related Accession #
Q9R1Q9
GenBank Nucleotide #
NM_018794.4
NCBI Official Full Name
V-type proton ATPase subunit S1
NCBI Official Synonym Full Names
ATPase, H+ transporting, lysosomal accessory protein 1
NCBI Official Symbol
Atp6ap1
NCBI Official Synonym Symbols
16A; CF2; AC45; C7-1; XAP-3; Atp6s1; VATPS1; Atp6ip1; AI316502; AW108110; mFLJ00383
UniProt Protein Name
V-type proton ATPase subunit S1
UniProt Synonym Protein Names
Protein C7-1; V-ATPase Ac45 subunit; V-ATPase S1 accessory protein; Vacuolar proton pump subunit S1
Protein Family
V-type proton ATPase
UniProt Gene Name
Atp6ap1
UniProt Synonym Gene Names
Atp6ip1; Atp6s1; V-ATPase subunit S1
UniProt Entry Name
VAS1_MOUSE
NCBI Protein Information
V-type proton ATPase subunit S1

UniProt Comments for ATP6AP1

ATP6AP1: Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. Belongs to the vacuolar ATPase subunit S1 family.

Protein type: Transporter; Energy Metabolism - oxidative phosphorylation; EC 3.6.3.14; Membrane protein, integral; Hydrolase

Cellular Component: membrane; integral to membrane; vacuole

Molecular Function: nucleotide binding; hydrogen ion transporting ATP synthase activity, rotational mechanism; hydrogen ion transporting ATPase activity, rotational mechanism; ATP binding; Rab GTPase binding

Biological Process: cell death; positive regulation of osteoblast differentiation; proton transport; transport; ATP hydrolysis coupled proton transport; pH reduction; positive regulation of bone resorption; ion transport; positive regulation of exocytosis; establishment of organelle localization
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Research Articles on ATP6AP1

1. The mitochondrial H(+)-ATP synthase, by coupling the Warburg effect to anabolic metabolism, enables de-differentiation during the reprogramming of somatic cells to induced pluripotent stem cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ATP6AP1 sirna

 Reference Product  PubMed Publications
 Atp6ap2 sirna  >2 publications with ATP6AP1 and Atp6ap2
 Atp6v0d2 sirna  >2 publications with ATP6AP1 and Atp6v0d2
 Atp6v1e1 sirna  >1 publications with ATP6AP1 and Atp6v1e1
 Atp6v0d1 sirna  >1 publications with ATP6AP1 and Atp6v0d1

Pathways associated with ATP6AP1 sirna

 Products by Pathway  Pathway Diagram
 Hepatitis B Pathway antibodies  Hepatitis B Pathway Diagram
 Insulin Receptor Recycling Pathway antibodies  Insulin Receptor Recycling Pathway Diagram
 Ion Channel Transport Pathway antibodies  Ion Channel Transport Pathway Diagram
 Iron Uptake And Transport Pathway antibodies  Iron Uptake And Transport Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Oxidative Phosphorylation Pathway antibodies  Oxidative Phosphorylation Pathway Diagram
 Oxidative Phosphorylation Pathway antibodies  Oxidative Phosphorylation Pathway Diagram
 Oxidative Phosphorylation Pathway antibodies  Oxidative Phosphorylation Pathway Diagram

Diseases associated with ATP6AP1 sirna

 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >7 publications with ATP6AP1 and Disease Models, Animal
 Nervous System Diseases Antibodies  >3 publications with ATP6AP1 and Nervous System Diseases
 Inflammation Antibodies  >2 publications with ATP6AP1 and Inflammation
 Neoplasm Metastasis Antibodies  >2 publications with ATP6AP1 and Neoplasm Metastasis
 Neurobehavioral Manifestations Antibodies  >1 publications with ATP6AP1 and Neurobehavioral Manifestations
 Congenital Abnormalities Antibodies  >1 publications with ATP6AP1 and Congenital Abnormalities
 Heart Diseases Antibodies  >1 publications with ATP6AP1 and Heart Diseases
 Eye Abnormalities Antibodies  >1 publications with ATP6AP1 and Eye Abnormalities
 Fibrosis Antibodies  >1 publications with ATP6AP1 and Fibrosis
 Breast Neoplasms Antibodies  >1 publications with ATP6AP1 and Breast Neoplasms

Organs/Tissues associated with ATP6AP1 sirna

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >26 publications with ATP6AP1 and Bone
 Bone Marrow Antibodies  >6 publications with ATP6AP1 and Bone Marrow
 Brain Antibodies  >5 publications with ATP6AP1 and Brain
 Embryonic Tissue Antibodies  >4 publications with ATP6AP1 and Embryonic Tissue
 Eye Antibodies  >3 publications with ATP6AP1 and Eye
 Liver Antibodies  >2 publications with ATP6AP1 and Liver
 Connective Tissue Antibodies  >2 publications with ATP6AP1 and Connective Tissue
 Kidney Antibodies  >2 publications with ATP6AP1 and Kidney
 Pancreas Antibodies  >2 publications with ATP6AP1 and Pancreas
 Lung Antibodies  >1 publications with ATP6AP1 and Lung
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