BARHL2 siRNA – Barh-Like 2 Homeobox Protein (Mouse)

Name: BARHL2 siRNA; Barh-Like 2 Homeobox Protein; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS8221931
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8221931

MBS8221931 is an siRNA targeting BARHL2. Also known as BARHL2 siRNA (Mouse). Reactive in Mouse. Host: Synthetic. siRNA to inhibit BARHL2 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

BARHL2, siRNA

Full Product Name

BARHL2 siRNA (Mouse)

Product Gene Name

BARHL2 sirna

Product Synonym Names

BARH1; MBH1; BarH-like 2 homeobox protein; Bar-class homeodomain protein MBH1; Homeobox protein B-H1

Host

Synthetic

Species Reactivity

Mouse

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

BARHL2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

41,459 Da

3D Structure

ModBase 3D Structure for Q8VIB5

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse BARHL2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for BARHL2 sirna

RNA Interference (RNAi)

Other Notes

Small volumes of BARHL2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for BARHL2. It may not necessarily be applicable to this product.

NCBI GI #

56699436

NCBI GeneID

104382

NCBI Accession #

NP_001005477.1

UniProt Primary Accession #

Q8VIB5

UniProt Secondary Accession #

Q66L43

UniProt Related Accession #

Q8VIB5

GenBank Nucleotide #

NM_001005477.1

NCBI Official Full Name

barH-like 2 homeobox protein

NCBI Official Synonym Full Names

BarH-like 2 (Drosophila)

NCBI Official Symbol

Barhl2

NCBI Official Synonym Symbols

MBH1; E130309B19Rik

UniProt Protein Name

BarH-like 2 homeobox protein

UniProt Synonym Protein Names

Bar-class homeodomain protein MBH1; Homeobox protein B-H1

Protein Family

BarH-like 2 homeobox protein

UniProt Gene Name

Barhl2

UniProt Synonym Gene Names

Barh1; Mbh1

UniProt Entry Name

BARH2_MOUSE

NCBI Protein Information

barH-like 2 homeobox protein

UniProt Comments for BARHL2

BARHL2: Potential regulator of neural basic helix-loop-helix genes. Belongs to the BAR homeobox family.

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: DNA binding; sequence-specific DNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; nervous system development; cell fate commitment; transcription, DNA-dependent; positive regulation of translation; multicellular organismal development; neuron migration; regulation of axon extension; cell fate determination; neuron differentiation; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter

Research Articles on BARHL2

1. results elucidate BARHL2 as a critical postmitotic regulator of dI1 subtype diversification, as well as its intermediate position in the dI1 genetic hierarchy

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with BARHL2 sirna

Reference Product	PubMed Publications
Atoh7 sirna	>4 publications with BARHL2 and Atoh7
Lhx2 sirna	>3 publications with BARHL2 and Lhx2
Atoh1 sirna	>2 publications with BARHL2 and Atoh1
Bhlhe22 sirna	>1 publications with BARHL2 and Bhlhe22
Neurod4 sirna	>1 publications with BARHL2 and Neurod4
Ptf1a sirna	>1 publications with BARHL2 and Ptf1a
Pou4f1 sirna	>1 publications with BARHL2 and Pou4f1

Diseases associated with BARHL2 sirna

Disease Name	Pubmed Publications
Diabetes Mellitus Antibodies	>1 publications with BARHL2 and Diabetes Mellitus
Glucose Intolerance Antibodies	>1 publications with BARHL2 and Glucose Intolerance

Organs/Tissues associated with BARHL2 sirna

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>8 publications with BARHL2 and Brain
Eye Antibodies	>7 publications with BARHL2 and Eye