BLM siRNA – Protein Homolog Isoform 2 (Mouse)

Name: BLM siRNA; Protein Homolog Isoform 2; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS8200007
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8200007

MBS8200007 is an siRNA targeting BLM. Also known as BLM siRNA (Mouse). Reactive in Mouse. Host: Synthetic. siRNA to inhibit BLM expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

BLM, siRNA

Full Product Name

BLM siRNA (Mouse)

Product Gene Name

BLM sirna

Product Synonym Names

Bloom syndrome protein homolog; mBLM; RecQ helicase homolog

Host

Synthetic

Species Reactivity

Mouse

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

BLM siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

158,366 Da

3D Structure

ModBase 3D Structure for O88700

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse BLM gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for BLM sirna

RNA Interference (RNAi)

Other Notes

Small volumes of BLM sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for BLM. It may not necessarily be applicable to this product.

NCBI GI #

110225360

NCBI GeneID

12144

NCBI Accession #

NP_001035992.1

UniProt Primary Accession #

O88700

UniProt Secondary Accession #

O88198

UniProt Related Accession #

O88700

GenBank Nucleotide #

NM_001042527.2

NCBI Official Full Name

Bloom syndrome protein homolog isoform 2

NCBI Official Synonym Full Names

Bloom syndrome, RecQ helicase-like

NCBI Official Symbol

Blm

UniProt Protein Name

Bloom syndrome protein homolog

UniProt Synonym Protein Names

RecQ helicase homolog

Protein Family

Bloom syndrome protein

UniProt Gene Name

Blm

UniProt Synonym Gene Names

mBLM

UniProt Entry Name

BLM_MOUSE

NCBI Protein Information

Bloom syndrome protein homolog

UniProt Comments for BLM

BLM: a magnesium-dependent ATP-dependent DNA-helicase that unwinds single- and double-stranded DNA in a 3'-5' direction. A member of the RecQ helicase family that is required for genome stability. Participates in DNA replication, recombination and repair. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex which is a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with ubiquitinated FANCD2.

Protein type: Helicase; DNA repair, damage; DNA replication; EC 3.6.4.12

Cellular Component: male germ cell nucleus; lateral element; nuclear chromosome; chromosome, telomeric region; PML body; nuclear matrix; pronucleus; cytoplasm; nucleolus; replication fork; intracellular; nucleus

Molecular Function: G-quadruplex DNA binding; hydrolase activity; p53 binding; ATPase activity; nucleotide binding; ATP-dependent helicase activity; bubble DNA binding; helicase activity; four-way junction helicase activity; ATP-dependent DNA helicase activity; protein binding; nucleic acid binding; DNA binding; ATP-dependent 3'-5' DNA helicase activity; catalytic activity; ATP binding; single-stranded DNA binding; hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides

Biological Process: negative regulation of DNA recombination; regulation of binding; positive regulation of transcription, DNA-dependent; negative regulation of mitotic recombination; DNA double-strand break processing; alpha-beta T cell differentiation; negative regulation of cell division; DNA repair; chromosome organization and biogenesis; DNA duplex unwinding; protein oligomerization; DNA recombination; replication fork processing; cellular metabolic process; mitotic cell cycle G2/M transition DNA damage checkpoint; DNA strand renaturation; positive regulation of alpha-beta T cell proliferation; regulation of cyclin-dependent protein kinase activity; DNA replication; response to DNA damage stimulus; telomere maintenance; response to X-ray

Research Articles on BLM

1. Data suggest that BLM suppresses homologous recombination, while full-length BRCA1 promotes this process.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with BLM sirna

Reference Product	PubMed Publications
Rad51 sirna	>92 publications with BLM and Rad51
Atm sirna	>64 publications with BLM and Atm
Mlh1 sirna	>18 publications with BLM and Mlh1
Rpa1 sirna	>13 publications with BLM and Rpa1
Fancm sirna	>12 publications with BLM and Fancm
Dmc1 sirna	>5 publications with BLM and Dmc1
Mlh3 sirna	>4 publications with BLM and Mlh3
Top3a sirna	>3 publications with BLM and Top3a

Pathways associated with BLM sirna

Products by Pathway	Pathway Diagram
BRCA1-associated Genome Surveillance Complex (BASC) Pathway antibodies	BRCA1-associated Genome Surveillance Complex (BASC) Pathway Diagram
BRCA1-associated Genome Surveillance Complex (BASC) Pathway antibodies	BRCA1-associated Genome Surveillance Complex (BASC) Pathway Diagram
Bloom's Syndrome Complex Pathway antibodies	Bloom's Syndrome Complex Pathway Diagram
Bloom's Syndrome Complex Pathway antibodies	Bloom's Syndrome Complex Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Fanconi Anemia Pathway antibodies	Fanconi Anemia Pathway Diagram
Fanconi Anemia Pathway antibodies	Fanconi Anemia Pathway Diagram
Homologous Recombination Pathway antibodies	Homologous Recombination Pathway Diagram
Homologous Recombination Pathway antibodies	Homologous Recombination Pathway Diagram
Meiosis Pathway antibodies	Meiosis Pathway Diagram

Diseases associated with BLM sirna

Disease Name	Pubmed Publications
Bloom Syndrome Antibodies	>500 publications with BLM and Bloom Syndrome
Fibrosis Antibodies	>424 publications with BLM and Fibrosis
Disease Models, Animal Antibodies	>223 publications with BLM and Disease Models, Animal
Neoplasms, Experimental Antibodies	>142 publications with BLM and Neoplasms, Experimental
Chromosome Aberrations Antibodies	>138 publications with BLM and Chromosome Aberrations
Inflammation Antibodies	>135 publications with BLM and Inflammation
Skin Diseases Antibodies	>110 publications with BLM and Skin Diseases
Necrosis Antibodies	>88 publications with BLM and Necrosis
Neoplasm Metastasis Antibodies	>67 publications with BLM and Neoplasm Metastasis
Anemia Antibodies	>65 publications with BLM and Anemia

Organs/Tissues associated with BLM sirna

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>496 publications with BLM and Lung
Intestine Antibodies	>93 publications with BLM and Intestine
Bone Antibodies	>76 publications with BLM and Bone
Brain Antibodies	>59 publications with BLM and Brain
Bone Marrow Antibodies	>46 publications with BLM and Bone Marrow
Spleen Antibodies	>40 publications with BLM and Spleen
Ovary Antibodies	>35 publications with BLM and Ovary
Heart Antibodies	>27 publications with BLM and Heart
Thymus Antibodies	>25 publications with BLM and Thymus
Embryonic Tissue Antibodies	>23 publications with BLM and Embryonic Tissue