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CDK2 siRNA – Cyclin-Dependent Kinase 2 Isoform 2 (Mouse)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8215067

MBS8215067 is an siRNA targeting CDK2. Also known as CDK2 siRNA (Mouse). Reactive in Mouse. Host: Synthetic. siRNA to inhibit CDK2 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CDK2, siRNA
Full Product Name
CDK2 siRNA (Mouse)
Product Gene Name
CDK2 sirna
Product Synonym Names
CDKN2; Cyclin-dependent kinase 2; Cell division protein kinase 2
Host
Synthetic
Species Reactivity
Mouse
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
CDK2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
33,887 Da
3D Structure
ModBase 3D Structure for P97377
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CDK2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for CDK2 sirna
RNA Interference (RNAi)

Other Notes

Small volumes of CDK2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CDK2. It may not necessarily be applicable to this product.

NCBI GI #
7949020
NCBI GeneID
12566
NCBI Accession #
NP_058036.1
UniProt Primary Accession #
P97377
UniProt Secondary Accession #
O55105
UniProt Related Accession #
P97377
GenBank Nucleotide #
NM_016756.4
NCBI Official Full Name
cyclin-dependent kinase 2 isoform 2
NCBI Official Synonym Full Names
cyclin-dependent kinase 2
NCBI Official Symbol
Cdk2
NCBI Official Synonym Symbols
A630093N05Rik
UniProt Protein Name
Cyclin-dependent kinase 2
UniProt Synonym Protein Names
Cell division protein kinase 2
Protein Family
Cyclin-dependent kinase
UniProt Gene Name
Cdk2
UniProt Synonym Gene Names
Cdkn2
UniProt Entry Name
CDK2_MOUSE
NCBI Protein Information
cyclin-dependent kinase 2

UniProt Comments for CDK2

CDK2: a protein kinase of the CDK family. An important component of the cell cycle machinery. Activity of CDK2 is maximal during S phase and G2. Cdk2/cyclin E kinase activity is important for the G1 to S transition and phosphorylates the Rb protein. In S-phase, active cdk2/cyclin A complexes predominate and phosphorylate E2F, and the active cdk2 complex persists in the nucleus through G2. Part of the Rb pathway disregulated in most tumors. Target of several candidate cancer drugs. However, inhibition does not always prevent cancer cell growth, possibly due to CDK redundancy. Inhibitors: BMS-265246, BMS-265246-01, R-roscovitine (CYC200, CYC202), SU9516, R547, L868276

Protein type: EC 2.7.11.22; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; Cell cycle regulation; CMGC group; CDK family; CDK/CDK1 subfamily; CDK1 subfamily

Cellular Component: Cajal body; chromosome, telomeric region; intracellular membrane-bound organelle; X chromosome; condensed chromosome; cytosol; Y chromosome; transcription factor complex; cytoskeleton; cytoplasm; cyclin-dependent protein kinase holoenzyme complex; nucleus; endosome

Molecular Function: transferase activity; protein serine/threonine kinase activity; protein binding; cyclin binding; cyclin-dependent protein kinase activity; metal ion binding; transferase activity, transferring phosphorus-containing groups; protein complex binding; nucleotide binding; histone kinase activity; kinase activity; ATP binding; protein kinase activity

Biological Process: mitosis; response to cAMP; regulation of cell cycle; histone phosphorylation; positive regulation of transcription, DNA-dependent; cell cycle; DNA repair; protein amino acid phosphorylation; peptidyl-serine phosphorylation; meiotic cell cycle; cellular response to insulin stimulus; cell division; positive regulation of cell proliferation; response to electrical stimulus; phosphorylation; response to DNA damage stimulus; potassium ion transport; G1/S transition of mitotic cell cycle; positive regulation of DNA replication initiation
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Research Articles on CDK2

1. ablation of the kinase CDK2 alters the nuclear envelope in mouse spermatocytes, and that the proteins SUN1, KASH5 (also known as CCDC155) and lamin C2 show an abnormal cap-like distribution facing the centrosome.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CDK2 sirna

 Reference Product  PubMed Publications
 Cdkn1a sirna  >1244 publications with CDK2 and Cdkn1a
 Cdkn1b sirna  >1145 publications with CDK2 and Cdkn1b
 Cdc25a sirna  >189 publications with CDK2 and Cdc25a
 Ccna2 sirna  >94 publications with CDK2 and Ccna2
 Ccne1 sirna  >87 publications with CDK2 and Ccne1
 Cdc25c sirna  >79 publications with CDK2 and Cdc25c
 Cdc6 sirna  >72 publications with CDK2 and Cdc6
 Rbl1 sirna  >54 publications with CDK2 and Rbl1
 Rb1 sirna  >25 publications with CDK2 and Rb1
 Ccna1 sirna  >20 publications with CDK2 and Ccna1

Pathways associated with CDK2 sirna

 Products by Pathway  Pathway Diagram
 APC/C-mediated Degradation Of Cell Cycle Proteins Pathway antibodies  APC/C-mediated Degradation Of Cell Cycle Proteins Pathway Diagram
 Activation Of ATR In Response To Replication Stress Pathway antibodies  Activation Of ATR In Response To Replication Stress Pathway Diagram
 Activation Of The Pre-replicative Complex Pathway antibodies  Activation Of The Pre-replicative Complex Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 CDK-mediated Phosphorylation And Removal Of Cdc6 Pathway antibodies  CDK-mediated Phosphorylation And Removal Of Cdc6 Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Checkpoints Pathway antibodies  Cell Cycle Checkpoints Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram

Diseases associated with CDK2 sirna

 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >377 publications with CDK2 and Breast Neoplasms
 Adenocarcinoma Antibodies  >296 publications with CDK2 and Adenocarcinoma
 Neoplasms, Experimental Antibodies  >198 publications with CDK2 and Neoplasms, Experimental
 Liver Diseases Antibodies  >174 publications with CDK2 and Liver Diseases
 Liver Neoplasms Antibodies  >164 publications with CDK2 and Liver Neoplasms
 Carcinoma, Hepatocellular Antibodies  >151 publications with CDK2 and Carcinoma, Hepatocellular
 Prostatic Neoplasms Antibodies  >149 publications with CDK2 and Prostatic Neoplasms
 Lung Neoplasms Antibodies  >145 publications with CDK2 and Lung Neoplasms
 Nervous System Diseases Antibodies  >132 publications with CDK2 and Nervous System Diseases
 Colonic Neoplasms Antibodies  >100 publications with CDK2 and Colonic Neoplasms

Organs/Tissues associated with CDK2 sirna

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >416 publications with CDK2 and Blood
 Liver Antibodies  >309 publications with CDK2 and Liver
 Lung Antibodies  >263 publications with CDK2 and Lung
 Brain Antibodies  >170 publications with CDK2 and Brain
 Kidney Antibodies  >144 publications with CDK2 and Kidney
 Bone Antibodies  >122 publications with CDK2 and Bone
 Skin Antibodies  >115 publications with CDK2 and Skin
 Embryonic Tissue Antibodies  >98 publications with CDK2 and Embryonic Tissue
 Heart Antibodies  >86 publications with CDK2 and Heart
 Eye Antibodies  >60 publications with CDK2 and Eye
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