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CLIC5 siRNA – Chloride Intracellular Channel Protein 5 (Mouse)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8218247

MBS8218247 is an siRNA targeting CLIC5. Also known as CLIC5 siRNA (Mouse). Reactive in Mouse. Host: Synthetic. siRNA to inhibit CLIC5 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CLIC5, siRNA
Full Product Name
CLIC5 siRNA (Mouse)
Product Gene Name
CLIC5 sirna
Product Synonym Names
Chloride intracellular channel protein 5
Host
Synthetic
Species Reactivity
Mouse
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
CLIC5 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
28,287 Da
3D Structure
ModBase 3D Structure for Q8BXK9
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CLIC5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for CLIC5 sirna
RNA Interference (RNAi)

Other Notes

Small volumes of CLIC5 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CLIC5. It may not necessarily be applicable to this product.

NCBI GI #
27369886
NCBI GeneID
224796
NCBI Accession #
NP_766209.1
UniProt Primary Accession #
Q8BXK9
UniProt Secondary Accession #
Q3U0H8
UniProt Related Accession #
Q8BXK9
GenBank Nucleotide #
NM_172621.2
NCBI Official Full Name
chloride intracellular channel protein 5
NCBI Official Synonym Full Names
chloride intracellular channel 5
NCBI Official Symbol
Clic5
NCBI Official Synonym Symbols
jbg; Gm322; B330005L24; 5730531E12Rik
UniProt Protein Name
Chloride intracellular channel protein 5
Protein Family
Chloride intracellular channel protein
UniProt Gene Name
Clic5
UniProt Entry Name
CLIC5_MOUSE
NCBI Protein Information
chloride intracellular channel protein 5

UniProt Comments for CLIC5

CLIC5: Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. May play a role in the regulation of transepithelial ion absorption and secretion. Required for normal formation of stereocilia in the inner ear and normal development of the organ of Corti. Is required for the development and/or maintenance of the proper glomerular endothelial cell and podocyte architecture. Belongs to the chloride channel CLIC family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis

Cellular Component: Golgi apparatus; stereocilium; cytoskeleton; stereocilium bundle; membrane; cytoplasm; integral to membrane; actin cytoskeleton

Molecular Function: chloride channel activity; voltage-gated ion channel activity

Biological Process: response to dietary excess; protein localization; diet induced thermogenesis; sensory perception of sound; transport; ion transport; chloride transport; auditory receptor cell stereocilium organization and biogenesis; neuromuscular process controlling balance
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Research Articles on CLIC5

1. Biochemical assays showed interaction of CLIC5 with ERM proteins, TPRN, and possibly myosin VI (MYO6). In addition, CLIC5 and RDX failed to localize normally in fused stereocilia of MYO6 mutant mice

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CLIC5 sirna

 Reference Product  PubMed Publications
 Clic3 sirna  >3 publications with CLIC5 and Clic3
 Akap9 sirna  >1 publications with CLIC5 and Akap9

Pathways associated with CLIC5 sirna

 Products by Pathway  Pathway Diagram
 PodNet: Protein-protein Interactions In The Podocyte Pathway antibodies  PodNet: Protein-protein Interactions In The Podocyte Pathway Diagram
 XPodNet - Protein-protein Interactions In The Podocyte Expanded By STRING Pathway antibodies  XPodNet - Protein-protein Interactions In The Podocyte Expanded By STRING Pathway Diagram

Diseases associated with CLIC5 sirna

 Disease Name  Pubmed Publications
 Proteinuria Antibodies  >2 publications with CLIC5 and Proteinuria
 Neoplasm Metastasis Antibodies  >2 publications with CLIC5 and Neoplasm Metastasis
 Kidney Diseases Antibodies  >1 publications with CLIC5 and Kidney Diseases
 Carcinoma, Hepatocellular Antibodies  >1 publications with CLIC5 and Carcinoma, Hepatocellular
 Fibrosis Antibodies  >1 publications with CLIC5 and Fibrosis

Organs/Tissues associated with CLIC5 sirna

 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >6 publications with CLIC5 and Kidney
 Brain Antibodies  >4 publications with CLIC5 and Brain
 Muscle Antibodies  >3 publications with CLIC5 and Muscle
 Heart Antibodies  >3 publications with CLIC5 and Heart
 Embryonic Tissue Antibodies  >2 publications with CLIC5 and Embryonic Tissue
 Lung Antibodies  >1 publications with CLIC5 and Lung
 Extraembryonic Tissue Antibodies  >1 publications with CLIC5 and Extraembryonic Tissue
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