CML2 siRNA – Probable N-Acetyltransferase CML2 (Mouse)

Name: CML2 siRNA; Probable N-Acetyltransferase CML2; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS8230338
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8230338

MBS8230338 is an siRNA targeting CML2. Also known as CML2 siRNA (Mouse). Reactive in Mouse. Host: Synthetic. siRNA to inhibit CML2 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CML2, siRNA

Full Product Name

CML2 siRNA (Mouse)

Product Gene Name

CML2 sirna

Product Synonym Names

Probable N-acetyltransferase CML2; Camello-like protein 2

Host

Synthetic

Species Reactivity

Mouse

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

CML2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

26,424 Da

3D Structure

ModBase 3D Structure for Q8CHQ9

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CML2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for CML2 sirna

RNA Interference (RNAi)

Other Notes

Small volumes of CML2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CML2. It may not necessarily be applicable to this product.

NCBI GI #

31542409

NCBI GeneID

93673

NCBI Accession #

NP_444326.2

UniProt Primary Accession #

Q8CHQ9

UniProt Secondary Accession #

Q8C7E5; Q9JIY9

UniProt Related Accession #

Q8CHQ9

GenBank Nucleotide #

NM_053096.3

NCBI Official Full Name

probable N-acetyltransferase CML2

NCBI Official Synonym Full Names

camello-like 2

NCBI Official Symbol

Cml2

UniProt Protein Name

Probable N-acetyltransferase CML2

UniProt Synonym Protein Names

Camello-like protein 2

Protein Family

Calmodulin-like protein

UniProt Gene Name

Cml2

UniProt Entry Name

CMLO2_MOUSE

NCBI Protein Information

probable N-acetyltransferase CML2

UniProt Comments for CML2

CML2: May play a role in regulation of gastrulation. Belongs to the camello family.

Protein type: Acetyltransferase; Membrane protein, integral; EC 2.3.1.-

Cellular Component: Golgi apparatus; extracellular space; membrane; endoplasmic reticulum; integral to membrane

Molecular Function: keto acid formate lyase activity; glucosaminyl-phosphotidylinositol O-acyltransferase activity; S-malonyltransferase activity; 3-hydroxybutyryl-CoA thiolase activity; S-acetyltransferase activity; O-octanoyltransferase activity; O-sinapoyltransferase activity; acyl-CoA N-acyltransferase activity; peptidyl-lysine N6-myristoyltransferase activity; palmitoleoyl [acyl-carrier-protein]-dependent acyltransferase activity; peptidyl-lysine N6-palmitoyltransferase activity; azetidine-2-carboxylic acid acetyltransferase activity; dihydrolipoamide branched chain acyltransferase activity; succinyltransferase activity; Ras palmitoyltransferase activity; palmitoyltransferase activity; C-acyltransferase activity; C-palmitoyltransferase activity; dihydrolipoamide S-acyltransferase activity; N-acetyltransferase activity; protein-cysteine S-acyltransferase activity; serine O-acyltransferase activity; malonyltransferase activity; octanoyltransferase activity; O-palmitoyltransferase activity; O-succinyltransferase activity; sterol O-acyltransferase activity; carnitine O-acyltransferase activity; 3-ketopimelyl-CoA thiolase activity; S-succinyltransferase activity; N-acyltransferase activity; UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase activity; O-acyltransferase activity; N-palmitoyltransferase activity; acylglycerol O-acyltransferase activity; O-acetyltransferase activity; transferase activity; benzoyl acetate-CoA thiolase activity; sinapoyltransferase activity; protein-cysteine S-myristoyltransferase activity; S-acyltransferase activity; myristoyltransferase activity; acetyltransferase activity; N-succinyltransferase activity; transferase activity, transferring acyl groups; L-2-aminoadipate N-acetyltransferase activity

Biological Process: multicellular organismal development; gastrulation; gastrulation with mouth forming second; negative regulation of cell adhesion

Research Articles on CML2

1. Biochemical inhibition of the acetyltransferases ATase1 and ATase2 reduces beta-secretase (BACE1) levels and Abeta generation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.