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COPS6 siRNA – COP9 Signalosome Complex Subunit 6 (Mouse)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8205976

MBS8205976 is an siRNA targeting COPS6. Also known as COPS6 siRNA (Mouse). Reactive in Mouse. Host: Synthetic. siRNA to inhibit COPS6 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
COPS6, siRNA
Full Product Name
COPS6 siRNA (Mouse)
Product Gene Name
COPS6 sirna
Product Synonym Names
CSN6; COP9 signalosome complex subunit 6; SGN6; Signalosome subunit 6; JAB1-containing signalosome subunit 6
Host
Synthetic
Species Reactivity
Mouse
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
COPS6 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
35,880 Da
3D Structure
ModBase 3D Structure for O88545
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse COPS6 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for COPS6 sirna
RNA Interference (RNAi)

Other Notes

Small volumes of COPS6 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for COPS6. It may not necessarily be applicable to this product.

NCBI GI #
33563284
NCBI GeneID
26893
NCBI Accession #
NP_036132.1
UniProt Primary Accession #
O88545
UniProt Related Accession #
O88545
GenBank Nucleotide #
NM_012002.3
NCBI Official Full Name
COP9 signalosome complex subunit 6
NCBI Official Synonym Full Names
COP9 (constitutive photomorphogenic) homolog, subunit 6 (Arabidopsis thaliana)
NCBI Official Symbol
Cops6
NCBI Official Synonym Symbols
Sgn3; VIP/MOV34
UniProt Protein Name
COP9 signalosome complex subunit 6
UniProt Synonym Protein Names
JAB1-containing signalosome subunit 6
UniProt Gene Name
Cops6
UniProt Synonym Gene Names
Csn6; SGN6; Signalosome subunit 6
UniProt Entry Name
CSN6_MOUSE
NCBI Protein Information
COP9 signalosome complex subunit 6

UniProt Comments for COPS6

COPS6: Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Has some glucocorticoid receptor- responsive activity. Stabilizes RFWD2/COP1 through reducing RFWD2 auto-ubiquitination and decelerating RFWD2 turnover rate, hence regulates the ubiquitination of RFWD2 targets. Belongs to the peptidase M67A family. CSN6 subfamily.

Protein type: Cell cycle regulation

Cellular Component: signalosome; nucleoplasm; cytoplasm; nucleus

Molecular Function: protein binding

Biological Process: cullin deneddylation
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Research Articles on COPS6

1. loss of CSN6 enhances p53-mediated tumor suppression in vivo and CSN6 plays an important role in regulating DNA damage-associated apoptosis and tumorigenesis through control of the MDM2-p53 signaling pathway

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with COPS6 sirna

 Reference Product  PubMed Publications
 Cops5 sirna  >3 publications with COPS6 and Cops5
 Cul4a sirna  >1 publications with COPS6 and Cul4a
 Cops2 sirna  >1 publications with COPS6 and Cops2
 Cops4 sirna  >1 publications with COPS6 and Cops4

Diseases associated with COPS6 sirna

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >1 publications with COPS6 and Necrosis

Organs/Tissues associated with COPS6 sirna

 Organ/Tissue Name  Pubmed Publications
 Stomach Antibodies  >1 publications with COPS6 and Stomach
 Lymph Node Antibodies  >1 publications with COPS6 and Lymph Node
 Brain Antibodies  >1 publications with COPS6 and Brain
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