CRY1 siRNA – Cryptochrome-1 (Mouse)

This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by Clock/Arntl heterodimers but then represses this upregulation in a feedback loop using Per/Cry heterodimers to interact with Clock/Arntl. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. Loss of this gene results in a shortened circadian cycle in complete darkness. [provided by RefSeq, Feb 2014]

CRY1: Blue light-dependent regulator of the circadian feedback loop. Inhibits CLOCK|NPAS2-ARNTL E box-mediated transcription. Acts, in conjunction with CRY2, in maintaining period length and circadian rhythmicity. Has no photolyase activity. Capable of translocating circadian clock core proteins such as PER proteins to the nucleus. May inhibit CLOCK|NPAS2-ARNTL transcriptional activity through stabilizing the unphosphorylated form of ARNTL. Component of the circadian core oscillator, which includes the CRY proteins, CLOCK or NPAS2, ARNTL or ARNTL2, CSNK1D and/or CSNK1E, TIMELESS, and the PER proteins. Interacts directly with TIMELESS and the PER proteins. Interacts directly with PER1 and PER2 C-terminal domains. Interaction with PER2 inhibits its ubiquitination and vice versa. Binds MAPK. Interacts with FBXL21. Interacts with FBXL3. Expression is regulated by light and circadian rhythms. Peak expression in the suprachiasma nucleus (SCN) and eye at the day/night transition (CT12). Levels decrease with ARNTL-CLOCK inhibition as part of the autoregulatory feedback loop. Belongs to the DNA photolyase class-1 family.

Protein type: Lyase; Transcription, coactivator/corepressor; DNA-binding

Cellular Component: mitochondrion; cytoplasm; nucleus

Molecular Function: DNA photolyase activity; ubiquitin binding; protein binding; histone deacetylase binding; double-stranded DNA binding; nucleotide binding; photoreceptor activity; transcription factor binding; kinase binding; protein kinase binding; nuclear hormone receptor binding; phosphatase binding

Biological Process: circadian rhythm; response to glucagon stimulus; transcription, DNA-dependent; DNA damage induced protein phosphorylation; negative regulation of G-protein coupled receptor protein signaling pathway; response to stimulus; rhythmic process; negative regulation of transcription from RNA polymerase II promoter; DNA repair; glucose homeostasis; regulation of circadian rhythm; protein-chromophore linkage; gluconeogenesis; entrainment of circadian clock by photoperiod; response to insulin stimulus; sequestering of lipid; regulation of transcription, DNA-dependent; negative regulation of protein ubiquitination; negative regulation of circadian rhythm; circadian regulation of gene expression; negative regulation of transcription, DNA-dependent