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CTSG siRNA – Cathepsin G Preproprotein (Mouse)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8228300

MBS8228300 is an siRNA targeting CTSG. Also known as CTSG siRNA (Mouse). Reactive in Mouse. Host: Synthetic. siRNA to inhibit CTSG expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CTSG, siRNA
Full Product Name
CTSG siRNA (Mouse)
Product Gene Name
CTSG sirna
Product Synonym Names
Cathepsin G; Vimentin-specific protease; VSP
Host
Synthetic
Species Reactivity
Mouse
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
CTSG siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
29,096 Da
3D Structure
ModBase 3D Structure for P28293
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CTSG gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for CTSG sirna
RNA Interference (RNAi)

Other Notes

Small volumes of CTSG sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CTSG. It may not necessarily be applicable to this product.

NCBI GI #
6681083
NCBI GeneID
13035
NCBI Accession #
NP_031826.1
UniProt Primary Accession #
P28293
UniProt Related Accession #
P28293
GenBank Nucleotide #
NM_007800.2
NCBI Official Full Name
cathepsin G preproprotein
NCBI Official Synonym Full Names
cathepsin G
NCBI Official Symbol
Ctsg
NCBI Official Synonym Symbols
VSP; CatG
UniProt Protein Name
Cathepsin G
UniProt Synonym Protein Names
Vimentin-specific protease; VSP
Protein Family
Cathepsin
UniProt Gene Name
Ctsg
UniProt Synonym Gene Names
VSP
UniProt Entry Name
CATG_MOUSE
NCBI Protein Information
cathepsin G

NCBI Summary for CTSG

This gene encodes a member of the peptidase S1 (chymotrypsin) family of serine endopeptidases. The encoded preproprotein is proteolytically processed to generate a mature protein product. This product appears to play a role in host defense, including inflammation and antigen processing. Homozygous knockout mice for this gene exhibit enhanced susceptibility to bacterial and fungal infection. [provided by RefSeq, Aug 2015]
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UniProt Comments for CTSG

CTSG: Serine protease with trypsin- and chymotrypsin-like specificity. Cleaves complement C3. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, antibacterial activity is inhibited by LPS from P.aeruginosa, Z-Gly-Leu-Phe- CH2Cl and phenylmethylsulfonyl fluoride. Belongs to the peptidase S1 family.

Protein type: EC 3.4.21.20; Motility/polarity/chemotaxis; Protease

Cellular Component: extracellular matrix; extracellular space; membrane; intracellular membrane-bound organelle; cytoplasm; plasma membrane; intermediate filament; nucleus; secretory granule

Molecular Function: heparin binding; peptidase activity; hydrolase activity; serine-type peptidase activity; serine-type endopeptidase activity; catalytic activity

Biological Process: defense response to Gram-positive bacterium; positive regulation of immune response; response to lipopolysaccharide; protein processing; proteolysis; defense response to fungus
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Research Articles on CTSG

1. Cathepsin G activity may impair efferocytosis, which could lead to an accumulation of lesion-associated apoptotic cells and the accelerated progression of early atherosclerotic lesions to more complex lesions in Apoe(-/-) mice.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with CTSG sirna

 Products by Pathway  Pathway Diagram
 Activation Of Matrix Metalloproteinases Pathway antibodies  Activation Of Matrix Metalloproteinases Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Degradation Of The Extracellular Matrix Pathway antibodies  Degradation Of The Extracellular Matrix Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Metabolism Of Angiotensinogen To Angiotensins Pathway antibodies  Metabolism Of Angiotensinogen To Angiotensins Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram

Diseases associated with CTSG sirna

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >146 publications with CTSG and Inflammation
 Lung Diseases Antibodies  >61 publications with CTSG and Lung Diseases
 Cardiovascular Diseases Antibodies  >45 publications with CTSG and Cardiovascular Diseases
 Leukemia Antibodies  >36 publications with CTSG and Leukemia
 Necrosis Antibodies  >33 publications with CTSG and Necrosis
 Fibrosis Antibodies  >28 publications with CTSG and Fibrosis
 Kidney Diseases Antibodies  >10 publications with CTSG and Kidney Diseases
 Carcinoma Antibodies  >10 publications with CTSG and Carcinoma
 Hemorrhage Antibodies  >10 publications with CTSG and Hemorrhage
 Edema Antibodies  >6 publications with CTSG and Edema

Organs/Tissues associated with CTSG sirna

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >621 publications with CTSG and Blood
 Bone Antibodies  >41 publications with CTSG and Bone
 Bone Marrow Antibodies  >30 publications with CTSG and Bone Marrow
 Spleen Antibodies  >7 publications with CTSG and Spleen
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