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FNDC3B siRNA – Fibronectin Type III Domain-Containing Protein 3b (Mouse)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8227175

MBS8227175 is an siRNA targeting FNDC3B. Also known as FNDC3B siRNA (Mouse). Reactive in Mouse. Host: Synthetic. siRNA to inhibit FNDC3B expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
FNDC3B, siRNA
Full Product Name
FNDC3B siRNA (Mouse)
Product Gene Name
Product Synonym Names
FAD104; KIAA4164; NS5ABP37; Fibronectin type III domain-containing protein 3B; Factor for adipocyte differentiation 104; HCV NS5A-binding protein 37
Host
Synthetic
Species Reactivity
Mouse
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
FNDC3B siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
132,764 Da
3D Structure
ModBase 3D Structure for Q6NWW9
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse FNDC3B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for FNDC3B sirna
RNA Interference (RNAi)

Other Notes

Small volumes of FNDC3B sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for FNDC3B. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
fibronectin type III domain-containing protein 3B
NCBI Official Synonym Full Names
fibronectin type III domain containing 3B
NCBI Official Symbol
Fndc3b
NCBI Official Synonym Symbols
Fad104; AW550168; mKIAA4164; 1600019O04Rik
UniProt Protein Name
Fibronectin type III domain-containing protein 3B
UniProt Synonym Protein Names
Factor for adipocyte differentiation 104; HCV NS5A-binding protein 37
UniProt Gene Name
Fndc3b
UniProt Synonym Gene Names
Fad104; Kiaa4164; Ns5abp37
UniProt Entry Name
FND3B_MOUSE
NCBI Protein Information
fibronectin type III domain-containing protein 3B

UniProt Comments for FNDC3B

FNDC3B: May be a positive regulator of adipogenesis. Belongs to the FNDC3 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: membrane; endoplasmic reticulum; integral to membrane

Biological Process: positive regulation of fibroblast proliferation; cell migration; positive regulation of fat cell differentiation; negative regulation of osteoblast differentiation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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