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FUT9 siRNA – Alpha-(1,3)-Fucosyltransferase 9 (Mouse)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8229705

MBS8229705 is an siRNA targeting FUT9. Also known as FUT9 siRNA (Mouse). Reactive in Mouse. Host: Synthetic. siRNA to inhibit FUT9 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
FUT9, siRNA
Full Product Name
FUT9 siRNA (Mouse)
Product Gene Name
FUT9 sirna
Product Synonym Names
Alpha-(1.3)-fucosyltransferase 9; Fucosyltransferase 9; Fucosyltransferase IX; Fuc-TIX; FucT-IX; Galactoside 3-L-fucosyltransferase
Host
Synthetic
Species Reactivity
Mouse
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
FUT9 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
42,041 Da
3D Structure
ModBase 3D Structure for O88819
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse FUT9 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for FUT9 sirna
RNA Interference (RNAi)

Other Notes

Small volumes of FUT9 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for FUT9. It may not necessarily be applicable to this product.

NCBI GI #
6753922
NCBI GeneID
14348
NCBI Accession #
NP_034373.1
UniProt Primary Accession #
O88819
UniProt Secondary Accession #
Q3TQ63
UniProt Related Accession #
O88819
GenBank Nucleotide #
NM_010243.3
NCBI Official Full Name
alpha-(1,3)-fucosyltransferase 9
NCBI Official Synonym Full Names
fucosyltransferase 9
NCBI Official Symbol
Fut9
NCBI Official Synonym Symbols
mFUT9; AI746471; AU067636; mFuc-TIX
UniProt Protein Name
Alpha-(1,3)-fucosyltransferase 9
UniProt Synonym Protein Names
Fucosyltransferase 9; Fucosyltransferase IX; Fuc-TIX; FucT-IX; Galactoside 3-L-fucosyltransferase
Protein Family
Alpha-(1,3)-fucosyltransferase
UniProt Gene Name
Fut9
UniProt Synonym Gene Names
Fuc-TIX; FucT-IX
UniProt Entry Name
FUT9_MOUSE
NCBI Protein Information
alpha-(1,3)-fucosyltransferase 9

UniProt Comments for FUT9

FUT9: Transfers a fucose to lacto-N-neotetraose but not to either alpha2,3-sialyl lacto-N-neotetraose or lacto-N-tetraose. Can catalyze the last step in the biosynthesis of Lewis antigen, the addition of a fucose to precursor polysaccharides. Belongs to the glycosyltransferase 10 family.

Protein type: Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series; Glycan Metabolism - glycosphingolipid biosynthesis - globo series; Membrane protein, integral; Transferase; EC 2.4.1.-

Cellular Component: Golgi apparatus; membrane; integral to membrane

Molecular Function: acetylgalactosaminyltransferase activity; alpha(1,6)-fucosyltransferase activity; oligosaccharyl transferase activity; alpha(1,3)-fucosyltransferase activity; glucosyltransferase activity; O antigen polymerase activity; alpha-1,2-mannosyltransferase activity; acetylglucosaminyltransferase activity; transferase activity, transferring glycosyl groups; 1-phenanthrol glycosyltransferase activity; phenanthrol glycosyltransferase activity; glycolipid mannosyltransferase activity; galactosyltransferase activity; dolichyl-phosphate-glucose-glycolipid alpha-glucosyltransferase activity; dolichyl pyrophosphate Man7GlcNAc2 alpha-1,3-glucosyltransferase activity; fucosyltransferase activity; lipopolysaccharide-1,6-galactosyltransferase activity; alpha-1,2-galactosyltransferase activity; alpha-1,3-galactosyltransferase activity; dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase activity; 9-phenanthrol UDP-glucuronosyltransferase activity; UDP-glucose:glycoprotein glucosyltransferase activity; 1,2-dihydroxy-phenanthrene glycosyltransferase activity; transferase activity; 9-phenanthrol glycosyltransferase activity; dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase activity; beta-1,4-mannosyltransferase activity; cellulose synthase activity; mannosyltransferase activity; alpha-1,3-mannosyltransferase activity; alpha-1,6-mannosyltransferase activity; inositol phosphoceramide synthase activity

Biological Process: nervous system development; protein amino acid glycosylation
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Research Articles on FUT9

1. Mice lacking alpha1,3-fucosyltransferase 9 exhibit modulation of in vivo immune responses against pathogens.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with FUT9 sirna

 Reference Product  PubMed Publications
 Fut1 sirna  >3 publications with FUT9 and Fut1
 Fut2 sirna  >2 publications with FUT9 and Fut2
 Ggta1 sirna  >1 publications with FUT9 and Ggta1
 B4galt1 sirna  >1 publications with FUT9 and B4galt1
 St3gal6 sirna  >1 publications with FUT9 and St3gal6

Pathways associated with FUT9 sirna

 Products by Pathway  Pathway Diagram
 Glycosphingolipid Biosynthesis - Globo Series Pathway antibodies  Glycosphingolipid Biosynthesis - Globo Series Pathway Diagram
 Glycosphingolipid Biosynthesis - Globo Series Pathway antibodies  Glycosphingolipid Biosynthesis - Globo Series Pathway Diagram
 Glycosphingolipid Biosynthesis - Lacto And Neolacto Series Pathway antibodies  Glycosphingolipid Biosynthesis - Lacto And Neolacto Series Pathway Diagram
 Glycosphingolipid Biosynthesis - Lacto And Neolacto Series Pathway antibodies  Glycosphingolipid Biosynthesis - Lacto And Neolacto Series Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Other Types Of O-glycan Biosynthesis Pathway antibodies  Other Types Of O-glycan Biosynthesis Pathway Diagram
 Other Types Of O-glycan Biosynthesis Pathway antibodies  Other Types Of O-glycan Biosynthesis Pathway Diagram

Diseases associated with FUT9 sirna

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >3 publications with FUT9 and Inflammation
 Ovarian Neoplasms Antibodies  >1 publications with FUT9 and Ovarian Neoplasms
 Memory Disorders Antibodies  >1 publications with FUT9 and Memory Disorders
 Learning Disorders Antibodies  >1 publications with FUT9 and Learning Disorders
 Weight Gain Antibodies  >1 publications with FUT9 and Weight Gain
 Ovarian Diseases Antibodies  >1 publications with FUT9 and Ovarian Diseases

Organs/Tissues associated with FUT9 sirna

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >9 publications with FUT9 and Brain
 Embryonic Tissue Antibodies  >3 publications with FUT9 and Embryonic Tissue
 Eye Antibodies  >2 publications with FUT9 and Eye
 Spleen Antibodies  >2 publications with FUT9 and Spleen
 Kidney Antibodies  >2 publications with FUT9 and Kidney
 Uterus Antibodies  >1 publications with FUT9 and Uterus
 Prostate Antibodies  >1 publications with FUT9 and Prostate
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