GCLC siRNA – Glutamate--Cysteine Ligase Catalytic Subunit (Mouse)

Name: GCLC siRNA; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS8227163
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8227163

MBS8227163 is an siRNA targeting GCLC. Also known as GCLC siRNA (Mouse). Reactive in Mouse. Host: Synthetic. siRNA to inhibit GCLC expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

GCLC, siRNA

Full Product Name

GCLC siRNA (Mouse)

Product Gene Name

GCLC sirna

Product Synonym Names

GLCLC; Glutamate--cysteine ligase catalytic subunit; GCS heavy chain; Gamma-ECS; Gamma-glutamylcysteine synthetase

Host

Synthetic

Species Reactivity

Mouse

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

GCLC siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

72,571 Da

3D Structure

ModBase 3D Structure for P97494

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse GCLC gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for GCLC sirna

RNA Interference (RNAi)

Other Notes

Small volumes of GCLC sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GCLC. It may not necessarily be applicable to this product.

NCBI GI #

33468897

NCBI GeneID

14629

NCBI Accession #

NP_034425.1

UniProt Primary Accession #

P97494

UniProt Secondary Accession #

O09166; Q8VCS5

UniProt Related Accession #

P97494

GenBank Nucleotide #

NM_010295.2

NCBI Official Full Name

glutamate--cysteine ligase catalytic subunit

NCBI Official Synonym Full Names

glutamate-cysteine ligase, catalytic subunit

NCBI Official Symbol

Gclc

NCBI Official Synonym Symbols

Glclc; GLCL-H; Ggcs-hs; D9Wsu168e

UniProt Protein Name

Glutamate--cysteine ligase catalytic subunit

UniProt Synonym Protein Names

GCS heavy chain; Gamma-ECS; Gamma-glutamylcysteine synthetase

Protein Family

Glutamate--cysteine ligase

UniProt Gene Name

Gclc

UniProt Synonym Gene Names

Glclc

UniProt Entry Name

GSH1_MOUSE

NCBI Protein Information

glutamate--cysteine ligase catalytic subunit

UniProt Comments for GCLC

GCLC: Defects in GCLC are the cause of hemolytic anemia (HAGGSD). Belongs to the glutamate--cysteine ligase type 3 family.

Protein type: Other Amino Acids Metabolism - glutathione; EC 6.3.2.2; Ligase

Cellular Component: glutamate-cysteine ligase complex; cytosol

Molecular Function: glutamate binding; protein heterodimerization activity; glutamate-cysteine ligase activity; nucleotide binding; magnesium ion binding; coenzyme binding; ADP binding; ATP binding; ligase activity

Biological Process: glutamate metabolic process; response to arsenic; L-ascorbic acid metabolic process; response to hormone stimulus; response to xenobiotic stimulus; response to nitrosative stress; cysteine metabolic process; apoptotic mitochondrial changes; glutathione metabolic process; response to heat; cell redox homeostasis; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; glutathione biosynthetic process; negative regulation of protein ubiquitination; response to oxidative stress; negative regulation of neuron apoptosis; regulation of mitochondrial depolarization; negative regulation of transcription, DNA-dependent; regulation of blood vessel size; negative regulation of apoptosis

Research Articles on GCLC

1. A floxed Gclc mouse was generated and crossed with a transgenic mouse expressing Cre in the lens to generate the Lens Glutathione Synthesis Knockout mouse in which de novo GSH synthesis was completely abolished in the lens.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GCLC sirna

Reference Product	PubMed Publications
Gclm sirna	>188 publications with GCLC and Gclm
Gss sirna	>15 publications with GCLC and Gss
Mafg sirna	>4 publications with GCLC and Mafg
Ggt1 sirna	>1 publications with GCLC and Ggt1

Pathways associated with GCLC sirna

Products by Pathway	Pathway Diagram
Biological Oxidations Pathway antibodies	Biological Oxidations Pathway Diagram
Glutathione And One Carbon Metabolism Pathway antibodies	Glutathione And One Carbon Metabolism Pathway Diagram
Glutathione Biosynthesis, Glutamate => Glutathione Pathway antibodies	Glutathione Biosynthesis, Glutamate => Glutathione Pathway Diagram
Glutathione Biosynthesis, Glutamate => Glutathione Pathway antibodies	Glutathione Biosynthesis, Glutamate => Glutathione Pathway Diagram
Glutathione Conjugation Pathway antibodies	Glutathione Conjugation Pathway Diagram
Glutathione Metabolism Pathway antibodies	Glutathione Metabolism Pathway Diagram
Glutathione Metabolism Pathway antibodies	Glutathione Metabolism Pathway Diagram
Glutathione Metabolism Pathway antibodies	Glutathione Metabolism Pathway Diagram
Glutathione Synthesis And Recycling Pathway antibodies	Glutathione Synthesis And Recycling Pathway Diagram
Keap1-Nrf2 Pathway antibodies	Keap1-Nrf2 Pathway Diagram

Diseases associated with GCLC sirna

Disease Name	Pubmed Publications
Liver Diseases Antibodies	>52 publications with GCLC and Liver Diseases
Necrosis Antibodies	>35 publications with GCLC and Necrosis
Disease Models, Animal Antibodies	>32 publications with GCLC and Disease Models, Animal
Inflammation Antibodies	>31 publications with GCLC and Inflammation
Cardiovascular Diseases Antibodies	>30 publications with GCLC and Cardiovascular Diseases
Drug-Induced Liver Injury Antibodies	>22 publications with GCLC and Drug-Induced Liver Injury
Nervous System Diseases Antibodies	>20 publications with GCLC and Nervous System Diseases
Poisoning Antibodies	>17 publications with GCLC and Poisoning
Fibrosis Antibodies	>14 publications with GCLC and Fibrosis
Heart Diseases Antibodies	>13 publications with GCLC and Heart Diseases

Organs/Tissues associated with GCLC sirna

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>112 publications with GCLC and Liver
Blood Antibodies	>73 publications with GCLC and Blood
Lung Antibodies	>55 publications with GCLC and Lung
Brain Antibodies	>47 publications with GCLC and Brain
Kidney Antibodies	>25 publications with GCLC and Kidney
Heart Antibodies	>19 publications with GCLC and Heart
Muscle Antibodies	>18 publications with GCLC and Muscle
Skin Antibodies	>14 publications with GCLC and Skin
Eye Antibodies	>9 publications with GCLC and Eye
Intestine Antibodies	>9 publications with GCLC and Intestine