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GZMK siRNA – Granzyme K (Mouse)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8205969

MBS8205969 is an siRNA targeting GZMK. Also known as GZMK siRNA (Mouse). Reactive in Mouse. Host: Synthetic. siRNA to inhibit GZMK expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
PDF
Testing Data View PDF
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Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GZMK, siRNA
Full Product Name
GZMK siRNA (Mouse)
Product Gene Name
GZMK sirna
Product Synonym Names
Granzyme K
Host
Synthetic
Species Reactivity
Mouse
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
GZMK siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
29,251 Da
3D Structure
ModBase 3D Structure for O35205
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse GZMK gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for GZMK sirna
RNA Interference (RNAi)

Other Notes

Small volumes of GZMK sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GZMK. It may not necessarily be applicable to this product.

NCBI GI #
6680143
NCBI GeneID
14945
NCBI Accession #
NP_032222.1
UniProt Primary Accession #
O35205
UniProt Related Accession #
O35205
GenBank Nucleotide #
NM_008196.1
NCBI Official Full Name
granzyme K
NCBI Official Synonym Full Names
granzyme K
NCBI Official Symbol
Gzmk
UniProt Protein Name
Granzyme K
Protein Family
Granzyme
UniProt Gene Name
Gzmk
UniProt Entry Name
GRAK_MOUSE
NCBI Protein Information
granzyme K

UniProt Comments for GZMK

GZMK: this protein is a member of a group of related serine proteases from the cytoplasmic granules of cytotoxic lymphocytes. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here lacks consensus sequences for N-glycosylation present in other granzymes. [provided by RefSeq, Jul 2008]

Protein type: Secreted; Secreted, signal peptide; EC 3.4.21.-; Protease

Molecular Function: peptidase activity; hydrolase activity; serine-type peptidase activity; serine-type endopeptidase activity; catalytic activity

Biological Process: proteolysis
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Research Articles on GZMK

1. The data suggest that T-killer cells control lymphocytic choriomeningitis virus through non-cytotoxic processes that involve gzmK.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GZMK sirna

 Reference Product  PubMed Publications
 Gzma sirna  >13 publications with GZMK and Gzma
 Set sirna  >3 publications with GZMK and Set
 Prf1 sirna  >1 publications with GZMK and Prf1
 Vcp sirna  >1 publications with GZMK and Vcp

Diseases associated with GZMK sirna

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >4 publications with GZMK and Inflammation
 Heart Diseases Antibodies  >1 publications with GZMK and Heart Diseases
 Depressive Disorder Antibodies  >1 publications with GZMK and Depressive Disorder
 Poisoning Antibodies  >1 publications with GZMK and Poisoning
 Lung Diseases Antibodies  >1 publications with GZMK and Lung Diseases
 Disease Progression Antibodies  >1 publications with GZMK and Disease Progression
 Pneumonia Antibodies  >1 publications with GZMK and Pneumonia
 Necrosis Antibodies  >1 publications with GZMK and Necrosis
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