IL21 siRNA – Interleukin-21 Isoform 2 (Mouse)

Name: IL21 siRNA; Interleukin-21 Isoform 2; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS8206149
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8206149

MBS8206149 is an siRNA targeting IL21. Also known as IL21 siRNA (Mouse). Reactive in Mouse. Host: Synthetic. siRNA to inhibit IL21 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

IL21, siRNA

Full Product Name

IL21 siRNA (Mouse)

Product Gene Name

IL21 sirna

Product Synonym Names

Interleukin-21; IL-21

Host

Synthetic

Species Reactivity

Mouse

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

IL21 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

16,811 Da

3D Structure

ModBase 3D Structure for Q9ES17

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse IL21 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for IL21 sirna

RNA Interference (RNAi)

Other Notes

Small volumes of IL21 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for IL21. It may not necessarily be applicable to this product.

NCBI GI #

11140821

NCBI GeneID

60505

NCBI Accession #

NP_068554.1

UniProt Primary Accession #

Q9ES17

UniProt Related Accession #

Q9ES17

GenBank Nucleotide #

NM_021782.3

NCBI Official Full Name

interleukin-21 isoform 2

NCBI Official Synonym Full Names

interleukin 21

NCBI Official Symbol

Il21

NCBI Official Synonym Symbols

IL-21

UniProt Protein Name

Interleukin-21

Protein Family

Interleukin

UniProt Gene Name

Il21

UniProt Synonym Gene Names

IL-21

UniProt Entry Name

IL21_MOUSE

NCBI Protein Information

interleukin-21

UniProt Comments for IL21

IL21: Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells. May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation. Belongs to the IL-15/IL-21 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; Secreted, signal peptide; Secreted; Cytokine; Apoptosis

Cellular Component: extracellular space; extracellular region

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor binding; interleukin-2 receptor binding; cytokine activity

Biological Process: positive regulation of interleukin-17 production; positive regulation of natural killer cell cytokine production; cell maturation; B cell apoptosis; positive regulation of natural killer cell mediated cytotoxicity; positive regulation of interleukin-10 production; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of natural killer cell differentiation; positive regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of cell proliferation; positive regulation of B cell proliferation; positive regulation of B cell differentiation; immune response; positive regulation of T cell proliferation; positive regulation of interferon-gamma biosynthetic process

Research Articles on IL21

1. Data indicate that interleukin 21 knockout (il-21-/-) mice showed significantly diminished T follicular helper cell (TFH) generation/accumulation compared to wild type mice.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with IL21 sirna

Reference Product	PubMed Publications
Il21r sirna	>402 publications with IL21 and Il21r
Il15 sirna	>84 publications with IL21 and Il15
Il4 sirna	>16 publications with IL21 and Il4
Il2rb sirna	>11 publications with IL21 and Il2rb
Il13 sirna	>6 publications with IL21 and Il13
Il7 sirna	>5 publications with IL21 and Il7
Il5 sirna	>4 publications with IL21 and Il5

Pathways associated with IL21 sirna

Products by Pathway	Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Inflammatory Bowel Disease (IBD) Pathway antibodies	Inflammatory Bowel Disease (IBD) Pathway Diagram
Inflammatory Bowel Disease (IBD) Pathway antibodies	Inflammatory Bowel Disease (IBD) Pathway Diagram
Jak-STAT Signaling Pathway antibodies	Jak-STAT Signaling Pathway Diagram
Jak-STAT Signaling Pathway antibodies	Jak-STAT Signaling Pathway Diagram

Diseases associated with IL21 sirna

Disease Name	Pubmed Publications
Immune System Diseases Antibodies	>400 publications with IL21 and Immune System Diseases
Autoimmune Diseases Antibodies	>286 publications with IL21 and Autoimmune Diseases
Inflammation Antibodies	>197 publications with IL21 and Inflammation
Disease Models, Animal Antibodies	>128 publications with IL21 and Disease Models, Animal
Lupus Erythematosus, Systemic Antibodies	>74 publications with IL21 and Lupus Erythematosus, Systemic
Necrosis Antibodies	>72 publications with IL21 and Necrosis
Liver Diseases Antibodies	>51 publications with IL21 and Liver Diseases
Neoplasms, Experimental Antibodies	>40 publications with IL21 and Neoplasms, Experimental
Demyelinating Diseases Antibodies	>39 publications with IL21 and Demyelinating Diseases
Hypersensitivity Antibodies	>32 publications with IL21 and Hypersensitivity

Organs/Tissues associated with IL21 sirna

Organ/Tissue Name	Pubmed Publications
Spleen Antibodies	>80 publications with IL21 and Spleen
Thymus Antibodies	>9 publications with IL21 and Thymus