ITPKC siRNA – Inositol-Trisphosphate 3-Kinase C (Mouse)

Name: ITPKC siRNA; Inositol-Trisphosphate 3-Kinase C; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS8212728
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8212728

MBS8212728 is an siRNA targeting ITPKC. Also known as ITPKC siRNA (Mouse). Reactive in Mouse. Host: Synthetic. siRNA to inhibit ITPKC expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

ITPKC, siRNA

Full Product Name

ITPKC siRNA (Mouse)

Product Gene Name

ITPKC sirna

Product Synonym Names

Inositol-trisphosphate 3-kinase C; Inositol 1.4.5-trisphosphate 3-kinase C; IP3 3-kinase C; IP3K C; InsP 3-kinase C

Host

Synthetic

Species Reactivity

Mouse

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

ITPKC siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

74,493 Da

3D Structure

ModBase 3D Structure for Q7TS72

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ITPKC gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for ITPKC sirna

RNA Interference (RNAi)

Other Notes

Small volumes of ITPKC sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ITPKC. It may not necessarily be applicable to this product.

NCBI GI #

31712006

NCBI GeneID

233011

NCBI Accession #

NP_853624.1

UniProt Primary Accession #

Q7TS72

UniProt Secondary Accession #

Q3U384

UniProt Related Accession #

Q7TS72

GenBank Nucleotide #

NM_181593.2

NCBI Official Full Name

inositol-trisphosphate 3-kinase C

NCBI Official Synonym Full Names

inositol 1,4,5-trisphosphate 3-kinase C

NCBI Official Symbol

Itpkc

NCBI Official Synonym Symbols

9130023N17Rik

UniProt Protein Name

Inositol-trisphosphate 3-kinase C

UniProt Synonym Protein Names

Inositol 1,4,5-trisphosphate 3-kinase C; IP3 3-kinase C; IP3K C; InsP 3-kinase C

Protein Family

Inositol-trisphosphate 3-kinase

UniProt Gene Name

Itpkc

UniProt Synonym Gene Names

IP3 3-kinase C; IP3K C; InsP 3-kinase C

UniProt Entry Name

IP3KC_MOUSE

NCBI Protein Information

inositol-trisphosphate 3-kinase C

UniProt Comments for ITPKC

ITPKC: Can phosphorylate inositol 2,4,5-triphosphate to inositol 2,4,5,6-tetraphosphate. Defects in ITPKC are a cause of Kawasaki disease (KWD); also known as mucocutaneous lymph node syndrome or infantile polyarteritis. Kawasaki disease is an acute, self- limited vasculitis of infants and children characterized by prolonged fever unresponsive to antibiotics, polymorphous skin rash, erythema of the oral mucosa, lips, and tongue, erythema of the palms and soles, bilateral conjunctival injection, and cervical lymphadenopathy. Coronary artery aneurysms develop in 15 to 25% of those left untreated, making Kawasaki disease the leading cause of acquired heart disease among children in developed countries. Belongs to the inositol phosphokinase (IPK) family.

Protein type: EC 2.7.1.127; Kinase, lipid; Motility/polarity/chemotaxis

Cellular Component: cytoplasm; nucleus

Molecular Function: transferase activity; calmodulin binding; protein binding; nucleotide binding; kinase activity; inositol trisphosphate 3-kinase activity; ATP binding

Biological Process: phosphorylation

Research Articles on ITPKC

1. These data show that the absence of expression of the three isoenzymes of Itpk does not prevent the formation of IP5 and IP6, at least in mouse embryonic fibroblasts.[Itpka, Itpkb, Itpkc]

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ITPKC sirna

Reference Product	PubMed Publications
Ipmk sirna	>2 publications with ITPKC and Ipmk

Pathways associated with ITPKC sirna

Products by Pathway	Pathway Diagram
1D-myo-inositol Hexakisphosphate Biosynthesis II (mammalian) Pathway antibodies	1D-myo-inositol Hexakisphosphate Biosynthesis II (mammalian) Pathway Diagram
Calcium Signaling Pathway antibodies	Calcium Signaling Pathway Diagram
Calcium Signaling Pathway antibodies	Calcium Signaling Pathway Diagram
D-myo-inositol (1,3,4)-trisphosphate Biosynthesis Pathway antibodies	D-myo-inositol (1,3,4)-trisphosphate Biosynthesis Pathway Diagram
Inositol Phosphate Metabolism Pathway antibodies	Inositol Phosphate Metabolism Pathway Diagram
Inositol Phosphate Metabolism Pathway antibodies	Inositol Phosphate Metabolism Pathway Diagram
Inositol Phosphate Metabolism Pathway antibodies	Inositol Phosphate Metabolism Pathway Diagram
Inositol Phosphate Metabolism, PI=> PIP2 => Ins(1,4,5)P3 => Ins(1,3,4,5)P4 Pathway antibodies	Inositol Phosphate Metabolism, PI=> PIP2 => Ins(1,4,5)P3 => Ins(1,3,4,5)P4 Pathway Diagram
Inositol Phosphate Metabolism, PI=> PIP2 => Ins(1,4,5)P3 => Ins(1,3,4,5)P4 Pathway antibodies	Inositol Phosphate Metabolism, PI=> PIP2 => Ins(1,4,5)P3 => Ins(1,3,4,5)P4 Pathway Diagram
Metabolic Pathways antibodies	Metabolic Pathways Diagram

Diseases associated with ITPKC sirna

Disease Name	Pubmed Publications
Mucocutaneous Lymph Node Syndrome Antibodies	>21 publications with ITPKC and Mucocutaneous Lymph Node Syndrome
Inflammation Antibodies	>3 publications with ITPKC and Inflammation
Coronary Aneurysm Antibodies	>3 publications with ITPKC and Coronary Aneurysm
Liver Neoplasms Antibodies	>1 publications with ITPKC and Liver Neoplasms
Neurotoxicity Syndromes Antibodies	>1 publications with ITPKC and Neurotoxicity Syndromes
Hypertension Antibodies	>1 publications with ITPKC and Hypertension
Fibrosis Antibodies	>1 publications with ITPKC and Fibrosis
Liver Diseases Antibodies	>1 publications with ITPKC and Liver Diseases
Kidney Diseases Antibodies	>1 publications with ITPKC and Kidney Diseases
Poisoning Antibodies	>1 publications with ITPKC and Poisoning

Organs/Tissues associated with ITPKC sirna

Organ/Tissue Name	Pubmed Publications
Connective Tissue Antibodies	>1 publications with ITPKC and Connective Tissue
Muscle Antibodies	>1 publications with ITPKC and Muscle
Eye Antibodies	>1 publications with ITPKC and Eye