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LIPT1 siRNA – Lipoyltransferase 1, Mitochondrial (Mouse)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8204269

MBS8204269 is an siRNA targeting LIPT1. Also known as LIPT1 siRNA (Mouse). Reactive in Mouse. Host: Synthetic. siRNA to inhibit LIPT1 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
LIPT1, siRNA
Full Product Name
LIPT1 siRNA (Mouse)
Product Gene Name
LIPT1 sirna
Product Synonym Names
Lipoyltransferase 1 mitochondrial; Lipoate biosynthesis protein; Lipoate-protein ligase; Lipoyl ligase
Host
Synthetic
Species Reactivity
Mouse
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
LIPT1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
42,146 Da
3D Structure
ModBase 3D Structure for Q8VCM4
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse LIPT1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for LIPT1 sirna
RNA Interference (RNAi)

Other Notes

Small volumes of LIPT1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for LIPT1. It may not necessarily be applicable to this product.

NCBI GI #
158853994
NCBI GeneID
623661
NCBI Accession #
NP_001033007.2
UniProt Primary Accession #
Q8VCM4
UniProt Related Accession #
Q8VCM4
GenBank Nucleotide #
NM_001037918.3
NCBI Official Full Name
lipoyltransferase 1, mitochondrial
NCBI Official Synonym Full Names
lipoyltransferase 1
NCBI Official Symbol
Lipt1
NCBI Official Synonym Symbols
EG623661
UniProt Protein Name
Lipoyltransferase 1, mitochondrial
UniProt Synonym Protein Names
Lipoate biosynthesis protein; Lipoate-protein ligase; Lipoyl ligase
Protein Family
Lipoyltransferase
UniProt Gene Name
Lipt1
UniProt Entry Name
LIPT_MOUSE
NCBI Protein Information
lipoyltransferase 1, mitochondrial

UniProt Comments for LIPT1

LIPT1: Catalyzes the transfer of the lipoyl group from lipoyl- AMP to the specific lysine residue of lipoyl domains of lipoate- dependent enzymes. Belongs to the LplA family.

Protein type: EC 2.3.1.-; Transferase; Cofactor and Vitamin Metabolism - lipoic acid

Cellular Component: mitochondrion

Molecular Function: keto acid formate lyase activity; glucosaminyl-phosphotidylinositol O-acyltransferase activity; S-malonyltransferase activity; 3-hydroxybutyryl-CoA thiolase activity; S-acetyltransferase activity; O-octanoyltransferase activity; O-sinapoyltransferase activity; acyl-CoA N-acyltransferase activity; palmitoleoyl [acyl-carrier-protein]-dependent acyltransferase activity; peptidyl-lysine N6-myristoyltransferase activity; peptidyl-lysine N6-palmitoyltransferase activity; azetidine-2-carboxylic acid acetyltransferase activity; succinyltransferase activity; dihydrolipoamide branched chain acyltransferase activity; Ras palmitoyltransferase activity; palmitoyltransferase activity; C-acyltransferase activity; C-palmitoyltransferase activity; dihydrolipoamide S-acyltransferase activity; protein-cysteine S-acyltransferase activity; N-acetyltransferase activity; serine O-acyltransferase activity; O-succinyltransferase activity; malonyltransferase activity; O-palmitoyltransferase activity; octanoyltransferase activity; sterol O-acyltransferase activity; 3-ketopimelyl-CoA thiolase activity; carnitine O-acyltransferase activity; S-succinyltransferase activity; N-acyltransferase activity; UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase activity; N-palmitoyltransferase activity; O-acyltransferase activity; acylglycerol O-acyltransferase activity; O-acetyltransferase activity; benzoyl acetate-CoA thiolase activity; transferase activity; sinapoyltransferase activity; protein-cysteine S-myristoyltransferase activity; S-acyltransferase activity; N-succinyltransferase activity; myristoyltransferase activity; acetyltransferase activity; transferase activity, transferring acyl groups; L-2-aminoadipate N-acetyltransferase activity

Biological Process: protein lipoylation; protein modification process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with LIPT1 sirna

 Reference Product  PubMed Publications
 Lias sirna  >3 publications with LIPT1 and Lias
 Dld sirna  >2 publications with LIPT1 and Dld
 Lipt2 sirna  >1 publications with LIPT1 and Lipt2

Pathways associated with LIPT1 sirna

 Products by Pathway  Pathway Diagram
 Lipoic Acid Metabolism Pathway antibodies  Lipoic Acid Metabolism Pathway Diagram
 Lipoic Acid Metabolism Pathway antibodies  Lipoic Acid Metabolism Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram

Diseases associated with LIPT1 sirna

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >1 publications with LIPT1 and Nervous System Diseases
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