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MACROD1 siRNA – O-Acetyl-ADP-Ribose Deacetylase MACROD1 (Mouse)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8230593

MBS8230593 is an siRNA targeting MACROD1. Also known as MACROD1 siRNA (Mouse). Reactive in Mouse. Host: Synthetic. siRNA to inhibit MACROD1 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MACROD1, siRNA
Full Product Name
MACROD1 siRNA (Mouse)
Product Gene Name
MACROD1 sirna
Product Synonym Names
LRP16; O-acetyl-ADP-ribose deacetylase MACROD1; MACRO domain-containing protein 1; Protein LRP16; [Protein ADP-ribosylglutamate] hydrolase
Host
Synthetic
Species Reactivity
Mouse
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
MACROD1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
35,295 Da
3D Structure
ModBase 3D Structure for Q922B1
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse MACROD1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for MACROD1 sirna
RNA Interference (RNAi)

Other Notes

Small volumes of MACROD1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MACROD1. It may not necessarily be applicable to this product.

NCBI GI #
170650601
NCBI GeneID
107227
NCBI Accession #
NP_598908.2
UniProt Primary Accession #
Q922B1
UniProt Related Accession #
Q922B1
GenBank Nucleotide #
NM_134147.4
NCBI Official Full Name
O-acetyl-ADP-ribose deacetylase MACROD1
NCBI Official Synonym Full Names
MACRO domain containing 1
NCBI Official Symbol
Macrod1
NCBI Official Synonym Symbols
Lrp16; AI604841; AW743046; D930010J01Rik
UniProt Protein Name
O-acetyl-ADP-ribose deacetylase MACROD1
UniProt Synonym Protein Names
MACRO domain-containing protein 1; Protein LRP16; [Protein ADP-ribosylglutamate] hydrolase
Protein Family
O-acetyl-ADP-ribose deacetylase
UniProt Gene Name
Macrod1
UniProt Synonym Gene Names
Lrp16
UniProt Entry Name
MACD1_MOUSE
NCBI Protein Information
O-acetyl-ADP-ribose deacetylase MACROD1

UniProt Comments for MACROD1

MACROD1: Deacetylates O-acetyl-ADP ribose, a signaling molecule generated by the deacetylation of acetylated lysine residues in histones and other proteins. Plays a role in estrogen signaling. Binds to androgen receptor (AR) and amplifies the transactivation function of AR in response to androgen. May play an important role in carcinogenesis and/or progression of hormone-dependent cancers by feed-forward mechanism that activates ESR1 transactivation. Could be an ESR1 coactivator, providing a positive feedback regulatory loop for ESR1 signal transduction. Could be involved in invasive growth by down-regulating CDH1 in endometrial cancer cells. Enhances ESR1-mediated transcription activity. A chromosomal aberration involving MACROD1 is found in acute leukemia. Translocation t(11;21)(q13;q22) that forms a RUNX1-MACROD1 fusion protein.

Protein type: EC 3.5.1.-; Mitochondrial

Cellular Component: mitochondrion; nucleus

Molecular Function: (3,5-dichlorophenylurea)acetate amidohydrolase activity; single-strand selective uracil DNA N-glycosylase activity; deacetylase activity; uracil DNA N-glycosylase activity; hydrolase activity; DNA N-glycosylase activity; hydrolase activity, acting on glycosyl bonds; purine-specific mismatch base pair DNA N-glycosylase activity; UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase activity; N-acetylgalactosamine-6-phosphate deacetylase activity; N2-acetyl-L-lysine deacetylase activity; DNA-3-methyladenine glycosylase III activity; iprodione amidohydrolase activity; indoleacetamide hydrolase activity; protein N-terminal asparagine amidohydrolase activity; histone deacetylase activity; O-succinylbenzoate synthase activity

Biological Process: protein amino acid de-ADP-ribosylation; purine nucleoside metabolic process; response to DNA damage stimulus
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Research Articles on MACROD1

1. These results suggest that LRP16 plays a key role in maintaining pancreatic beta-cell functions and may help us to understand the protective effects of estrogen on the functions of pancreatic beta-cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with MACROD1 sirna

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >1 publications with MACROD1 and Inflammation

Organs/Tissues associated with MACROD1 sirna

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >1 publications with MACROD1 and Liver
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