• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

MAPK6 siRNA – Mitogen-Activated Protein Kinase 6 (Mouse)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS828957

MBS828957 is an siRNA targeting MAPK6. Also known as MAPK6 siRNA (Mouse). Reactive in Mouse. Host: Synthetic. siRNA to inhibit MAPK6 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MAPK6, siRNA
Full Product Name
MAPK6 siRNA (Mouse)
Product Gene Name
MAPK6 sirna
Product Synonym Names
ERK3; PRKM4; PRKM6; Mitogen-activated protein kinase 6; MAP kinase 6; MAPK 6; Extracellular signal-regulated kinase 3; ERK-3
Host
Synthetic
Species Reactivity
Mouse
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
MAPK6 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
82,199 Da
3D Structure
ModBase 3D Structure for Q61532
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse MAPK6 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for MAPK6 sirna
RNA Interference (RNAi)

Other Notes

Small volumes of MAPK6 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MAPK6. It may not necessarily be applicable to this product.

NCBI GI #
76573879
NCBI GeneID
50772
NCBI Accession #
NP_056621.4
UniProt Primary Accession #
Q61532
UniProt Secondary Accession #
Q497T9; Q6YKB0; Q7TT30; Q80XH5; Q922U4; Q9JLU6
UniProt Related Accession #
Q61532
GenBank Nucleotide #
NM_015806.5
NCBI Official Full Name
mitogen-activated protein kinase 6
NCBI Official Synonym Full Names
mitogen-activated protein kinase 6
NCBI Official Symbol
Mapk6
NCBI Official Synonym Symbols
Erk3; Mapk4; Prkm4; Prkm6; Mapk63; 2610021I23Rik; D130053K17Rik
UniProt Protein Name
Mitogen-activated protein kinase 6
UniProt Synonym Protein Names
Extracellular signal-regulated kinase 3; ERK-3
UniProt Gene Name
Mapk6
UniProt Synonym Gene Names
Erk3; Prkm4; Prkm6; MAP kinase 6; MAPK 6; ERK-3
UniProt Entry Name
MK06_MOUSE
NCBI Protein Information
mitogen-activated protein kinase 6

UniProt Comments for MAPK6

ERK3: a protein kinase of the MAPK family. Plays a critical role in the regulation of cell growth and differentiation. Localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters.

Protein type: EC 2.7.11.24; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, CMGC; CMGC group; MAPK family; ERK subfamily; MAPK/ERK subfamily

Cellular Component: cytoplasm; nucleus

Molecular Function: transferase activity; MAP kinase activity; protein serine/threonine kinase activity; protein binding; protein heterodimerization activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; protein kinase binding; ATP binding; protein kinase activity

Biological Process: MAPKKK cascade; cell cycle; phosphorylation; protein amino acid phosphorylation
Show more

Research Articles on MAPK6

1. Results show that Cdo is important for full Abl kinase activity, and Abl is necessary for full activation of p38 MAPK, during myogenic differentiation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with MAPK6 sirna

 Reference Product  PubMed Publications
 Jun sirna  >23 publications with MAPK6 and Jun
 Mapkapk5 sirna  >10 publications with MAPK6 and Mapkapk5
 Cdc14a sirna  >2 publications with MAPK6 and Cdc14a

Pathways associated with MAPK6 sirna

 Products by Pathway  Pathway Diagram
 ESC Pluripotency Pathways antibodies  ESC Pluripotency Pathways Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Insulin Signaling Pathway antibodies  Insulin Signaling Pathway Diagram
 Integrin-mediated Cell Adhesion Pathway antibodies  Integrin-mediated Cell Adhesion Pathway Diagram
 MAPK Family Signaling Cascades Pathway antibodies  MAPK Family Signaling Cascades Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK6/MAPK4 Signaling Pathway antibodies  MAPK6/MAPK4 Signaling Pathway Diagram
 Regulation Of Actin Cytoskeleton Pathway antibodies  Regulation Of Actin Cytoskeleton Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signal Transduction Of S1P Receptor Pathway antibodies  Signal Transduction Of S1P Receptor Pathway Diagram

Diseases associated with MAPK6 sirna

 Disease Name  Pubmed Publications
 Death Antibodies  >12 publications with MAPK6 and Death
 Carcinoma Antibodies  >9 publications with MAPK6 and Carcinoma
 Disease Models, Animal Antibodies  >8 publications with MAPK6 and Disease Models, Animal
 Necrosis Antibodies  >6 publications with MAPK6 and Necrosis
 Inflammation Antibodies  >5 publications with MAPK6 and Inflammation
 Lung Diseases Antibodies  >4 publications with MAPK6 and Lung Diseases
 Cardiovascular Diseases Antibodies  >2 publications with MAPK6 and Cardiovascular Diseases
 Fibrosis Antibodies  >2 publications with MAPK6 and Fibrosis
 Cell Transformation, Neoplastic Antibodies  >2 publications with MAPK6 and Cell Transformation, Neoplastic
 Heart Diseases Antibodies  >2 publications with MAPK6 and Heart Diseases

Organs/Tissues associated with MAPK6 sirna

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >12 publications with MAPK6 and Muscle
 Brain Antibodies  >12 publications with MAPK6 and Brain
 Eye Antibodies  >7 publications with MAPK6 and Eye
 Lung Antibodies  >6 publications with MAPK6 and Lung
 Embryonic Tissue Antibodies  >5 publications with MAPK6 and Embryonic Tissue
 Dorsal Root Ganglion Antibodies  >4 publications with MAPK6 and Dorsal Root Ganglion
 Liver Antibodies  >4 publications with MAPK6 and Liver
 Kidney Antibodies  >4 publications with MAPK6 and Kidney
 Skin Antibodies  >3 publications with MAPK6 and Skin
 Thymus Antibodies  >2 publications with MAPK6 and Thymus
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000