MTHFD1L siRNA – Monofunctional C1-Tetrahydrofolate Synthase, Mitochondrial (Mouse)

Name: MTHFD1L siRNA; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS8206071
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8206071

MBS8206071 is an siRNA targeting MTHFD1L. Also known as MTHFD1L siRNA (Mouse). Reactive in Mouse. Host: Synthetic. siRNA to inhibit MTHFD1L expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

MTHFD1L, siRNA

Full Product Name

MTHFD1L siRNA (Mouse)

Product Gene Name

MTHFD1L sirna

Product Synonym Names

FTHFSDC1; Monofunctional C1-tetrahydrofolate synthase mitochondrial; Formyltetrahydrofolate synthetase

Host

Synthetic

Species Reactivity

Mouse

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

MTHFD1L siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

105,729 Da

3D Structure

ModBase 3D Structure for Q3V3R1

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse MTHFD1L gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for MTHFD1L sirna

RNA Interference (RNAi)

Other Notes

Small volumes of MTHFD1L sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MTHFD1L. It may not necessarily be applicable to this product.

NCBI GI #

283135114

NCBI GeneID

270685

NCBI Accession #

NP_001164256.1

UniProt Primary Accession #

Q3V3R1

UniProt Secondary Accession #

Q3TVQ0; Q3V402; Q80V98; Q8CAL0; Q8K2N5

UniProt Related Accession #

Q3V3R1

GenBank Nucleotide #

NM_001170785.1

NCBI Official Full Name

monofunctional C1-tetrahydrofolate synthase, mitochondrial

NCBI Official Synonym Full Names

methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like

NCBI Official Symbol

Mthfd1l

NCBI Official Synonym Symbols

AI647056; Fthfsdc1; 2410004L15Rik

UniProt Protein Name

Monofunctional C1-tetrahydrofolate synthase, mitochondrial

UniProt Synonym Protein Names

Formyltetrahydrofolate synthetase

Protein Family

Monofunctional C1-tetrahydrofolate synthase

UniProt Gene Name

Mthfd1l

UniProt Synonym Gene Names

Fthfsdc1

UniProt Entry Name

C1TM_MOUSE

NCBI Protein Information

monofunctional C1-tetrahydrofolate synthase, mitochondrial

UniProt Comments for MTHFD1L

MTHFD1L: May provide the missing metabolic reaction required to link the mitochondria and the cytoplasm in the mammalian model of one-carbon folate metabolism in embryonic an transformed cells complementing thus the enzymatic activities of MTHFD2. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.4.3; Ligase; Cofactor and Vitamin Metabolism - one carbon pool by folate; Mitochondrial; Carbohydrate Metabolism - glyoxylate and dicarboxylate

Cellular Component: membrane; mitochondrion

Molecular Function: methylenetetrahydrofolate dehydrogenase (NADP+) activity; protein homodimerization activity; nucleotide binding; formate-tetrahydrofolate ligase activity; catalytic activity; ATP binding; ligase activity

Biological Process: folic acid and derivative metabolic process; tetrahydrofolate metabolic process; folic acid and derivative biosynthetic process; formate metabolic process; one-carbon compound metabolic process; 10-formyltetrahydrofolate biosynthetic process

Research Articles on MTHFD1L

1. Deletion of Mthfd1l causes embryonic lethality and neural tube and craniofacial defects in mice.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with MTHFD1L sirna

Reference Product	PubMed Publications
Mthfd2 sirna	>3 publications with MTHFD1L and Mthfd2
Mthfd1 sirna	>2 publications with MTHFD1L and Mthfd1
Ftcd sirna	>1 publications with MTHFD1L and Ftcd

Pathways associated with MTHFD1L sirna

Products by Pathway	Pathway Diagram
C1-unit Interconversion, Eukaryotes Pathway antibodies	C1-unit Interconversion, Eukaryotes Pathway Diagram
C1-unit Interconversion, Eukaryotes Pathway antibodies	C1-unit Interconversion, Eukaryotes Pathway Diagram
Metabolic Pathways antibodies	Metabolic Pathways Diagram
One Carbon Metabolism Pathway antibodies	One Carbon Metabolism Pathway Diagram
One Carbon Pool By Folate Pathway antibodies	One Carbon Pool By Folate Pathway Diagram
One Carbon Pool By Folate Pathway antibodies	One Carbon Pool By Folate Pathway Diagram
Folate Polyglutamylation Pathway antibodies	Folate Polyglutamylation Pathway Diagram
Folate Transformations I Pathway antibodies	Folate Transformations I Pathway Diagram
Folate Transformations II (plants) Pathway antibodies	Folate Transformations II (plants) Pathway Diagram

Diseases associated with MTHFD1L sirna

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>9 publications with MTHFD1L and Cardiovascular Diseases
Nervous System Diseases Antibodies	>8 publications with MTHFD1L and Nervous System Diseases
Brain Diseases Antibodies	>5 publications with MTHFD1L and Brain Diseases
Neural Tube Defects Antibodies	>4 publications with MTHFD1L and Neural Tube Defects
Congenital Abnormalities Antibodies	>4 publications with MTHFD1L and Congenital Abnormalities
Abnormalities, Multiple Antibodies	>2 publications with MTHFD1L and Abnormalities, Multiple
Breast Neoplasms Antibodies	>1 publications with MTHFD1L and Breast Neoplasms

Organs/Tissues associated with MTHFD1L sirna

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>5 publications with MTHFD1L and Blood
Heart Antibodies	>4 publications with MTHFD1L and Heart
Embryonic Tissue Antibodies	>2 publications with MTHFD1L and Embryonic Tissue
Liver Antibodies	>1 publications with MTHFD1L and Liver
Brain Antibodies	>1 publications with MTHFD1L and Brain