NFYB siRNA – Nuclear Transcription Factor Y Subunit Beta (Mouse)

Name: NFYB siRNA; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS8218258
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8218258

MBS8218258 is an siRNA targeting NFYB. Also known as NFYB siRNA (Mouse). Reactive in Mouse. Host: Synthetic. siRNA to inhibit NFYB expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

NFYB, siRNA

Full Product Name

NFYB siRNA (Mouse)

Product Gene Name

NFYB sirna

Product Synonym Names

Nuclear transcription factor Y subunit beta; CAAT box DNA-binding protein subunit B; Nuclear transcription factor Y subunit B; NF-YB

Host

Synthetic

Species Reactivity

Mouse

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

NFYB siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

22,787 Da

3D Structure

ModBase 3D Structure for P63139

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse NFYB gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for NFYB sirna

RNA Interference (RNAi)

Other Notes

Small volumes of NFYB sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NFYB. It may not necessarily be applicable to this product.

NCBI GI #

6754850

NCBI GeneID

18045

NCBI Accession #

NP_035044.1

UniProt Primary Accession #

P63139

UniProt Secondary Accession #

P22569; Q3UK54

UniProt Related Accession #

P63139

GenBank Nucleotide #

NM_010914.2

NCBI Official Full Name

nuclear transcription factor Y subunit beta

NCBI Official Synonym Full Names

nuclear transcription factor-Y beta

NCBI Official Symbol

Nfyb

NCBI Official Synonym Symbols

Cbf-A; AA985999

UniProt Protein Name

Nuclear transcription factor Y subunit beta

UniProt Synonym Protein Names

CAAT box DNA-binding protein subunit B; Nuclear transcription factor Y subunit B; NF-YB

Protein Family

Nuclear transcription factor

UniProt Gene Name

Nfyb

UniProt Synonym Gene Names

NF-YB

UniProt Entry Name

NFYB_MOUSE

NCBI Protein Information

nuclear transcription factor Y subunit beta

UniProt Comments for NFYB

NFYB: Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes. Belongs to the NFYB/HAP3 subunit family.

Protein type: Transcription factor; DNA-binding

Cellular Component: CCAAT-binding factor complex; transcription factor complex; intracellular; nucleus

Molecular Function: DNA binding; protein heterodimerization activity; sequence-specific DNA binding; protein complex binding; transcription factor activity

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent

Research Articles on NFYB

1. deletion of CBF-B activated expression of specific endoplasmic reticulum stress-regulated genes. Inactivation of CBF-B also inhibited expression of C/EBP alpha, an important transcription factor controlling metabolic processes in adult hepatocytes

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with NFYB sirna

Reference Product	PubMed Publications
Nfya sirna	>18 publications with NFYB and Nfya
Nfyc sirna	>11 publications with NFYB and Nfyc
Sp1 sirna	>9 publications with NFYB and Sp1
Rfx5 sirna	>2 publications with NFYB and Rfx5
Ciita sirna	>1 publications with NFYB and Ciita

Pathways associated with NFYB sirna

Products by Pathway	Pathway Diagram
Activation Of Gene Expression By SREBF (SREBP) Pathway antibodies	Activation Of Gene Expression By SREBF (SREBP) Pathway Diagram
Antigen Processing And Presentation Pathway antibodies	Antigen Processing And Presentation Pathway Diagram
Antigen Processing And Presentation Pathway antibodies	Antigen Processing And Presentation Pathway Diagram
HTLV-I Infection Pathway antibodies	HTLV-I Infection Pathway Diagram
HTLV-I Infection Pathway antibodies	HTLV-I Infection Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Lipids And Lipoproteins Pathway antibodies	Metabolism Of Lipids And Lipoproteins Pathway Diagram
Regulation Of Cholesterol Biosynthesis By SREBP (SREBF) Pathway antibodies	Regulation Of Cholesterol Biosynthesis By SREBP (SREBF) Pathway Diagram
TGF-beta Receptor Signaling Pathway antibodies	TGF-beta Receptor Signaling Pathway Diagram
Tuberculosis Pathway antibodies	Tuberculosis Pathway Diagram

Diseases associated with NFYB sirna

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>1 publications with NFYB and Nervous System Diseases
Brain Diseases Antibodies	>1 publications with NFYB and Brain Diseases
Inflammation Antibodies	>1 publications with NFYB and Inflammation
Necrosis Antibodies	>1 publications with NFYB and Necrosis

Organs/Tissues associated with NFYB sirna

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>4 publications with NFYB and Brain
Embryonic Tissue Antibodies	>3 publications with NFYB and Embryonic Tissue
Tongue Antibodies	>1 publications with NFYB and Tongue
Testis Antibodies	>1 publications with NFYB and Testis
Intestine Antibodies	>1 publications with NFYB and Intestine