PDPR siRNA – Pyruvate Dehydrogenase Phosphatase Regulatory Subunit, Mitochondrial (Mouse)

Name: PDPR siRNA; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS8215012
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8215012

MBS8215012 is an siRNA targeting PDPR. Also known as PDPR siRNA (Mouse). Reactive in Mouse. Host: Synthetic. siRNA to inhibit PDPR expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PDPR, siRNA

Full Product Name

PDPR siRNA (Mouse)

Product Gene Name

PDPR sirna

Product Synonym Names

KIAA1990; Pyruvate dehydrogenase phosphatase regulatory subunit mitochondrial; PDPr

Host

Synthetic

Species Reactivity

Mouse

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

PDPR siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

99,230 Da

3D Structure

ModBase 3D Structure for Q7TSQ8

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PDPR gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for PDPR sirna

RNA Interference (RNAi)

Other Notes

Small volumes of PDPR sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PDPR. It may not necessarily be applicable to this product.

NCBI GI #

38142488

NCBI GeneID

319518

NCBI Accession #

NP_938050.1

UniProt Primary Accession #

Q7TSQ8

UniProt Secondary Accession #

Q6ZPF5

UniProt Related Accession #

Q7TSQ8

GenBank Nucleotide #

NM_198308.1

NCBI Official Full Name

pyruvate dehydrogenase phosphatase regulatory subunit, mitochondrial

NCBI Official Synonym Full Names

pyruvate dehydrogenase phosphatase regulatory subunit

NCBI Official Symbol

Pdpr

NCBI Official Synonym Symbols

AU018056; mKIAA1990; 4930402E16Rik

UniProt Protein Name

Pyruvate dehydrogenase phosphatase regulatory subunit, mitochondrial

Protein Family

Pyruvate dehydrogenase phosphatase regulatory

UniProt Gene Name

Pdpr

UniProt Synonym Gene Names

Kiaa1990; PDPr

UniProt Entry Name

PDPR_MOUSE

NCBI Protein Information

pyruvate dehydrogenase phosphatase regulatory subunit, mitochondrial

UniProt Comments for PDPR

PDPR: the inhibitory regulatory subunit of PDP1, a protein phosphoserine phosphatase associated with the mitochondrial matrix. PDP1 activates phosphorylated pyruvate dehydrogenase complex by dephosphorylation and plays a crucial role in switching metabolic flux from glycolysis towards oxidative phosphorylation. Decreases the sensitivity of PDP1 to magnesium ions, and this inhibition is reversed by spermine.

Protein type: Mitochondrial

Cellular Component: mitochondrion; cytoplasm

Molecular Function: oxidoreductase activity

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with PDPR sirna

Products by Pathway	Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Pyruvate Metabolism Pathway antibodies	Pyruvate Metabolism Pathway Diagram
Pyruvate Metabolism And Citric Acid (TCA) Cycle Pathway antibodies	Pyruvate Metabolism And Citric Acid (TCA) Cycle Pathway Diagram
Regulation Of Pyruvate Dehydrogenase (PDH) Complex Pathway antibodies	Regulation Of Pyruvate Dehydrogenase (PDH) Complex Pathway Diagram
The Citric Acid (TCA) Cycle And Respiratory Electron Transport Pathway antibodies	The Citric Acid (TCA) Cycle And Respiratory Electron Transport Pathway Diagram

Diseases associated with PDPR sirna

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>1 publications with PDPR and Nervous System Diseases
Neoplasms, Experimental Antibodies	>1 publications with PDPR and Neoplasms, Experimental

Organs/Tissues associated with PDPR sirna

Organ/Tissue Name	Pubmed Publications
Intestine Antibodies	>2 publications with PDPR and Intestine
Skin Antibodies	>2 publications with PDPR and Skin
Kidney Antibodies	>2 publications with PDPR and Kidney
Brain Antibodies	>2 publications with PDPR and Brain
Blood Antibodies	>1 publications with PDPR and Blood