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PSMC1 siRNA – 26s Protease Regulatory Subunit 4 (Mouse)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8201605

MBS8201605 is an siRNA targeting PSMC1. Also known as PSMC1 siRNA (Mouse). Reactive in Mouse. Host: Synthetic. siRNA to inhibit PSMC1 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PSMC1, siRNA
Full Product Name
PSMC1 siRNA (Mouse)
Product Gene Name
PSMC1 sirna
Product Synonym Names
26S protease regulatory subunit 4; P26s4; 26S proteasome AAA-ATPase subunit RPT2; Proteasome 26S subunit ATPase 1
Host
Synthetic
Species Reactivity
Mouse
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
PSMC1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
49,185 Da
3D Structure
ModBase 3D Structure for P62192
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PSMC1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for PSMC1 sirna
RNA Interference (RNAi)

Other Notes

Small volumes of PSMC1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PSMC1. It may not necessarily be applicable to this product.

NCBI GI #
6679501
NCBI GeneID
19179
NCBI Accession #
NP_032973.1
UniProt Primary Accession #
P62192
UniProt Secondary Accession #
P49014; Q03527; Q96AZ3
UniProt Related Accession #
P62192
GenBank Nucleotide #
NM_008947.3
NCBI Official Full Name
26S protease regulatory subunit 4
NCBI Official Synonym Full Names
protease (prosome, macropain) 26S subunit, ATPase 1
NCBI Official Symbol
Psmc1
NCBI Official Synonym Symbols
S4; rpt2; P26s4; Rpt2/S4; AI325227
UniProt Protein Name
26S protease regulatory subunit 4
UniProt Synonym Protein Names
26S proteasome AAA-ATPase subunit RPT2; Proteasome 26S subunit ATPase 1
UniProt Gene Name
Psmc1
UniProt Synonym Gene Names
P26s4
UniProt Entry Name
PRS4_MOUSE
NCBI Protein Information
26S protease regulatory subunit 4

UniProt Comments for PSMC1

RPT2: The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex. Belongs to the AAA ATPase family.

Protein type: Protease; Proteasome complex

Cellular Component: nucleoplasm; proteasome complex; membrane; proteasome regulatory particle; cytoplasm; cell junction; nucleus

Molecular Function: hydrolase activity; TATA-binding protein binding; nucleotide binding; ATP binding

Biological Process: protein catabolic process
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Research Articles on PSMC1

1. In Psmc1(+/-)primary mouse embryonic fibroblasts we found decreased PSMC1 protein expression, altered assembly of the 26S proteasome and G2/M cell cycle arrest.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PSMC1 sirna

 Reference Product  PubMed Publications
 Psmc4 sirna  >3 publications with PSMC1 and Psmc4
 Psmc2 sirna  >2 publications with PSMC1 and Psmc2
 Psmc3 sirna  >1 publications with PSMC1 and Psmc3
 Psmd2 sirna  >1 publications with PSMC1 and Psmd2
 Psma7 sirna  >1 publications with PSMC1 and Psma7
 Psmd4 sirna  >1 publications with PSMC1 and Psmd4

Pathways associated with PSMC1 sirna

 Products by Pathway  Pathway Diagram
 APC/C-mediated Degradation Of Cell Cycle Proteins Pathway antibodies  APC/C-mediated Degradation Of Cell Cycle Proteins Pathway Diagram
 APC/C:Cdc20 Mediated Degradation Of Securin Pathway antibodies  APC/C:Cdc20 Mediated Degradation Of Securin Pathway Diagram
 APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies  APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
 APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway antibodies  APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway Diagram
 APC:Cdc20 Mediated Degradation Of Cell Cycle Proteins Prior To Satisfation Of The Cell Cycle Checkpoint Pathway antibodies  APC:Cdc20 Mediated Degradation Of Cell Cycle Proteins Prior To Satisfation Of The Cell Cycle Checkpoint Pathway Diagram
 ARMS-mediated Activation Pathway antibodies  ARMS-mediated Activation Pathway Diagram
 AUF1 (hnRNP D0) Destabilizes MRNA Pathway antibodies  AUF1 (hnRNP D0) Destabilizes MRNA Pathway Diagram
 Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies  Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
 Activation Of NF-kappaB In B Cells Pathway antibodies  Activation Of NF-kappaB In B Cells Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram

Diseases associated with PSMC1 sirna

 Disease Name  Pubmed Publications
 Prostatic Diseases Antibodies  >2 publications with PSMC1 and Prostatic Diseases
 Hepatitis Antibodies  >1 publications with PSMC1 and Hepatitis
 Liver Diseases Antibodies  >1 publications with PSMC1 and Liver Diseases
 Hyperplasia Antibodies  >1 publications with PSMC1 and Hyperplasia

Organs/Tissues associated with PSMC1 sirna

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >4 publications with PSMC1 and Brain
 Blood Antibodies  >3 publications with PSMC1 and Blood
 Muscle Antibodies  >2 publications with PSMC1 and Muscle
 Prostate Antibodies  >2 publications with PSMC1 and Prostate
 Spleen Antibodies  >1 publications with PSMC1 and Spleen
 Lung Antibodies  >1 publications with PSMC1 and Lung
 Skin Antibodies  >1 publications with PSMC1 and Skin
 Liver Antibodies  >1 publications with PSMC1 and Liver
 Heart Antibodies  >1 publications with PSMC1 and Heart
 Testis Antibodies  >1 publications with PSMC1 and Testis
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