PTH2 siRNA – Tuberoinfundibular Peptide of 39 Residues Preproprotein (Mouse)

Name: PTH2 siRNA; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS8231043
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8231043

MBS8231043 is an siRNA targeting PTH2. Also known as PTH2 siRNA (Mouse). Reactive in Mouse. Host: Synthetic. siRNA to inhibit PTH2 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PTH2, siRNA

Full Product Name

PTH2 siRNA (Mouse)

Product Gene Name

PTH2 sirna

Product Synonym Names

TIP39; TIPF39; Tuberoinfundibular peptide of 39 residues; TIP39; Parathyroid hormone 2

Host

Synthetic

Species Reactivity

Mouse

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

PTH2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

11,022 Da

3D Structure

ModBase 3D Structure for Q91W27

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PTH2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for PTH2 sirna

RNA Interference (RNAi)

Other Notes

Small volumes of PTH2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PTH2. It may not necessarily be applicable to this product.

NCBI GI #

16716587

NCBI GeneID

114640

NCBI Accession #

NP_444486.1

UniProt Primary Accession #

Q91W27

UniProt Secondary Accession #

Q91ZA0

UniProt Related Accession #

Q91W27

GenBank Nucleotide #

NM_053256.2

NCBI Official Full Name

tuberoinfundibular peptide of 39 residues preproprotein

NCBI Official Synonym Full Names

parathyroid hormone 2

NCBI Official Symbol

Pth2

NCBI Official Synonym Symbols

Tip39; Tifp39

UniProt Protein Name

Tuberoinfundibular peptide of 39 residues

UniProt Synonym Protein Names

Parathyroid hormone 2

Protein Family

Peptidyl-tRNA hydrolase

UniProt Gene Name

Pth2

UniProt Synonym Gene Names

Tip39; Tipf39; TIP39

UniProt Entry Name

TIP39_MOUSE

NCBI Protein Information

tuberoinfundibular peptide of 39 residues

NCBI Summary for PTH2

This gene encodes the precursor of a peptide hormone that shares sequence similarity with the parathyroid hormone. This gene is expressed in various regions of the brain where it plays a role in the release of pituitary hormones, anxiety and nociception. The encoded precursor protein is proteolytically processed to generate the biologically active neuropeptide. Mice lacking the encoded protein display increased fear and anxiety after exposure to stressful events, and decreased sensitivity to pain. [provided by RefSeq, Aug 2015]

UniProt Comments for PTH2

PTH2: Plays a role as a potent and selective agonist of PTH2R resulting in adenyl cyclase activation and intracellular calcium levels elevation. Induces protein kinase C beta activation, recruitment of beta-arrestin and PTH2R internalization. May inhibit cell proliferation via its action on PTH2R activation. Neuropeptide which may also have a role in spermatogenesis. May activate nociceptors and nociceptive circuits. Belongs to the parathyroid hormone family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular region

Molecular Function: receptor binding

Biological Process: neuropeptide signaling pathway; cAMP biosynthetic process

Research Articles on PTH2

1. Data indicate that the nociceptive phenotype of TIP39-knockout {KO) mice was similar to the PTH2R-KO.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PTH2 sirna

Reference Product	PubMed Publications
Pth sirna	>51 publications with PTH2 and Pth
Pthlh sirna	>10 publications with PTH2 and Pthlh
Pth1r sirna	>8 publications with PTH2 and Pth1r
Pth2r sirna	>7 publications with PTH2 and Pth2r

Pathways associated with PTH2 sirna

Products by Pathway	Pathway Diagram
Class B/2 (Secretin Family Receptors) Pathway antibodies	Class B/2 (Secretin Family Receptors) Pathway Diagram
G Alpha (s) Signalling Events Pathway antibodies	G Alpha (s) Signalling Events Pathway Diagram
GPCR Downstream Signaling Pathway antibodies	GPCR Downstream Signaling Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram
Signal Transduction Pathway antibodies	Signal Transduction Pathway Diagram
Signaling By GPCR Pathway antibodies	Signaling By GPCR Pathway Diagram

Diseases associated with PTH2 sirna

Disease Name	Pubmed Publications
Disease Models, Animal Antibodies	>7 publications with PTH2 and Disease Models, Animal
Necrosis Antibodies	>5 publications with PTH2 and Necrosis
Kidney Diseases Antibodies	>3 publications with PTH2 and Kidney Diseases
Cardiovascular Diseases Antibodies	>2 publications with PTH2 and Cardiovascular Diseases
Hyperplasia Antibodies	>2 publications with PTH2 and Hyperplasia
Anemia Antibodies	>2 publications with PTH2 and Anemia
Breast Neoplasms Antibodies	>2 publications with PTH2 and Breast Neoplasms
Neoplasms, Experimental Antibodies	>1 publications with PTH2 and Neoplasms, Experimental
Carcinoma, Hepatocellular Antibodies	>1 publications with PTH2 and Carcinoma, Hepatocellular
Liver Neoplasms Antibodies	>1 publications with PTH2 and Liver Neoplasms